Volume 29 Issue 1-2, February 2022
Cover Credit:Downregulation of Choline-Acetyltransferase in cholinergic interneurons by lentivirus mediated RNAi is linearly correlated with co-expression of a chemogenetic reporter.
RNA interference (RNAi) has important potential for gene-therapy application, such as downregulating neurotransmitter signaling in neuronal circuits. For use of such technologies in humans, it will be necessary to control and monitor such downregulation. In this issue of Gene Therapy,the paper by Lerchner et al. describes a method of potentially monitoring such downregulation in vivo. The image shows viral expression of RNAi and a chemogenetic reporter (green) and remaining expression of Choline Acetyltransferase (red) are inversely correlated in transduced striatal interneurons.
