Goblet cell–associated antigen passages can deliver luminal substances to antigen-presenting cells to induce antigen-specific T cell responses. This protocol describes how to identify and quantify intestinal epithelial cells that have the capacity to take up luminal substances, by intraluminal injection of fluorescent dextran, tissue sectioning for slide preparation and imaging with fluorescence microscopy.

Deep phenotyping can reveal how genetics, environment and stochasticity affect the development, physiology and behavior of an organism. In this Review, Dhaval S. Patel, Nan Xu and Hang Lu outline the technological and analytical developments that have enabled deep-phenotyping studies in Caenorhabditis elegans.

In this study, Åhlgren and Voikar demonstrate that C57BL/6N and C57BL/6J mice from different vendors show substantial behavioral differences, although they are derived from the same strain. These results suggest that different mouse strains and substrains should be included in experiments to address the lack of reproducibility.

Treatment for bacterial sepsis remains limited beyond the use of antibiotics. Lingye Chen, Karen Welty-Wolf, and Bryan Kraft review nonhuman primate models of sepsis and highlight their advantages and limitations compared to other preclinical models.

In this article, Dr. Nancy Moran and coworkers present the use of honey bees as models for gastrointestinal research. They compare and contrast the honey bee with humans and other insects in order to present a balanced perspective of the model.

Authors present the Alliance of Genome Resources Database as an integration of data from six different model species. Current implementation of the database and future challenges are discussed.

Rehabilitation of tumor-afflicted sea turtles, and their utilization as a natural model for human and wildlife cancers.

Human cancer is a disease of cooperating genetic events that is complex to model in vivo. A new study combines somatic base editing with a mouse model of breast cancer, demonstrating the potential to rapidly investigate the function of disease-specific point mutations.