Volume 19 Issue 5, May 2014

Cover Legend: Localization of interleukin-1β in peripheral and brain-transmigrating neutrophils. Interleukin (IL)-1 β expressing neutrophils transmigrate into the brain after peripheral inflammation by LPS, contributing to the levels of this cytokine in the central nervous system. Confocal fluorescence microscopy images of naïve, bone-marrow isolated (top panels) and brain-transmigrated (lower panels) neutrophils. In the braininvading neutrophils (Ly6B positive cells, green signal) the localization of pro-IL-1β immunoreactivity (red signal) is localized mostly in the cell nucleus (DAPI stained, blue signal), as can be observed in the merged (rightmost panels); whereas in naïve neutrophils, pro-IL-1 b staining was primarily localized in the cytoplasm. For more info on this topic, please refer to the article by Aguilar-Valles et al. on pages 599–606.