The cover shows immunofluorescent double staining of ZO-1 and RPE65 in retinal pigment epithelial cells. For more information, see the paper by Lennikov et al, p 232, this issue.

In this issue, the paper by Yamazaki et al describes how continuous formation of small clusters of LGR5-positive cells contribute to tumor growth in colorectal cancer. The cover shows distribution of LGR5-positive cells (green) and HLA-DMA (red) in 3D structures.