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Alpha-tocopherol supplementation prevents the exercise-induced reduction of serum paraoxonase 1/arylesterase activities in healthy individuals

European Journal of Clinical Nutrition volume 63, pages 215–221 (2009)Cite this article

Abstract

Objective:

To investigate PON 1/Aryl activities in basketball players with or without α-T supplementation pre- and post-training. Vitamin E (α-tocopherol, α-T) reduces lipid peroxidation. Paraoxonase 1/arylesterase (PON 1/Aryl) activities are closely related to oxidation and atherogenesis.

Subject/Methods:

Blood was obtained from 10 players pre- (group A), post-exercise (group B) and after 1 month on α-T (200 mg per 24 h orally) supplementation pre- (group C) and post-exercise (group D). Lactate, pyruvate, muscle enzyme activities, creatine kinase, lactate dehydrogenase and total antioxidant status (TAS) were measured with commercial kits. Catecholamines and α-T were determined with high-performance liquid chromatography methods and PON 1/Aryl activities spectrophotometrically.

Results:

Lactate, pyruvate, muscle enzyme activities and catecholamines were increased (P<0.001) in all groups post-training. Alpha-T levels remained unaltered pre- vs post-exercise. TAS was decreased in all the groups post training. PON 1/Aryl activities were significantly decreased post-exercise (group B) (PON1: 65±12 U min−1 ml−1, Aryl: 58±14 KU min−1 ml−1) as compared to those pre-exercise (group A) (PON1: 142±16 U min−1 ml−1, Aryl: 114±12 KU min−1 ml−1, P<0.001). In contrast, the studied enzyme activities remained practically unaltered after α-T supplementation pre- vs post-training. Both enzyme activities positively correlated to TAS (r=0.60, P<0.001).

Conclusions:

Alpha-T supplementation may result in protection of the enzyme PON 1/Aryl activities from free radical production.

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Acknowledgements

This work was funded by the University of Athens and the Institute of Child Health. We thank Mrs Kalliopi Tassopoulou for the careful typing of this paper and the medical students Zois Mellios and Alexios Mentis for their significant assistance.

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Authors and Affiliations

  1. Department of Experimental Physiology, Medical School, University of Athens, Athens, Greece

    S Tsakiris, T Parthimos, T Tsakiris & C Bakogiannis

  2. Department of Medical Laboratories, Technological and Educational Institution of Athens (TEI-A), Athens, Greece

    G A Karikas

  3. Institute of Child Health, Research Center, ‘Aghia Sophia’ Children's Hospital, Athens, Greece

    K H Schulpis

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  1. S Tsakiris
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Correspondence to S Tsakiris.

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Tsakiris, S., Karikas, G., Parthimos, T. et al. Alpha-tocopherol supplementation prevents the exercise-induced reduction of serum paraoxonase 1/arylesterase activities in healthy individuals. Eur J Clin Nutr 63, 215–221 (2009). https://doi.org/10.1038/sj.ejcn.1602918

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