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Functional human neurons, derived from induced pluripotent stem cells, reprogrammed from patients with MECP2 duplication syndrome showed remarkably differences in certain cortical layer markers compared to controls. Cells were immunostained for Tuj1 (green), CTIP2 (red) and nuclei was stained with fluorescent dye DAPI (blue). Original magnification is ×20. Image courtesy from the Muotri & Van Esch laboratories. For more info on this topic, please refer to the article by Muotri et al on pages 178–188.