George F Murphy

Welcome to Laboratory Investigation

Advancing the understanding of human and experimental disease

Latest Research

  • Review Article |

    The increasing interest of miRNAs in clinical practice has led to an unmet need for assays that can rapidly and accurately measure miRNAs at the point of care. This review discusses the clinical feasibility of different miRNA amplification-based assays, from conventional methods to alternative technologies such as isothermal amplification, paper-based, oligonucleotide-templated reaction, nanobead-based, electrochemical signaling-based, and microfluidic chip-based strategies.

    • Vivek Priy Dave
    • , Tien Anh Ngo
    • , Anna-Karin Pernestig
    • , Diana Tilevik
    • , Krishna Kant
    • , Trieu Nguyen
    • , Anders Wolff
    •  & Dang Duong Bang
  • Article |

    Epithelial cell transforming sequence 2 (ECT2), a guanine nucleotide exchange factor, is localized in both the nucleus and cytoplasm of cell lines and resected lung adenocarcinomas. In this article, the authors show that cytoplasmic expression of ECT2 is associated with poor outcomes and that positive P-ECT2(T790) staining correlates with cytoplasmic ECT2 expression. Furthermore, cytoplasmic ECT2 expression promotes the malignant progression of lung adenocarcinoma.

    • Zeinab Kosibaty
    • , Yoshihiko Murata
    • , Yuko Minami
    • , Tomoko Dai
    • , Junko Kano
    • , Ryota Matsuoka
    • , Noriyuki Nakano
    •  & Masayuki Noguchi
  • Article |

    Polyploid giant cancer cells (PGCCs) have the properties of cancer stem cells, and cell-cycle-related proteins, including cyclin B1, CDC25B, and CDC25C play an important role in regulating the formation of PGCCs. This study shows that the phosphorylated proteins Chk2, and Aurora-A kinase regulate the expression and subcellular localization of cyclin B1, CDC25B, and CDC25C. The subcellular localization of these cell-cycle-related proteins is associated with pathologic grade and metastasis of tumors in cases of human breast cancer and ovarian cancer.

    • Fei Fei
    • , Jie Qu
    • , Kai Liu
    • , Chunyuan Li
    • , Xinlu Wang
    • , Yuwei Li
    •  & Shiwu Zhang
  • Article |

    The authors evaluated the effect of global deletion of NADPH oxidase 4 (NOX4) on TGF-β-induced fibrosis in mouse skin and lungs. Analysis of C57BL6/J and Nox4 knockout mice skin and lung sections by histopathology, measurement of hydroxyproline content and profibrotic gene expression levels showed that Nox4 deletion abrogated TGF-β1-induced tissue fibrosis.

    • Peter J. Wermuth
    • , Fabian A. Mendoza
    •  & Sergio A. Jimenez
  • Review Article |

    Chronic obstructive pulmonary disease (COPD) is a common, destructive and pre-malignant airway disease often seen in smokers. We know remarkably little about the underlying driving mechanisms. Scientists have recently made important observations indicating that epithelial to mesenchymal transition (EMT) is central to these pathologies, with heparin-binding EGF-like growth factor as an emerging novel therapeutic target to ameliorate EMT in smokers and patients with COPD.

    • Mathew Suji Eapen
    • , Pawan Sharma
    • , Isobel E. Thompson
    • , Wenying Lu
    • , Stephen Myers
    • , Philip M. Hansbro
    •  & Sukhwinder Singh Sohal

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