Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Perspective
  • Published:

Structural genomics of RNA

Nature Structural Biology volume 7, pages 954–956 (2000)Cite this article

Abstract

A detailed understanding of the functions and interactions of biological macromolecules requires knowledge of their molecular structures. Structural genomics, the systematic determination of all macromolecular structures represented in a genome, is focused at present exclusively on proteins. It is clear, however, that RNA molecules play a variety of significant roles in cells, including protein synthesis and targeting, many forms of RNA processing and splicing, RNA editing and modification, and chromosome end maintenance. To comprehensively understand the biology of a cell, it will ultimately be necessary to know the identity of all encoded RNAs, the molecules with which they interact and the molecular structures of these complexes. This report focuses on the feasibility of structural genomics of RNA, approaches to determining RNA structures and the potential usefulness of an RNA structural database for both predicting folds and deciphering biological functions of RNA molecules.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Figure 1: Secondary structure of the group I class of self-splicing introns.
Figure 2: Solution and crystal structures of the 50-nucleotide domain IV of signal recognition particle 4.5S RNA.

Similar content being viewed by others

References

  1. Mitchell, J.R., Cheng, J. & Collins, K. Mol. Cell. Biol. 19, 567– 576 (1999).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Moore, P.B. Annu. Rev. Biochem. 68, 287–300 (1999).

    Article  CAS  PubMed  Google Scholar 

  3. Leontis, N.B. & Westhof, E. Quart. Rev. Biophys. 31, 399–455 (1998).

    Article  CAS  Google Scholar 

  4. Schmitz, U. et al. RNA 5, 1419–1429 (1999).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Batey, R.T., Rambo, R.P., Lucast, L., Rha, B. & Doudna, J.A. Science 287, 1232– 1239 (2000).

    Article  CAS  PubMed  Google Scholar 

  6. Jovine, L. et al. Structure 8, 527–540 (2000).

    Article  CAS  PubMed  Google Scholar 

  7. Ban, N., Nissen, P., Hansen, J., Moore, P.B. & Steitz, T.A. Science 289, 905– 920 (2000).

    Article  CAS  PubMed  Google Scholar 

  8. Ferre-D'Amare, A.R., Zhou, K. & Doudna, J.A. Nature 395, 567– 574 (1998).

    Article  CAS  PubMed  Google Scholar 

  9. Cate, J.H. et al. Science 273, 1678–1685 (1996).

    Article  CAS  PubMed  Google Scholar 

  10. Butcher, S.E., Dieckmann, T. & Feigon, J. EMBO J. 16, 7490– 7299 (1997).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Bourdeau, V., Ferbeyre, G., Pageau, M., Paquin, B. & Cedergren R. Nucleic Acids. Res. 27, 4457– 4467 (1999).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Lowe, T.M. & Eddy, S.R. Science 283, 1168–1171 (1999).

    Article  CAS  PubMed  Google Scholar 

  13. Omer, A.D. et al. Science 288, 517–522 (2000).

    Article  CAS  PubMed  Google Scholar 

  14. Latham, J.A. & Cech, T.R. Science 245, 276–282 (1989).

    Article  CAS  PubMed  Google Scholar 

  15. Christian, E.L. & Yarus, M. J. Mol. Biol. 228, 743–758 (1992).

    Article  CAS  PubMed  Google Scholar 

  16. Harris, M.E., Kazantsev, A.V., Chen, J.L. & Pace, N.R. RNA 3, 561–576 ( 1997).

    CAS  PubMed  PubMed Central  Google Scholar 

  17. Ryder, S.P., Ortoleva-Donnelly, L., Kosek, A.B. & Strobel, S.A. Methods Enzymol. 317, 92–109 (2000).

    Article  CAS  PubMed  Google Scholar 

  18. Stowell, M.H., Miyazawa, A. & Unwin, N. Curr. Opin. Struct. Biol. 8, 595–600 (1998).

    Article  CAS  PubMed  Google Scholar 

  19. Ferre-D'Amare, A.R. & Doudna, J.A. Nucleic Acids Res. 24, 977–978 ( 1996).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  20. Doudna, J.A., Grosshans, C., Gooding, A. & Kundrot, C.E. Proc. Natl. Acad. Sci. USA 90, 7829– 7833 (1993).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  21. Scott, W.G. et al. J. Mol. Biol. 250, 327– 332 (1995).

    Article  CAS  PubMed  Google Scholar 

  22. Golden, B.L., Podell, E.R., Gooding, A.R. & Cech, T.R. J. Mol. Biol. 270, 711–723 (1997).

    Article  CAS  PubMed  Google Scholar 

  23. Ferre-D'Amare, A.R., Zhou, K., Doudna, J.A. J. Mol. Biol. 279, 621–631 (1998).

    Article  CAS  PubMed  Google Scholar 

  24. Ferre-D'Amare, A.R. & Doudna, J.A. J. Mol. Biol. 295, 541–556 ( 2000).

    Article  CAS  PubMed  Google Scholar 

  25. Burley, S.K. et al. Nature Genet. 23, 151– 157 (1999).

    Article  CAS  PubMed  Google Scholar 

  26. Michel, F. & Westhof, E. J. Mol. Biol. 216, 585–610 (1990).

    Article  CAS  PubMed  Google Scholar 

  27. Golden, B.L., Gooding, A.R., Podell, E.R. & Cech, T.R. Science 282, 259–264 ( 1998).

    Article  CAS  PubMed  Google Scholar 

  28. Tanner, N.K. et al. Curr. Biol. 4, 488– 498 (1994).

    Article  CAS  PubMed  Google Scholar 

  29. Schluenzen, F. et al. Cell 102, 615 ( 2000).

    Article  CAS  PubMed  Google Scholar 

  30. Wimberly, B.T. et al. Nature 407, 327–339 (2000).

    Article  CAS  PubMed  Google Scholar 

  31. Carter, A.P. et al. Nature 407, 340–348 (2000).

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Howard Hughes Medical Institute and Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, 06520, Connecticut, USA

    Jennifer A. Doudna

Authors
  1. Jennifer A. Doudna
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Doudna, J. Structural genomics of RNA. Nat Struct Mol Biol 7 (Suppl 11), 954–956 (2000). https://doi.org/10.1038/80729

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1038/80729

This article is cited by

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing