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  • Original Article
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Carbohydrates, glycemic index and diabetes mellitus

Effects of a novel propionate-rich sourdough bread on appetite and food intake

European Journal of Clinical Nutrition volume 66, pages 789–794 (2012)Cite this article

Subjects

Abstract

Background:

There is evidence linking oral propionate to a reduction in food intake, which could confer functional food properties in the fight against obesity. However, propionate is typically volatile with a pungent smell and taste and so incorporating into foods naturally, at levels acceptable to the consumer is a novel approach.

Subjects/methods:

Twenty healthy, young, normal weight unrestrained eaters underwent an acute feeding study using a palatable sourdough and an identical control bread of a similar palatability, in a randomized cross-over balanced design for the assessment of appetite and energy intake.

Results:

No difference in energy intake of an ad libitum test meal, 180 min after the bread-based breakfast or in energy and macronutrient intake over the entire 24 h period was found between breads. Visual analogue scale ratings for appetite were not influenced by bread type, except the desire to eat something sweet. Elevated plasma insulin concentrations were observed following the propionate-rich sourdough breakfast (P=0.033 no effects of treatment on postprandial glycaemia were found.

Conclusions:

These findings suggest propionate-rich sourdough bread does not influence appetite and food intake unlike larger doses of the food preservative N-propionate.

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References

  1. Foresight. Tackling obesities: future choices – Project report: Government Office for Science 2007. 2007 Report No., 2nd ed.

  2. Food Standards Agency. National Diet and Nutrition Survey Headline results from Year 1 of the Rolling Programme (2008/2009). HMSO2010: Norwich. 2008/2009.

  3. Al-Lahham SH, Peppelenbosch MP, Roelofsen H, Vonk RJ, Venema K . Biological effects of propionic acid in humans; metabolism, potential applications and underlying mechanisms. Biochim Biophys Acta 2010; 1801, 1175–1183.

    Article  CAS  PubMed  Google Scholar 

  4. Cummings JH, Pomare EW, Branch WJ, Naylor CP, Macfarlane GT . Short chain fatty acids in human large intestine, portal, hepatic and venous blood. Gut 1987; 28, 1221–1227.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Liljeberg HGM, Bjorck IME . Delayed gastric emptying rate as a potential mechanism for lowered glycemia after eating sourdough bread: studies in humans and rats using test products with added organic acids or an organic salt. Am J Clin Nutr 1996; 64, 886–893.

    Article  CAS  PubMed  Google Scholar 

  6. Liljeberg HGM, Lonner CH, Bjorck IME . Sourdough fermentation or addition of organic acids or corresponding salts to bread improves nutritional properties of starch in healthy humans. J Nutr 1995; 125, 1503–1511.

    CAS  PubMed  Google Scholar 

  7. Todesco T, Rao AV, Bosello O, Jenkins DJA . Propionate lowers blood glucose and alters lipid metabolism in healthy subjects. Am J Clin Nutr 1991; 54, 860–865.

    Article  CAS  PubMed  Google Scholar 

  8. Darwiche G, Ostman EM, Liljeberg HGM, Kallinen N, Bjorgell O, Bjorck IME et al. Measurements of the gastric emptying rate by use of ultrasonography: studies in humans using bread with added sodium propionate. Am J Clin Nutr 2001; 74, 254–258.

    Article  CAS  PubMed  Google Scholar 

  9. Ge H, Li X, Weiszmann J, Wang P, Baribault H, Chen JL et al. Activation of G protein-coupled receptor 43 in adipocytes leads to inhibition of lipolysis and suppression of plasma free fatty acids. Endocrinology 2008; 149, 4519–4526.

    Article  CAS  PubMed  Google Scholar 

  10. Hong YH, Nishimura Y, Hishikawa D, Tsuzuki H, Miyahara H, Gotoh C et al. Acetate and propionate short chain fatty acids stimulate adipogenesis via GPCR43. Endocrinology 2005; 146, 5092–5099.

    Article  CAS  PubMed  Google Scholar 

  11. Karaki S, Tazoe H, Hayashi H, Kashiwabara H, Tooyama K, Suzuki Y et al. Expression of the short-chain fatty acid receptor, GPR43, in the human colon. J Mol Histol 2008; 39, 135–142.

    Article  CAS  PubMed  Google Scholar 

  12. Tazoe H, Otomo Y, Kaji I, Tanaka R, Karaki SI, Kuwahara A . Roles of short-chain fatty acids receptors, GPR41 and GPR43 on colonic functions. J Physiol Pharmacol 2008; 59, 251–262.

    PubMed  Google Scholar 

  13. Tazoe H, Otomo Y, Karaki S, Kato I, Fukami Y, Terasaki M et al. Expression of short-chain fatty acid receptor GPR41 in the human colon. Biomed Res 2009; 30, 149–156.

    Article  CAS  PubMed  Google Scholar 

  14. Brown AJ, Goldsworthy SM, Barnes AA, Eilert MM, Tcheang L, Daniels D et al. The Orphan G protein-coupled receptors GPR41 and GPR43 are activated by propionate and other short chain carboxylic acids. J Biol Chem 2003; 278, 11312–11319.

    Article  CAS  PubMed  Google Scholar 

  15. Le Poul E, Loison C, Struyf S, Springael JY, Lannoy V, Decobecq ME et al. Functional characterization of human receptors for short chain fatty acids and their role in polymorphonuclear cell activation. J Biol Chem 2003; 278, 25481–15489.

    Article  CAS  PubMed  Google Scholar 

  16. Xiong Y, Miyamoto N, Shibata K, Valasek MA, Motoike T, Kedzierski RM et al. Short-chain fatty acids stimulate leptin production in adipocytes through the G protein-coupled receptor GPR41. Proc Natl Acad Sci USA 2004; 101, 1045–1050.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  17. Mepham B . Food additives: an ethical evaluation. Br Med Bull 2011; 99, 7–23.

    Article  PubMed  Google Scholar 

  18. Frost GS, Brynes AE, Dhillo WS, Bloom SR, McBurney MI . The effects of fiber enrichment of pasta and fat content on gastric emptying, GLP-1, glucose, and insulin responses to a meal. Eur J Clin Nutr 2003; 57, 293–298.

    Article  CAS  PubMed  Google Scholar 

  19. Darzi J, Frost GS, Robertson MD . Do SCFA have a role in appetite regulation? Proc Nutr Soc 2011; 70, 119–128.

    Article  CAS  PubMed  Google Scholar 

  20. Maioli M, Pes GM, Sanna M, Cherchi S, Dettori M, Manca E et al. Sourdough-leavened bread improves postprandial glucose and insulin plasma levels in subjects with impaired glucose tolerance. Acta Diabetol 2008; 45, 91–96.

    Article  CAS  PubMed  Google Scholar 

  21. Najjar AM, Parsons PM, Duncan AM, Robinson LE, Yada RY, Graham TE . The acute impact of ingestion of breads of varying composition on blood glucose, insulin and incretins following first and second meals. Br J Nutr 2009; 101, 391–398.

    Article  CAS  PubMed  Google Scholar 

  22. International Organisation for Standardisation. Sensory analysis - Guidelines for the use of quantitative response scales BS ISO 4121:20032003. 2003.

  23. International Organisation for Standardisation. Sensory analysis - Methodology - Triangle test BS ISO 4120:20042004. 2004.

  24. International Organisation for Standardisation. Sensory analysis - Methodology - General guidance BS ISO 6658:20052005. 2005.

  25. International Organisation for Standardisation. Sensory analysis - Methodology - Paired comparison test BS ISO 5495:20052005. 2005.

  26. International Organisation for Standardisation. Sensory analysis - Methodology - Ranking BS ISO 8587:20062006. 2006.

  27. van Strien T, Frijters JER, Bergers GPA, Defares PB . The Dutch Eating Behavior Questionnaire (DEBQ) for assessment of restrained, emotional, and external eating behavior. Int J Eat Disord 1986; 5, 295–315.

    Article  Google Scholar 

  28. Flint A, Raben A, Blundell JE, Astrup A . Reproducibility, power and validity of visual analogue scales in assessment of appetite sensations in single test meal studies. Int J Obes Relat Metab Disord 2000; 24, 38–48.

    Article  CAS  PubMed  Google Scholar 

  29. Hill AJ, Blundell JE . Nutrients and behaviour: research strategies for the investigation of taste characteristics, food preferences, hunger sensations and eating patterns in man. J Psychiatr Res 1982; 17, 203–212.

    Article  PubMed  Google Scholar 

  30. Bryant M, Truesdale KP, Dye L . Modest changes in dietary intake accross the menstrual cycle: implications for food intake research. Br J Nutr 2006; 96, 888–894.

    Article  CAS  PubMed  Google Scholar 

  31. Bodinham CL, Frost GS, Robertson MD . Acute ingestion of resistant starch reduces food intake in healthy adults. Br J Nutr 2010; 103, 917–922.

    Article  CAS  PubMed  Google Scholar 

  32. Caumo A, Bergman RN, Cobelli C . Insulin sensitivity from meal tolerance tests in normal subjects: a minimal model index. J Clin Endocrinol Metab 2000; 85, 4396–4402.

    Article  CAS  PubMed  Google Scholar 

  33. Bellisle F, Louis-Sylvestre J, Demozay F, Blazy D, Le Magnen J . Cephalic phase of insulin secretion and food stimulation in humans: a new perspective. Am J Physiol 1985; 249, E639–EE45.

    CAS  PubMed  Google Scholar 

  34. Just T, Pau HW, Engel U, Hummel T . Cephalic phase insulin release in healthy humans after taste stimulation? Appetite 2008; 51, 622–627.

    Article  CAS  PubMed  Google Scholar 

  35. Robertson MD, Jackson KG, Williams CM, Fielding BA, Frayn KN . Prolonged effects of modified sham feeding on energy substrate mobilization. Am J Clin Nutr 2001; 73, 111–117.

    Article  CAS  PubMed  Google Scholar 

  36. Teff KL, Engelman K . Oral sensory stimulation improves glucose tolerance in humans: effects on insulin, C-peptide, and glucagon. Am J Physiol 1996; 270, R1371–R13R9.

    CAS  PubMed  Google Scholar 

  37. Feldman M, Richardson CT . Role of thought, sight, smell, and taste of food in the cephalic phase of gastric acid secretion in humans. Gastroenterology 1986; 90, 428–433.

    Article  CAS  PubMed  Google Scholar 

  38. Pavlov IP . The Work of the Digestive Glands. Charles Griffin Co. Ltd.: London, 1902.

    Google Scholar 

  39. Heath RB, Jones R, Frayn KN, Robertson MD . Vagal stimulation exaggerates the inhibitory ghrelin response to oral fat in humans. J Endocrinol 2004; 180, 273–281.

    Article  CAS  PubMed  Google Scholar 

  40. Pouderoux P, Veyrac M, Michel H . Sham feeding disrupts phase III of the duodenal migrating motor complex in humans. Neurogastroenterol Motil 1995; 7, 139–144.

    Article  CAS  PubMed  Google Scholar 

  41. De Graaf C, De Jong LS, Lambers AC . Palatability affects satiation but not satiety. Physiol Behav 1999; 66, 681–688.

    Article  CAS  PubMed  Google Scholar 

  42. Yeomans MR . The role of palatability in control of human appetite: Implications for understanding and treating obesity: appetite and body weight. In: Kirkham TC, Cooper SJ (eds). Appetite and Bodyweight Regulation: Integrative Systems and the Development of Anti-obesity Drugs. Academic Press: New York, 2007, pp 247-269.

    Google Scholar 

  43. Nisbett RE . Taste, deprivation, and weight determinants of eating behavior. J Pers Soc Psychol 1968; 10, 107–116.

    Article  CAS  PubMed  Google Scholar 

  44. Wicks D, Wright J, Rayment P, Spiller R . Impact of bitter taste on gastric motility. Eur J Gastroenterol Hepatol 2005; 17, 961–965.

    Article  PubMed  Google Scholar 

  45. Wolever TM, Spadafora P, Eshuis H . Interaction between colonic acetate and propionate in humans. Am J Clin Nutr 1991; 53, 681–687.

    Article  CAS  PubMed  Google Scholar 

  46. Allen MS, Bradford BJ, Oba M . Board Invited Review: the hepatic oxidation theory of the control of feed intake and its application to ruminants. J Anim Sci 2009; 87, 3317–3334.

    Article  CAS  PubMed  Google Scholar 

  47. Diplock AT, Aggett PJ, Ashwell M, Bornet F, Fern EB, Roberfroid MB . Scientific concepts of functional food in Europe: consensus document. Br J Nutr 1999; 81, S1–S28.

    Article  CAS  Google Scholar 

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Acknowledgements

We are grateful to all the volunteers who participated in the study and also to Drs Shelagh Hampton and John Wright for medical assistance. JD was involved in designing and conducting the research, analysed the data and wrote the paper. MDR was involved in designing the research and refining the paper. GSF was involved in designing the research, refining the paper. All authors read and approved the final manuscript. JD was supported by an educational fellowship from Premier Foods and bread test products were manufactured and supplied by Holgran Ltd (Burton-on-Trent, UK).

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Authors and Affiliations

  1. Diabetes and Metabolic Medicine, Postgraduate Medical School, University of Surrey, Guildford, UK

    J Darzi & M D Robertson

  2. Division of Diabetes, Nutrition and Dietetics Research Group, Endocrinology and Metabolism, Faculty of Medicine, Imperial College London, London, UK

    G S Frost

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  1. J Darzi
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Correspondence to M D Robertson.

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Darzi, J., Frost, G. & Robertson, M. Effects of a novel propionate-rich sourdough bread on appetite and food intake. Eur J Clin Nutr 66, 789–794 (2012). https://doi.org/10.1038/ejcn.2012.1

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