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Welcome to Laboratory Investigation

Advancing the understanding of human disease

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  • Stained cells

    This collection, curated by Ho-Keung Ng of the Chinese University of Hong Kong, focuses on the underlying pathogenetic and molecular processes that contribute to brain tumors.

  • Immunofluorescent staining of human melanocytic specimens

    This Collection highlights the newest top-viewed content from Laboratory Investigation. Updated each month, we hope you enjoy reading this selection of articles. Laboratory Investigation aims to publish high-quality original research in all biomedical disciplines relating to the understanding of human disease and the application of new methods to the diagnosis of disease.

  • Focus on China Collection Cover

    The editors of Laboratory Investigation are proud to present a Web Focus that showcases research by authors from institutions across China. We selected recent papers to highlight the significant contributions of Chinese scientists and to introduce the journal to an even wider audience.

  • Pseudo-color rendering of fluorescence-like representations of non-small cell lung cancer

    Brightfield microscopy is the preferred method of pathologists for diagnosing solid tumors, utilizing common staining techniques such as hematoxylin and eosin staining and immunohistochemistry. This collection of articles describe new ways to process samples and interpret data generated by histopathology and immunohistochemistry, as well as new technologies that complement these methods.

Laboratory Investigation is a Transformative Journal; authors can publish using the traditional publishing route OR via immediate gold Open Access.

Our Open Access option complies with funder and institutional requirements.

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  • The authors present a workflow that allows the simultaneous measurement of the whole exome and the transcriptome by next-generation sequencing from formalin-fixed paraffin-embedded tissue sections that were analyzed by matrix-assisted laser desorption ionization mass spectrometry imaging. The data and analyses demonstrate the feasibility and reproducibility of this approach, which expands the possibilities of multi-omics integration in cancer research.

    • Lisa Kreutzer
    • Peter Weber
    • Kristian Unger
    Technical Report Open Access
  • For precancerous/endometrial cancer patients with fertility maintain desire, progestin resistance is the main obstacle of conservative therapy. The authors found that brusatol, as a natural compound, suppresses progestin metabolism through regulating the NRF2-TET1-AKR1C1 pathway to sensitize precancerous/endometrial cancers to progestin and relieve progestin resistance. This study indicates that progestin combined with brusatol may enhance the treatment effects and that AKR1C1 expression patterns may serve as an important biomarker of progestin resistance in endometrial cancer.

    • Meiyan Hu
    • Di Sun
    • Zhenbo Zhang
    Article
  • Non-small cell lung cancer (NSCLC) is the most common type of lung cancer and is associated with limited treatment options. This study elucidates the role of KLHL17 in the development and progression of NSCLC using clinical samples and NSCLC cell lines. The results show that upregulated KLHL17 in NSCLC promotes the proliferation and migration of tumor by activating Ras/MAPK signaling pathway, and suggest that KLHL17 may be a novel therapeutic target for the treatment of NSCLC.

    • Zongang Liu
    • Mengnan Zhao
    • Xueshan Qiu
    Article
  • The authors describe a regulatory mechanism for ferroptosis sensitivity that is dependent on circST6GALNAC6 expression levels in bladder cancer. CircST6GALNAC6 inhibits HSPB1 and promotes cell ferroptosis by blocking the phosphorylation site (Ser-15) of HSBP1 and activating the P38 MAPK signaling pathway. Enhancing the expression of circST6GALNAC6 to promote ferroptosis, or using circST6GALNAC6 as a biomarker of ferroptosis sensitivity, is significant for the development and application of ferroptosis intervention methods.

    • Li Wang
    • Shuiqing Wu
    • Xuan Zhu
    Article
  • Hyperglycemia induces endoplasmic reticulum stress and increases expression of MAPK10, and 4-phenylbutyric treatment and the knockdown of MAPK10 both alleviate hyperglycaemia-induced myocardial dysfunction, respectively. Therefore, the present study sheds new light on the novel role of MAPK10 in hyperglycemia-induced myocardial dysfunction in db/db mice.

    • Ya-Wen Deng
    • Fei Liu
    • Yun-Long Xia
    Article Open Access
  • Proteomics plays a vital role in biomedical research in the post-genomic era. With the technological revolution and emerging computational tools, proteomic methodology has evolved rapidly in the past decade and shed light on solving complicated biomedical problems. Thus, this mini-review summarizes existing and emerging high-throughput proteomics methodologies, including mass spectrometry, protein pathway array, next-generation tissue microarrays, single-cell proteomics, single-molecule proteomics, Luminex, Simoa and OLINK Proteomics.

    • Miao Cui
    • Chao Cheng
    • Lanjing Zhang
    Review Article
  • This study shows the potential application of c-Jun phosphorylation upon cisplatin treatment as a biomarker for cisplatin sensitivity in combination with patient-derived tumor organoids (PDOs). We also compared the responses of 6 PDOs to cisplatin with the therapeutic effect of neoadjuvant chemotherapy in 6 matched patients with esophageal squamous cell carcinoma.

    • Yoshiyuki Tsukamoto
    • Shusaku Kurogi
    • Masatsugu Moriyama
    Article
  • GPM6A expression is markedly suppressed in hepatocellular carcinoma and suppresses proliferation and migration of hepatoma cells. Moreover, its low expression is most likely attributed to upregulated miRNA-96-5p but not gene mutation or changes in methylation. These findings indicate that GPM6A may function as an inhibitory gene in chronic information, fibrosis and oncogenic transformation.

    • Zong-Rui Li
    • Gang Xu
    • Jian Wu
    Article