Welcome to Laboratory Investigation

Advancing the understanding of human and experimental disease


  • Visualization of segmented steatosis droplets

    Biology has evolved greatly in the past decade as high-throughput technologies were developed and applied to various biological disciplines. These technologies have generated an unprecedented amount and new types of biological data and how to make sense of “big data” is an emerging technological and conceptual challenge.

  • Immunofluorescent staining of human melanocytic specimens

    This Collection highlights the newest top-viewed content from Laboratory Investigation. Updated each month, we hope you enjoy reading this selection of articles. Laboratory Investigation aims to publish high-quality original research in all biomedical disciplines relating to the understanding of human disease and the application of new methods to the diagnosis of disease.

  • Pseudo-color rendering of fluorescence-like representations of non-small cell lung cancer

    Brightfield microscopy is the preferred method of pathologists for diagnosing solid tumors, utilizing common staining techniques such as hematoxylin and eosin staining and immunohistochemistry. This collection of articles describe new ways to process samples and interpret data generated by histopathology and immunohistochemistry, as well as new technologies that complement these methods.

  • Immunofluorescent staining of a mouse cardiac section with lectins

    The editors of Laboratory Investigation present a collection of recent papers that explore the relationships between changes in the mammalian glycome and pathobiology. These studies describe new roles for glycans in heart disease, neurodegenerative diseases, genetic disorders, and several types of cancer.

Laboratory Investigation is a Transformative Journal; authors can publish using the traditional publishing route OR via immediate gold Open Access.

Our Open Access option complies with funder and institutional requirements.


  • It is unclear how to best classify cancer outcomes using ‘omic data. We developed a multimetric feature-selection based multinomial logistic regression model that outperformed random forest models in classifying 4-category outcome of colorectal cancer. Adding microsatellite instability and oncogenic-driver data to clinical and transcriptomic data improves models’ performances, with pathologic staging, HBS1L, TSPYL4, and TP53TG3B as important features. Interestingly, precision and recall of tuned algorithms change as the feature number changes, but accuracy does not.

    • Catherine H. Feng
    • Mary L. Disis
    • Lanjing Zhang
  • Extensive morphological analysis demonstrates the importance of tunneling nanotubes for intercellular communication in osteoclast differentiation, especially in the fusion process of osteoclast precursors. Successful detection of nanotubes was also demonstrated in cultured primary osteoclasts resorbing dentin slices, and in osteoclasts in bone destruction sites of arthritic rats. Tunneling nanotubes are important for the differentiation of osteoclasts, both in vitro and in vivo.

    • Jing-Qi Zhang
    • Akira Takahashi
    • Toshio Kukita
  • The authors investigated the contribution of yes associated protein (YAP) and transcriptional coactivator with PDZ-binding motif (TAZ) in osteocyte mechanotransduction. In a 3-D osteocyte compression model, they show that YAP/TAZ signaling is activated, is required for osteocyte mechano-responsiveness, and regulates the expression profile of target genes as shown by RNAseq analysis and the control of chemokine expression.

    • Mylene Zarka
    • Francois Etienne
    • Martine Cohen-Solal
  • In this study, the authors demonstrated that bioactive glass promotes granulation formation, collagen deposition and angiogenesis to accelerate wound healing. Bioactive glass down-regulaties the connexin 43/reactive oxygen species signaling pathway to inhibit endothelial cell pyroptosis in vitro and in vivo.

    • Kailun Zhang
    • Bo Chai
    • Ke Xu
  • Chronic mineral-oil intraperitoneal administration induces hepatic inflammation through innate immune responses via myeloid cell activation. Lcn2 null mice develop less inflammation due to defective genes involved in myeloid cell activation, and downregulated gene sets for collagen-containing extracellular matrix, leading to mitigation of the liver fibrosis. Lcn2 null mice show enriched expression of genes responsible for DNA damage and cell cycle DNA replication compared to wild-type upon chronic CCl4 liver injury.

    • Erawan Borkham-Kamphorst
    • Ute Haas
    • Ralf Weiskirchen
    Article Open Access
  • Ameloblastoma (AB) is the most common benign odontogenic tumor. In this study, we investigated the expression and distribution of acetylated α-tubulin and αTAT1 in AB specimens. We analyzed tubulin acetylation caused by αTAT1 activation in a human AB cell line, AM-1. Results of the histopathological and in vitro studies clarified that TAK1 was phosphorylated by TGF-β stimulation, then, induced tubulin acetylation via αTAT1 activation, which subsequently activated the migration and invasion of AB cells.

    • Shohei Yoshimoto
    • Hiromitsu Morita
    • Shuichi Hashimoto
    Article Open Access
  • Vitamin D has recently emerged as a neurosteroid and a regulator of various physiological functions. It has been widely reported to promote tumor-suppressive effects, however this proposal remains controversial. This article reviews the anti-tumoral mechanisms of vitamin D in glioblastoma, current evidence of its therapeutic application as a supplement to standard chemotherapy, and its potential applications for cancer prevention; it endeavors to offer insight into new means of overcoming chemoresistance and improving glioma patient survival.

    • Carmen Sze-Ching Lo
    • Karrie Mei-Yee Kiang
    • Gilberto Ka-Kit Leung
    Review Article
  • The 5th edition of WHO Classification of Tumors of the Central Nervous System adopted new molecular markers into the revised grading criteria of IDH-mutant and -wild-type astrocytoma, i.e., the CDKN2A/B homozygous deletion for IDH-mutant astrocytoma and the following three criteria for IDH-wild-type astrocytoma: the concurrent gain of whole chromosome 7 and loss of whole chromosome 10, TERT promoter mutations, and EGFR amplification, as independent molecular markers of the highest grade.

    • Takashi Komori
    Review Article