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Sickle cell disease (SCD) is associated with deficits in revascularization following vascular injury and the role of nitric oxide (NO) bioavailability in SCD vasculopathies is controversial. This study tests L-arginine and NO-hydrogel treatments in a model of vascular insufficiency and finds that neither therapy improves recovery in SCD mice. In fact, delivery of NO at the site of ischemia increases oxidative stress and worsens outcomes, highlighting potential limitations of NO-targeted therapeutics in SCD.
Multiplexed ion beam imaging by time-of-flight (MIBI-TOF) uses secondary ion mass spectrometry to image dozens of proteins simultaneously in the same tissue section. The authors undertook a validation study, assessing concordance across serial sections of a tissue microarray that were independently imaged by MIBI-TOF or single-plex immunohistochemistry. They demonstrate that MIBI-TOF can generate consistent and quantitative annotations of clinically relevant cell states in archival human tissue and present a scalable framework for benchmarking multiplexed immunohistochemical approaches.
NORAD, a non-coding RNA activated by DNA damage, is highly expressed in osteosarcoma cells and tissue. The authors show that extracellular vesicles (EVs) derived from bone mesenchymal stem cells (BMSCs) deliver NORAD to osteosarcoma cells to regulate the miR-30c-5p/ Krueppel-like factor 10 axis, thereby accelerating the progression and metastasis of osteosarcoma.
In this study, the technical feasibility and the diagnostic value of SNP array analysis on 171 core needle biopsies (CNB) from soft tissue and bone tumors was evaluated. The analysis succeeded technically in 98% of the cases. The copy number profiles were compatible with the CNB diagnoses in 87% of the cases and was in 77% of the cases representative for the whole lesion.
Bronchopulmonary dysplasia (BPD) is still the most common challenge in preterm neonates. The authors found that reactive oxygen species and sCD146 are increased in preterm peripheral blood samples. They then show that the CD146-HIF-1α axis contributes to alveolarization and propose that CD146 may be a novel candidate in BPD therapy.