Volume 99 Issue 11, November 2019

Volume 99 Issue 11

The cover shows cardiac sections from a 4C30 mouse, which harbors sialyltransferase transgenes, and from a wild-type mouse. Stain: Masson’s trichrome. For more information, see the paper by Nagai-Okatani et al, p. 1749, this issue.

Inside the USCAP Journals

Article

Technical Report

  • Technical Report |

    Protein misfolding cyclic amplification (PMCA) is a technique able to detect minute amount of disease-related prion protein (PrPD), but limited results have been obtained with human prions with the exception of variant Creutzfeldt-Jakob disease (variant CJD). The study demonstrates that the use of substrates with deglycosylated PrP strongly increases PrPD amplification efficiency by PMCA for all tested CJD subtypes. The enhanced PMCA efficiency may allow for the developing of a sensitive, non-invasive, diagnostic tests for the different CJD subtypes based on body fluids or easily accessible peripheral tissues.

    • Manuel V. Camacho
    • , Glenn Telling
    • , Qingzhong Kong
    • , Pierluigi Gambetti
    •  & Silvio Notari
  • Technical Report |

    The authors propose that Wisteria floribunda agglutinin (WFA) staining on failing heart sections may be useful for quantitative assessment of cardiac fibrogenic activity. The fibrosis-specific WFA staining is mainly due to the WFA binding to fibrogenesis-related extracellular matrix N-glycoproteins. It is expected that cardiac WFA-binding glycoproteins may be circulating glyco-biomarkers for cardiac fibrogenesis.

    • Chiaki Nagai-Okatani
    • , Mitsuhiro Nishigori
    • , Takashi Sato
    • , Naoto Minamino
    • , Hiroyuki Kaji
    •  & Atsushi Kuno