Welcome to Laboratory Investigation

Advancing the understanding of human and experimental disease

Laboratory Investigation is a Transformative Journal; authors can publish using the traditional publishing route OR Open Access.

Our Open Access option complies with funder and institutional requirements.


  • Human corneal keratocytes (HCK) are essential for maintaining corneal structure and transparency. This study shows that the natural antioxidant L-carnitine inhibits stromal scarring by suppressing injury-induced intrinsic transient receptor potential vanilloid type 1 (TRPV1) activity that is linked to induction of myofibroblast transdifferentiation in HCK cells. Blocking TRPV1 activation on keratocytes may therefore be a viable approach to suppress corneal opacification in a clinical setting.

    • Elizabeth Turan
    • Monika Valtink
    • Stefan Mergler
    Article Open Access
  • The breast cancer immune microenvironment was analyzed with the nanoString GeoMx® Digital Spatial Profiler (DSP) in cases from the Carolina Breast Cancer Study. Basal-like breast cancers showed increased expression of markers for regulatory T cells. The results were highly reproducible between whole sections and tissue microarrays.

    • Andrea Walens
    • Linnea T. Olsson
    • Benjamin C. Calhoun
    Technical Report
  • The authors developed a deep-learning-based ductal carcinoma in situ (DCIS) grading system that achieved performance similar to expert observers. To the best of our knowledge, this is the first automated system for the grading of DCIS that could assist pathologists by providing robust and reproducible second opinions on DCIS grade.

    • Suzanne C. Wetstein
    • Nikolas Stathonikos
    • Mitko Veta
    Technical Report Open Access
  • Dimethylarginine dimethylamino hydrolase-1 (DDAH-1), as the critical enzyme responsible for asymmetric dimethylarginine (ADMA) degradation, serves as a protective factor for ischemic stroke. DDAH-1 protects ischemia-induced disruption of blood-brain-barrier via regulating ADMA level and preventing tight junction proteins degradation. The supplementation of L-arginine helps restore the function of DDAH-1.

    • Yichen Zhao
    • Xiaoye Ma
    • Yanxin Zhao
  • Most current biomedical datasets are rectangular in shape and have few missing data, but the sample sizes are very large. Rigorous analyses of these huge datasets demand considerably more efficient and more accurate machine-learning algorithms to classify outcomes. This paper aims to determine the performance and efficiency of classifying multi-category outcomes of such rectangular data.

    • Fei Deng
    • Jibing Huang
    • Lanjing Zhang
  • This manuscript describes a methodology to quantify the abnormalities in digital cytology images. This automatic AI-system incorporates deep learning structures, mathematical algorithms, and image processing methods to locate and segment abnormal and suspicious cells. Characterized as more informative, objective, and reproducible, it has the potential to assist clinical practice.

    • Jing Ke
    • Yiqing Shen
    • Fusong Jiang