Volume 17 Issue 12, December 2012

Two-week neuronal cultures of in vitro differentiated mouse induced pluripotent stem cells are electrophysiologically active. In this figure, stem cell-derived neurons heterozygous for the Mecp2308 mutation express the dendritic marker MAP2 (in green) with DAPI nuclear counterstain in blue. A representative trace of a train of action potentials (top left in red) can be detected at this time using whole-cell patch-clamp recording techniques. For more info on this topic, please refer to the article by Farra et al. on pages 1261–1271.