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  • Article
    | Open Access

    Brinton, Uauy and colleagues utilize genomic data from the 10+ Wheat Genome Project to develop a useful tool for studying and generating new wheat cultivars. This framework uses advanced exploitation of wheat haplotypes to bring newfound precision and efficiency to wheat breeding.

    • Jemima Brinton
    • , Ricardo H. Ramirez-Gonzalez
    • , James Simmonds
    • , Luzie Wingen
    • , Simon Orford
    • , Simon Griffiths
    • , Georg Haberer
    • , Manuel Spannagl
    • , Sean Walkowiak
    • , Curtis Pozniak
    •  & Cristobal Uauy
  • Article
    | Open Access

    Didriksen, Nawaz, et al. identify three novel genetic risk variants for restless legs syndrome and confirm 19 out of 20 previously reported variants through a genome-wide association meta-analysis including nearly half a million individuals. Using expression QTL analysis, they also find that a subset of these loci may have a causal effect on nearby gene expression.

    • Maria Didriksen
    • , Muhammad Sulaman Nawaz
    • , Joseph Dowsett
    • , Steven Bell
    • , Christian Erikstrup
    • , Ole B. Pedersen
    • , Erik Sørensen
    • , Poul J. Jennum
    • , Kristoffer S. Burgdorf
    • , Brendan Burchell
    • , Adam S. Butterworth
    • , Nicole Soranzo
    • , David B. Rye
    • , Lynn Marie Trotti
    • , Prabhjyot Saini
    • , Lilja Stefansdottir
    • , Sigurdur H. Magnusson
    • , Gudmar Thorleifsson
    • , Thordur Sigmundsson
    • , Albert P. Sigurdsson
    • , Katja Van Den Hurk
    • , Franke Quee
    • , Michael W. T. Tanck
    • , Willem H. Ouwehand
    • , David J. Roberts
    • , Eric J. Earley
    • , Michael P. Busch
    • , Alan E. Mast
    • , Grier P. Page
    • , John Danesh
    • , Emanuele Di Angelantonio
    • , Hreinn Stefansson
    • , Henrik Ullum
    •  & Kari Stefansson
  • Article
    | Open Access

    Michael Donovan et al. examine well-preserved remains of diverse insect-feeding and fungal damage on Patagonian fossil and extant material of the conifer Agathis. They report a suite of blotch mines, galls, scale-insect covers, and rust fungus that re-occur on the same host genus through time and space, showing the persistence of ecological guilds and possible host-tracking across major plate movements since the late Mesozoic.

    • Michael P. Donovan
    • , Peter Wilf
    • , Ari Iglesias
    • , N. Rubén Cúneo
    •  & Conrad C. Labandeira
  • Article
    | Open Access

    By integrating transcriptome, regulatory element and chromosome topology profiles, Schwartz et al identify redistribution of p53 and CTCF genomic binding to be associated with transcriptional and phenotypical changes during Ras-induced transformation of mammary epithelial cells.

    • Michal Schwartz
    • , Avital Sarusi Portugez
    • , Bracha Zukerman Attia
    • , Miriam Tannenbaum
    • , Leslie Cohen
    • , Olga Loza
    • , Emily Chase
    • , Yousef Turman
    • , Tommy Kaplan
    • , Zaidoun Salah
    •  & Ofir Hakim
  • Article
    | Open Access

    Pinotsis and Miller present a simplified neural mass model for estimating the laminar dynamics that contribute to non-invasively recorded time frequency data. Using two independent MEG datasets, they give evidence for deep cortical layers contributing to inter-individual variability in visually induced oscillations. Their study links non-invasive brain imaging data, laminar dynamics and top-down control.

    • Dimitris A. Pinotsis
    •  & Earl K. Miller
  • Article
    | Open Access

    Using genetic code expansion, Zhao, Shi et al. generate light-sensitive tyrosine analogues to obtain insights into the activation of the NGF receptor, TrkA. They identify light-sensitive and NGF-insensitive phosphorylation sites, validating the approach and providing insights into TrkA signaling

    • Shu Zhao
    • , Jia Shi
    • , Guohua Yu
    • , Dali Li
    • , Meng Wang
    • , Chonggang Yuan
    • , Huihui Zhou
    • , Amirabbas Parizadeh
    • , Zhenlin Li
    • , Min-Xin Guan
    •  & Shixin Ye
  • Article
    | Open Access

    Pitzalis et al. use replicative RNAseq, small RNA (sRNA)seq, and parallel analysis of RNA ends (PARE)seq analysis to identify networks of sRNAs-guided post-transcriptional regulation within local Turnip mosaic virus infection sites. This study provides insights into the complex regulatory networking at the plantvirus interface within cells undergoing early stages of infection.

    • Nicolas Pitzalis
    • , Khalid Amari
    • , Stéfanie Graindorge
    • , David Pflieger
    • , Livia Donaire
    • , Michael Wassenegger
    • , César Llave
    •  & Manfred Heinlein
  • Article
    | Open Access

    Wright et al. report the first structure of a human TRPC1/4/5 channel in complex with a xanthine-based TRPC5 inhibitor Pico145. They find that Pico145 binds to a conserved lipid binding site of TRPC5, where it displaces a phospholipid. This study provides insights into the mechanism-of-action of xanthine-based TRPC1/4/5 modulators.

    • David J. Wright
    • , Katie J. Simmons
    • , Rachel M. Johnson
    • , David J. Beech
    • , Stephen P. Muench
    •  & Robin S. Bon
  • Article
    | Open Access

    Pranam Chatterjee et al. present a novel computational platform for engineering peptide fusions that bind to the SARS-CoV-2 spike protein and tag it for proteasomal degradation. They experimentally validate an optimal variant in human cells, showing that it inhibits production of infection-competent virus.

    • Pranam Chatterjee
    • , Manvitha Ponnapati
    • , Christian Kramme
    • , Alexandru M. Plesa
    • , George M. Church
    •  & Joseph M. Jacobson
  • Article
    | Open Access

    Morahan et al. investigate inhibitors of human mitotic kinases in P. falciparum and show a resistance mechanism to the drug Hesperadin through an epistatic interaction between the PfArk1 and PfNek1 kinases. This study demonstrates that existing inhibitors can be used to validate additional essential and druggable kinase functions in the parasite.

    • Belinda J. Morahan
    • , Clarissa Abrie
    • , Keith Al-Hasani
    • , Mitchell B. Batty
    • , Victoria Corey
    • , Anne N. Cowell
    • , Jandeli Niemand
    • , Elizabeth A. Winzeler
    • , Lyn-Marie Birkholtz
    • , Christian Doerig
    •  & Jose F. Garcia-Bustos
  • Article
    | Open Access

    Joseph Angeloni et al. show that Caenorhabditis elegans can detect and are attracted to multiple signal molecules produced by the bacterium Vibrio cholerae, specifically the 3′,5′-cyclic diguanylate (c-di-GMP), even though this bacterium kills the host at a high rate. This study reveals how bacterial c-di-GMP may serve as a cue for C. elegans that they can use to detect food or alternatively, impair their immune response, which could facilitate bacterial invasion and survival.

    • Joseph Angeloni
    • , Yuqing Dong
    • , Zeneng Wang
    •  & Min Cao
  • Article
    | Open Access

    Tindell et al. identify glial granules in the adult Drosophila brain. The glial granules contain polypeptides known to function in germ cells, including Tudor. They find that Tudor in glial granules silences transposable elements and regulates the biogenesis of piRNA. This study uncovers previously unknown glial granules that regulate brain transcriptome.

    • Samuel J. Tindell
    • , Eric C. Rouchka
    •  & Alexey L. Arkov
  • Article
    | Open Access

    Nega et al. shed light on the interplay of the two domain enzymes of the major autolysin, AmiA and GlcA, in S. aureus for peptidoglycan hydrolysis during bacterial cell division. They show that GlcA requires the prior removal of the stem peptide by AmiA for its activity and that GlcA is not an endo-enzyme as previously thought, but an exo-enzyme that chops down the glycan backbone to disaccharides independent of its O-acetylation modification.

    • Mulugeta Nega
    • , Paula Maria Tribelli
    • , Katharina Hipp
    • , Mark Stahl
    •  & Friedrich Götz
  • Article
    | Open Access

    Christian Kappel et al. examine the genetic composition of fairy circles, regular circular patterns of grasses in the Namib Desert, using ddRAD-seq. They find that these grasses are made up of multiple unrelated genets rather than genetically identical grasses, suggesting non-clonality.

    • Christian Kappel
    • , Nicola Illing
    • , Cuong Nguyen Huu
    • , Nichole N. Barger
    • , Michael D. Cramer
    • , Michael Lenhard
    •  & Jeremy J. Midgley
  • Article
    | Open Access

    Ke Shi et al. perform a structural and biochemical characterization of the complex formed between Spackle, an early gene product of T4 and the gp5 lysozyme located in the tail of T4, thereby revealing a new mode of inhibition of the well-known lysozyme enzyme. This study provides structural insight into the phenomenon of superinfection exclusion, phenomenon by which bacterial cells infected with a T4 phage become resistant to secondary infections by the same or closely related phages.

    • Ke Shi
    • , Justin T. Oakland
    • , Fredy Kurniawan
    • , Nicholas H. Moeller
    • , Surajit Banerjee
    •  & Hideki Aihara
  • Article
    | Open Access

    Sonali Setua et al. identify a small molecule which is capable of disrupting the interaction between the plasmodium-derived protein UIS3 and the host protein LC3. Disruption of this interaction by this small molecule triggers parasite elimination from host cells in an autophagy-dependent manner, without affecting the cells’ normal autophagy pathway.

    • Sonali Setua
    • , Francisco J. Enguita
    • , Ângelo Ferreira Chora
    • , Harish Ranga-prasad
    • , Aparajita Lahree
    • , Sofia Marques
    • , Varadharajan Sundaramurthy
    •  & Maria M. Mota
  • Article
    | Open Access

    Ana Chucair-Elliott, Sarah Ocaňas et al. present a NuTRAP approach for simultaneous analysis of transcript expression and DNA modifications in two specific mouse brain cell types, astrocytes and microglia. They further apply this approach to identify molecular changes in microglia following LPS treatment and identify both transcriptomic and epigenomic alterations not apparent in tissue-level analyses.

    • Ana J. Chucair-Elliott
    • , Sarah R. Ocañas
    • , David R. Stanford
    • , Victor A. Ansere
    • , Kyla B. Buettner
    • , Hunter Porter
    • , Nicole L. Eliason
    • , Justin J. Reid
    • , Amanda L. Sharpe
    • , Michael B. Stout
    • , Michael J. Beckstead
    • , Benjamin F. Miller
    • , Arlan Richardson
    •  & Willard M. Freeman
  • Article
    | Open Access

    Sarah E. Donohue et al. characterize the spatiotemporal propagation of target and distractor processing in the human visual cortex. They show that these signals propagate in parallel as fast and slow sweeps from higher to lower hierarchical levels, and that the fast target processing signal can bypass intermediate levels correlating with behavioral performance.

    • Sarah E. Donohue
    • , Mircea A. Schoenfeld
    •  & Jens-Max Hopf
  • Article
    | Open Access

    AhYoung, Eckard et al. show that the expression of Neutrophil Serine Protease 4 (NSP4) during the early stages of mast cell development regulates the levels of histamine and serotonin in mast cell granules. This study reveals an important physiological function of NSP4 in mast cell-mediated vascular leakage in mice, establishing NSP4 as a potential therapeutic target for mast cell-dependent immune disorders.

    • Andrew P. AhYoung
    • , Sterling C. Eckard
    • , Alvin Gogineni
    • , Hongkang Xi
    • , S. Jack Lin
    • , Stefan Gerhardy
    • , Christian Cox
    • , Qui T. Phung
    • , Jason A. Hackney
    • , Anand Kumar Katakam
    • , Mike Reichelt
    • , Patrick Caplazi
    • , Paolo Manzanillo
    • , Juan Zhang
    • , Merone Roose-Girma
    • , Lucinda W. Tam
    • , Robert J. Newman
    • , Aditya Murthy
    • , Robby M. Weimer
    • , Jennie R. Lill
    • , Wyne P. Lee
    • , Michele Grimbaldeston
    • , Daniel Kirchhofer
    •  & Menno van Lookeren Campagne
  • Article
    | Open Access

    Neil Burns et al. use a continuous-surface otolith microchemistry approach to geolocate whiting across the west coast of the UK and show connectivity across existing stock boundaries, highlighting the importance of the Firth of Clyde nursery area. This study is important for fisheries management and will have an influence on methods used to infer connectivity from otolith microchemistry.

    • Neil M. Burns
    • , Charlotte R. Hopkins
    • , David M. Bailey
    •  & Peter J. Wright
  • Article
    | Open Access

    Using hyperpolarized 13C magnetic resonance imaging (MRI), Timm et al. show that a change in oxidative mitochondrial carbohydrate metabolism precedes cardiac decline in a rat model of heart failure induced by doxorubicin (DOX), a widely used chemotherapeutic agent. This study suggests the possibility of using hyperpolarized MRI to test cancer patients receiving DOX for early signs of cardiotoxicity.

    • Kerstin N. Timm
    • , Charith Perera
    • , Vicky Ball
    • , John A. Henry
    • , Jack J. Miller
    • , Matthew Kerr
    • , James A. West
    • , Eshita Sharma
    • , John Broxholme
    • , Angela Logan
    • , Dragana Savic
    • , Michael S. Dodd
    • , Julian L. Griffin
    • , Michael P. Murphy
    • , Lisa C. Heather
    •  & Damian J. Tyler
  • Article
    | Open Access

    Neville et al. investigate how Streptococcus pneumoniae mitigates metal ion stress. Despite cadmium induced dysregulation of central carbon metabolism and lipid membrane homeostasis, they find that S. pneumoniae can remain viable by selectively utilizing predominately metal-independent cellular pathways. This study provides insights into how bacteria overcome metal ion toxicity.

    • Stephanie L. Neville
    • , Bart A. Eijkelkamp
    • , Amber Lothian
    • , James C. Paton
    • , Blaine R. Roberts
    • , Jason W. Rosch
    •  & Christopher A. McDevitt
  • Article
    | Open Access

    Holman et al. develop a grid-less autonomous adaptive sampling method to explore high-dimensional spatiochemical experimental systems. Their method greatly decreases image acquisition time while improving spatial resolution, and when implemented with FTIR, it outperforms existing standard grid sampling approaches. They further show its utility for a complex biological sample, C. elegans.

    • Elizabeth A. Holman
    • , Yuan-Sheng Fang
    • , Liang Chen
    • , Michael DeWeese
    • , Hoi-Ying N. Holman
    •  & Paul W. Sternberg
  • Article
    | Open Access

    Borer et al. use experimental data and mathematical simulations to show how the expansion behavior of the bacteria P. stutzeri varies when exhibiting different types of trophic interactions. This study offers a unifying explanation for the emergence of patterns attributed to variation in localized growth rates of cells mediated by environmental feedbacks.

    • Benedict Borer
    • , Davide Ciccarese
    • , David Johnson
    •  & Dani Or
  • Article
    | Open Access

    Ishida et al. investigated genome-wide associations between 1068 healthy Japanese adults and their gut microbiota. They find that five loci account for a small variation of the human gut microbiota composition and that six genera are heritable. This study suggests that human gut microbiota composition is likely to be affected by multiple genetic loci.

    • Sachiko Ishida
    • , Kumiko Kato
    • , Masami Tanaka
    • , Toshitaka Odamaki
    • , Ryuichi Kubo
    • , Eri Mitsuyama
    • , Jin-zhong Xiao
    • , Rui Yamaguchi
    • , Satoshi Uematsu
    • , Seiya Imoto
    •  & Satoru Miyano
  • Article
    | Open Access

    Chowdhury Abdulla and Richa Aishwarya et al. report that methamphetamine exposure in mice leads to cardiomyopathy via the signaling receptor Sigmar1. They find that methamphetamine inhibits Sigmar1, which alters mitochondrial function, and show that their findings are consistent with observations in hearts from human methamphetamine users.

    • Chowdhury S. Abdullah
    • , Richa Aishwarya
    • , Shafiul Alam
    • , Mahboob Morshed
    • , Naznin Sultana Remex
    • , Sadia Nitu
    • , Gopi K. Kolluru
    • , James Traylor
    • , Sumitra Miriyala
    • , Manikandan Panchatcharam
    • , Brandon Hartman
    • , Judy King
    • , Mohammad Alfrad Nobel Bhuiyan
    • , Sunitha Chandran
    • , Matthew D. Woolard
    • , Xiuping Yu
    • , Nicholas E. Goeders
    • , Paari Dominic
    • , Connie L. Arnold
    • , Karen Stokes
    • , Christopher G. Kevil
    • , A. Wayne Orr
    •  & Md. Shenuarin Bhuiyan
  • Article
    | Open Access

    Frey and colleagues describe a new fossil shark from the Late Devonian of Morocco in which the specialized jaw articulation is unusually well preserved. Biomechanical modelling reveals that the mandible rolled throughout its movement arc, engaging more of its tooth battery in a clutching bite action.

    • Linda Frey
    • , Michael I. Coates
    • , Kristen Tietjen
    • , Martin Rücklin
    •  & Christian Klug
  • Article
    | Open Access

    Kumar et al. use proteomes of patients with falciparum and vivax malaria and dengue fever, and identify differentially abundant proteins and their associated functions in the different severities and diseases. This study offers mechanistic pathways to malaria severity, and adopts a machine learning approach for predictive proteins for the malarial types and severities.

    • Vipin Kumar
    • , Sandipan Ray
    • , Shalini Aggarwal
    • , Deeptarup Biswas
    • , Manali Jadhav
    • , Radha Yadav
    • , Sanjeev V. Sabnis
    • , Soumaditya Banerjee
    • , Arunansu Talukdar
    • , Sanjay K. Kochar
    • , Suvin Shetty
    • , Kunal Sehgal
    • , Swati Patankar
    •  & Sanjeeva Srivastava
  • Article
    | Open Access

    Gao et al. design a lung targeting nanovesicles made from human neutrophils membrane, and co-loaded inflammatory resolving mediator RvD1 and antibiotic CAZ inside the nanovesicles. These nanovesicles could accelerate the inflammation resolution and release the antibiotics to treat bacterial infections in mice. This approach is useful in developing multifunctional nanoparticle-based therapeutics for lung bacterial infections treatment.

    • Jin Gao
    • , Sihan Wang
    • , Xinyue Dong
    • , Leon G. Leanse
    • , Tianhong Dai
    •  & Zhenjia Wang
  • Article
    | Open Access

    Ito et al. succeed in detecting guanosine tetraphosphate (ppGpp) in measurable levels in metazoan, specifically in Drosophila. They further demonstrate that the ppGpp-specific hydrolase, metazoan SpoT homolog-1 (Mesh1), is necessary, at least in certain conditions, to maintain low ppGpp levels, hence providing insights into the role of Mesh1 as a ppGpp hydrolase in vivo.

    • Doshun Ito
    • , Hinata Kawamura
    • , Akira Oikawa
    • , Yuta Ihara
    • , Toshio Shibata
    • , Nobuhiro Nakamura
    • , Tsunaki Asano
    • , Shun-Ichiro Kawabata
    • , Takashi Suzuki
    •  & Shinji Masuda
  • Article
    | Open Access

    Tao Zhu et al. present UMI-ATAC-seq, an improved ATAC-seq technique that uses unique molecular identifiers to avoid filtering out true independent - but identical - Tn5 insertion events that would otherwise be treated as PCR duplicates. They apply UMI-ATAC-seq to rice panicle samples and show that their approach improves the quantification of chromatin accessibility and footprinting.

    • Tao Zhu
    • , Keyan Liao
    • , Rongfang Zhou
    • , Chunjiao Xia
    •  & Weibo Xie
  • Article
    | Open Access

    Miguel et al. identify a new enzyme belonging to the GDSL lipase-like family that is involved in the final stage of transformation of 5-CQA into 3,5-diCQA. This enzyme is able to realize an efficient transformation by over 60%, making the transformation process a valuable technological tool that can be easily transferred on an industrial scale.

    • Sissi Miguel
    • , Guillaume Legrand
    • , Léonor Duriot
    • , Marianne Delporte
    • , Barbara Menin
    • , Cindy Michel
    • , Alexandre Olry
    • , Gabrielle Chataigné
    • , Aleksander Salwinski
    • , Joakim Bygdell
    • , Dominique Vercaigne
    • , Gunnar Wingsle
    • , Jean Louis Hilbert
    • , Frédéric Bourgaud
    • , Alain Hehn
    •  & David Gagneul
  • Article
    | Open Access

    Berryman et al demonstrate that disaggregated cells can be phenotyped with a high degree of accuracy from bright-field and non-specifically stained microscopy images using a trained convolutional neural network (CNN). This approach allows for the identification of cell types without the need for specific markers.

    • Samuel Berryman
    • , Kerryn Matthews
    • , Jeong Hyun Lee
    • , Simon P. Duffy
    •  & Hongshen Ma
  • Article
    | Open Access

    Kwan et al. show the interaction between the HACS1 SH3 domain and a sequence near the third immunoreceptor tyrosine-based inhibition motif of the Paired immunoglobulin receptor B (PIRB). This study suggests that the HACS1-PIRB partnership has the potential to unite signaling pathways that regulate both immune and neuronal cell fate.

    • Jamie J. Kwan
    • , Sladjana Slavkovic
    • , Michael Piazza
    • , Dingyan Wang
    • , Thorsten Dieckmann
    • , Philip E. Johnson
    • , Xiao-Yan Wen
    •  & Logan W. Donaldson
  • Article
    | Open Access

    Rosanna Sestito et al. report a miR-200b/c-mediated regulatory circuit that drives ovarian cancer metastasis via the endothelin A receptor (ETAR). They show that blockade of ETAR increases miR-200b/c expression and subsequently reduces ZEB1 expression, thereby inhibiting further metastatic progression.

    • Rosanna Sestito
    • , Roberta Cianfrocca
    • , Piera Tocci
    • , Laura Rosanò
    • , Andrea Sacconi
    • , Giovanni Blandino
    •  & Anna Bagnato
  • Article
    | Open Access

    Britney Helling et al. report that cultured bronchial cells from adults with asthma show different gene expression and chromatin accessibility patterns when exposed to rhinovirus than do cells from individuals without asthma. Their data suggest that rhinovirus infection leads to a delayed or elongated activation of inflammatory genes in individuals with asthma compared to those without asthma.

    • Britney A. Helling
    • , Débora R. Sobreira
    • , Grace T. Hansen
    • , Noboru J. Sakabe
    • , Kaixuan Luo
    • , Christine Billstrand
    • , Bharathi Laxman
    • , Raluca I. Nicolae
    • , Dan L. Nicolae
    • , Yury A. Bochkov
    • , James E. Gern
    • , Marcelo A. Nobrega
    • , Steven R. White
    •  & Carole Ober
  • Article
    | Open Access

    Yuan et al. propose an unsupervised deep learning network approach to analyze the dynamics of membrane proteins from the fluorescence intensity traces. The unsupervised nature facilitates training of the system without predefined state number or pre-labelling and can even extract information from noise distribution.

    • Jinghe Yuan
    • , Rong Zhao
    • , Jiachao Xu
    • , Ming Cheng
    • , Zidi Qin
    • , Xiaolong Kou
    •  & Xiaohong Fang
  • Article
    | Open Access

    Albarracín Orio et al. show that an environmental bacterial strain of Bacillus subtilis undergoes heritable phenotypic variation when it interacts with a soil fungal pathogen Setophoma terrestris. This study suggests B. subtilis metabolism may be rewired to increase the production of antifungal compounds during its antagonistic interaction with S. terrestris.

    • Andrea G. Albarracín Orio
    • , Daniel Petras
    • , Romina A. Tobares
    • , Alexander A. Aksenov
    • , Mingxun Wang
    • , Florencia Juncosa
    • , Pamela Sayago
    • , Alejandro J. Moyano
    • , Pieter C. Dorrestein
    •  & Andrea M. Smania
  • Article
    | Open Access

    Ceolin et al. optimise the fluorescent protein mNeonGreen to generate a reporter to measure enhancer expression in living Drosophila embryos. They also find that reduced enhancer–promoter distance or addition of binding sites for the pioneer transcription factor Zelda increases enhancer activity.

    • Stefano Ceolin
    • , Monika Hanf
    • , Marta Bozek
    • , Andrea Ennio Storti
    • , Nicolas Gompel
    • , Ulrich Unnerstall
    • , Christophe Jung
    •  & Ulrike Gaul
  • Article
    | Open Access

    Sylvie Gimenez et al. compare adaptation by copy number variation in two geographically distinct populations of the fall armyworm and find a relationship between copy number variation and insecticide resistance. The authors perform toxicological tests to confirm a role of the CYP gene cluster in insecticide resistance and suggest that evolutionary forces causing insecticide resistance can be independent of host-plant adaptation.

    • Sylvie Gimenez
    • , Heba Abdelgaffar
    • , Gaelle Le Goff
    • , Frédérique Hilliou
    • , Carlos A. Blanco
    • , Sabine Hänniger
    • , Anthony Bretaudeau
    • , Fabrice Legeai
    • , Nicolas Nègre
    • , Juan Luis Jurat-Fuentes
    • , Emmanuelle d’Alençon
    •  & Kiwoong Nam
  • Article
    | Open Access

    Lan et al. make single and triple phosphomimetic mutations in a long flexible loop domain of BCL-2 and show that these mutations reduce structural flexibility and promote ligand access to the groove. Their study indicates that changes in structural flexibility on an unstructured loop can activate an allosteric, otherwise inactive protein.

    • Yu-Jing Lan
    • , Pei-Shan Yeh
    • , Te-Yu Kao
    • , Yuan-Chao Lo
    • , Shih-Che Sue
    • , Yu-Wen Chen
    • , Dennis W. Hwang
    •  & Yun-Wei Chiang
  • Article
    | Open Access

    Sato et al. find that WNK (With No Lysine [K]) acts as a positive regulator of the Wnt signaling pathway by attenuating the interaction between β-Catenin and the Glucose Induce degradation Deficient (GID) complex, and show that a WNK inhibitor also functions as a Wnt inhibitor, suppressing xenograft tumor development in mice. These findings suggest that WNK is a regulator of β-Catenin and a potential therapeutic target

    • Atsushi Sato
    • , Masahiro Shimizu
    • , Toshiyasu Goto
    • , Hiroyuki Masuno
    • , Hiroyuki Kagechika
    • , Nobuyuki Tanaka
    •  & Hiroshi Shibuya
  • Article
    | Open Access

    Rosendo-Pineda et al. discover that N-methyl-D-aspartate receptors (NMDAR) mediate transient calcium entry in mitosis, dependent on phosphorylation of NMDAR by Cyclin B1/CDK1. They also find that phosphorylation-mimicking mutants of NMDAR cause mitotic defects, suggesting that precise control of NMDAR-mediated calcium entry is needed for proper mitosis.

    • Margarita Jacaranda Rosendo-Pineda
    • , Juan Jesus Vicente
    • , Oscar Vivas
    • , Jonathan Pacheco
    • , Arlet Loza-Huerta
    • , Alicia Sampieri
    • , Linda Wordeman
    • , Claudia Moreno
    •  & Luis Vaca
  • Article
    | Open Access

    Huang et al. use proteomics to examine liver and liver mitochondria of hibernating bats. The authors observe the unfolded protein response in endoplasmic reticulum and co-activation of survival signaling pathways in torpid bats and provide further insight into physiological adaptations that allow bats to survive a long period of hibernation.

    • Wenjie Huang
    • , Chen-Chung Liao
    • , Yijie Han
    • , Junyan Lv
    • , Ming Lei
    • , Yangyang Li
    • , Qingyun Lv
    • , Dong Dong
    • , Shuyi Zhang
    • , Yi-Husan Pan
    •  & Jian Luo
  • Article
    | Open Access

    By performing an ecologically relevant experiment with variety in litter composition and detritivore species, Joly et al. find that litter conversion into faeces has a profound effect on organic matter lability and decomposition. These findings have implications for biogeochemical models of carbon cycling.

    • François-Xavier Joly
    • , Sylvain Coq
    • , Mathieu Coulis
    • , Jean-François David
    • , Stephan Hättenschwiler
    • , Carsten W. Mueller
    • , Isabel Prater
    •  & Jens-Arne Subke
  • Article
    | Open Access

    By combining whole genome sequencing and metabolomics, Koshiba et al. identify a large number of associations between genetic variants and plasma metabolites. They find that some genetic variants affect the interaction between the host and symbiotic bacteria. This study provides insights into how genetic and environmental factors interact to shape human metabolism.

    • Seizo Koshiba
    • , Ikuko N. Motoike
    • , Daisuke Saigusa
    • , Jin Inoue
    • , Yuichi Aoki
    • , Shu Tadaka
    • , Matsuyuki Shirota
    • , Fumiki Katsuoka
    • , Gen Tamiya
    • , Naoko Minegishi
    • , Nobuo Fuse
    • , Kengo Kinoshita
    •  & Masayuki Yamamoto
  • Article
    | Open Access

    Stephen Doyle et al. report the chromosome-scale genome assembly and transcriptome sequence data of Haemonchus contortus, a key parasitic nematode model. These data show nearly complete conservation of chromosome content with C. elegans and brings insight into transcriptional regulation and chromosome-wide genetic diversity in this important pathogen.

    • Stephen R. Doyle
    • , Alan Tracey
    • , Roz Laing
    • , Nancy Holroyd
    • , David Bartley
    • , Wojtek Bazant
    • , Helen Beasley
    • , Robin Beech
    • , Collette Britton
    • , Karen Brooks
    • , Umer Chaudhry
    • , Kirsty Maitland
    • , Axel Martinelli
    • , Jennifer D. Noonan
    • , Michael Paulini
    • , Michael A. Quail
    • , Elizabeth Redman
    • , Faye H. Rodgers
    • , Guillaume Sallé
    • , Muhammad Zubair Shabbir
    • , Geetha Sankaranarayanan
    • , Janneke Wit
    • , Kevin L. Howe
    • , Neil Sargison
    • , Eileen Devaney
    • , Matthew Berriman
    • , John S. Gilleard
    •  & James A. Cotton
  • Article
    | Open Access

    Branconi et al. report on an experimental approach to investigate the ecological and social drivers of cooperative breeding in clownfish. Experiments were performed on a wild population in Papua New Guinea and indicate that clownfish non-breeding strategies can be understood using economic bargaining theory and cooperative breeding theory.

    • Rebecca Branconi
    • , Tina A. Barbasch
    • , Robin K. Francis
    • , Maya Srinivasan
    • , Geoffrey P. Jones
    •  & Peter M. Buston
  • Article
    | Open Access

    Maria Teschler-Nicola et al. use ancient DNA sequencing to report the earliest known case of human monozygotic twins found in a previously discovered Upper Palaeolithic burial site. Using bioanthropological and archaeological techniques, they also find that the twins were full-term newborns and that ancient mortuary behavior included re-opening of grave sites to bury related individuals together.

    • Maria Teschler-Nicola
    • , Daniel Fernandes
    • , Marc Händel
    • , Thomas Einwögerer
    • , Ulrich Simon
    • , Christine Neugebauer-Maresch
    • , Stefan Tangl
    • , Patrick Heimel
    • , Toni Dobsak
    • , Anika Retzmann
    • , Thomas Prohaska
    • , Johanna Irrgeher
    • , Douglas J. Kennett
    • , Iñigo Olalde
    • , David Reich
    •  & Ron Pinhasi
  • Article
    | Open Access

    Osakabe et al. report the implementation of a CRISPR type I-D (TiD) system for editing in plants, which consists of eight Cas genes (Cas1d–Cas7d, Cas10d) followed by an array of repeat spacer units. The CRISPR TiD can effectively induce short indels, long-range deletions and bi-allelic mutations in plants, adding a valuable toolbox to manipulate plant genomes.

    • Keishi Osakabe
    • , Naoki Wada
    • , Tomoko Miyaji
    • , Emi Murakami
    • , Kazuya Marui
    • , Risa Ueta
    • , Ryosuke Hashimoto
    • , Chihiro Abe-Hara
    • , Bihe Kong
    • , Kentaro Yano
    •  & Yuriko Osakabe