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Volume 102 Issue 7, July 2022

The cover shows MIBI-TOF imaging data from an archival human tissue microarray of squamous cell carcinoma. For more information, see the paper by Liu et al, this issue (p 762).

Inside the USCAP Journals

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Technical Report

  • Multiplexed ion beam imaging by time-of-flight (MIBI-TOF) uses secondary ion mass spectrometry to image dozens of proteins simultaneously in the same tissue section. The authors undertook a validation study, assessing concordance across serial sections of a tissue microarray that were independently imaged by MIBI-TOF or single-plex immunohistochemistry. They demonstrate that MIBI-TOF can generate consistent and quantitative annotations of clinically relevant cell states in archival human tissue and present a scalable framework for benchmarking multiplexed immunohistochemical approaches.

    • Candace C. Liu
    • Marc Bosse
    • Michael Angelo
    Technical Report
  • Siglec-15 is normally expressed by myeloid cells and upregulated in some human cancers and represents a promising new target for immunotherapy. The aim of this study was to develop an immunohistochemical assay for Siglec-15 to be used as a companion diagnostic for future clinical trials. Here, the authors created and validated an assay with a novel recombinant antibody to the cytoplasmic domain of Siglec-15. This study may support development of a companion diagnostic assay to enrich for patients expressing the Siglec-15 target for therapy.

    • Saba Shafi
    • Thazin Nwe Aung
    • David L. Rimm
    Technical Report
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