In this Perspective, the authors summarize the current knowledge on the post-COVID-19 condition, focusing on the neurological manifestations, and discuss the applicability of existing animal models to recapitulate human condition.

Poor replicability in animal research can be a result of low external validity driven by rigorous standardization of study populations. This study investigates how heterogenization of study populations by using mice from different breeding sites might affect the replicability of animal studies conducted in a single facility. The findings suggest that heterogenization by breeding site has limited capacity to improve replicability in animal research.

The 3R principles provide an ethical framework for animal research throughout the world. However, despite the increasing awareness of these principles, there is still a lot of room for improving their implementation, especially when it comes to reduction. By combining Bayesian statistics with a shift in experimental design, here we present an entirely new idea to reduce animal numbers within experiments.

Meijs et al. perform an electrophysiological investigation of cortical responses in a pig nerve injury model, showing the role of layer III–VI neurons in altered primary somatosensory cortex excitability after nerve injury.

Severe combined immunodeficiency (SCID) pigs do not live long enough to reproduce naturally. Monarch et al. show that reconstituting the immune system of SCID piglets with fetal bone allografts extends their lifespan, allowing them to reach puberty.

This study shows that using gene-editing technology in oocytes using Platinum TALEN is an effective method for producing genetically modified marmosets by exon skipping.

Free flap is widely used in head and neck reconstructive surgery, but fluid management of surgical patients remains controversial. Here, Thiem et al. used a pig model to monitor the effects of volume management on free flap perfusion and metabolism.

Pairing palatable formulations with micropipette dosing for administration of water-insoluble drugs such as tamoxifen in mice promotes voluntary consumption without loss of efficacy compared to more invasive administration methods.

This study reports the use of fluorescently labeled collagen hybridizing peptides to directly image collagen remodeling and monitor fibrosis in two mouse models of ocular neovascularization.