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  • Article
    | Open Access

    Workenhe et al. show in mice that a combination of oncolytic HSV-1 virus and Mitomycin-C activates an inflammatory response, through necroptosis induction, that renders tumours susceptible to immune checkpoint inhibitors. These findings informs on the potential role of necroptosis in immunotherapy approaches.

    • Samuel T. Workenhe
    • , Andrew Nguyen
    • , David Bakhshinyan
    • , Jiarun Wei
    • , David N. Hare
    • , Kelly L. MacNeill
    • , Yonghong Wan
    • , Andrew Oberst
    • , Jonathan L. Bramson
    • , Jalees A. Nasir
    • , Alyssa Vito
    • , Nader El-Sayes
    • , Sheila K. Singh
    • , Andrew G. McArthur
    •  & Karen L. Mossman
  • Article
    | Open Access

    The authors report a single cell-resolution gene expression atlas for the developing mouse liver and gallbladder using a transgenic Foxa2eGFP mouse line. By tracing the development of cells from gut endoderm to hepatoblasts they identify key transcriptional changes during liver specification.

    • Tianhao Mu
    • , Liqin Xu
    • , Yu Zhong
    • , Xinyu Liu
    • , Zhikun Zhao
    • , Chaoben Huang
    • , Xiaofeng Lan
    • , Chengchen Lufei
    • , Yi Zhou
    • , Yixun Su
    • , Luang Xu
    • , Miaomiao Jiang
    • , Hongpo Zhou
    • , Xinxin Lin
    • , Liang Wu
    • , Siqi Peng
    • , Shiping Liu
    • , Susanne Brix
    • , Michael Dean
    • , Norris R. Dunn
    • , Kenneth S. Zaret
    • , Xin-Yuan Fu
    •  & Yong Hou
  • Article
    | Open Access

    Gowripalan, Smith et al. use CRISPR/Cas9 technology to rapidly select recombinant poxviruses without using selectable marker genes. They find that Cas9 cleavage inhibits poxvirus DNA replication, suppressing virus spread in culture. This application makes poxviruses more attractive vector platforms for fighting cancer and emerging disease outbreaks.

    • Anjali Gowripalan
    • , Stewart Smith
    • , Tijana Stefanovic
    •  & David C. Tscharke
  • Article
    | Open Access

    Nirav Chhabra et al. characterize adult mice carrying a homozygous mutation in Pax6 that was identified in a patient with foveal hypoplasia. They find that the Pax6 point mutation has pleiotropic effects, including defects in the mouse retinal structures, loss of the optic nerve, changes in energy metabolism and circadian rhythms, and dysregulation of genes expressed in the pancreas.

    • Nirav Florian Chhabra
    • , Oana Veronica Amarie
    • , Moya Wu
    • , Anna-Lena Amend
    • , Marina Rubey
    • , Daniel Gradinger
    • , Martin Irmler
    • , Johannes Beckers
    • , Birgit Rathkolb
    • , Eckhard Wolf
    • , Annette Feuchtinger
    • , Peter Huypens
    • , Raffaele Teperino
    • , Jan Rozman
    • , Gerhard K. H. Przemeck
    •  & Martin Hrabě de Angelis
  • Article
    | Open Access

    Ruhlandt et al. report a plasmonic nanocavity-based method to measure absolute values of quantum yield of commonly used fluorescent proteins. The method is calibration-free, does not require knowledge about maturation or potential dark states, and works on minute amounts of sample. Authors are further able to determine lifetime and quantum yield of several fluorescent proteins, which would be a good resource for researchers working with them.

    • Daja Ruhlandt
    • , Martin Andresen
    • , Nickels Jensen
    • , Ingo Gregor
    • , Stefan Jakobs
    • , Jörg Enderlein
    •  & Alexey I. Chizhik
  • Article
    | Open Access

    Joseph Korpela et al. demonstrate the use of machine-learning assisted bio-loggers on black-tailed gulls and streaked shearwaters. As video recording is only activated through variations in movement detected by low-cost accelerometers, this method represents improvements to runtime and precision over existing bio-logging technology.

    • Joseph Korpela
    • , Hirokazu Suzuki
    • , Sakiko Matsumoto
    • , Yuichi Mizutani
    • , Masaki Samejima
    • , Takuya Maekawa
    • , Junichi Nakai
    •  & Ken Yoda
  • Article
    | Open Access

    Lee et al. describe the generation of aglycosylated antibody-producing mice. These aglycosylated antibodies, lacking glycans prevent unwanted interactions with the lectins, and are used as reagents in a tool they developed called ALIQUAT. This aglycosylated antibody and lectin-based immunoassay diagnostic platform can be used to detect disease specific glycan biomarkers.

    • Nan-Ee Lee
    • , Sun Hee Kim
    • , Dae-Yeul Yu
    • , Eui-Jeon Woo
    • , Myung-Il Kim
    • , Gi-Sang Seong
    • , Sun Min Lee
    • , Jeong-Heon Ko
    •  & Yong-Sam Kim
  • Article
    | Open Access

    Zhang et al. characterize the functions of two distinct arginine kinase genes in flour beetles. Using RNA interference and electophoretic mobility shift assays, they identify Broad-Complex transcription factor as the mediator of opposing hormonal regulation in these genes.

    • Nan Zhang
    • , Heng Jiang
    • , Xiangkun Meng
    • , Kun Qian
    • , Yaping Liu
    • , Qisheng Song
    • , David Stanley
    • , Jincai Wu
    • , Yoonseong Park
    •  & Jianjun Wang
  • Article
    | Open Access

    Shi et al. use solid-state nuclear magnetic resonance spectroscopy to reveal the internal dynamics of human histones hH3 and hH4 in the Widom 601 and the telomeric nucleosome core particles. This work has implications for the propagation of epigenetic changes via the center of the nucleosome core communicating with histone tails and DNA.

    • Xiangyan Shi
    • , Chinmayi Prasanna
    • , Aghil Soman
    • , Konstantin Pervushin
    •  & Lars Nordenskiöld
  • Article
    | Open Access

    Iain Timmins et al. identify 70 loci associated with self-reported walking pace in the UK Biobank. Using Mendelian randomization, they find evidence for a causal link between increased walking pace and lower cardiometabolic risk profile, which combined with a low estimated heritability, suggests self-reported walking pace could be a target for healthcare intervention.

    • Iain R. Timmins
    • , Francesco Zaccardi
    • , Christopher P. Nelson
    • , Paul W. Franks
    • , Thomas Yates
    •  & Frank Dudbridge
  • Article
    | Open Access

    Seo et al. identify fatty acid-binding protein 5 (FABP5) as a booster of HIF-1α activity. They find that oleic-acid treatment activates the FABP5/HIF-1α axis, promoting lipid accumulation and cell proliferation in liver cancer cells. This study provides insights into how fatty acids drive the progression of cancer.

    • Jieun Seo
    • , Do-Won Jeong
    • , Jong-Wan Park
    • , Kwang-Woong Lee
    • , Junji Fukuda
    •  & Yang-Sook Chun
  • Article
    | Open Access

    Blumental-Perry et al. find that exposure to cigarette smoke causes the redistribution of the mitochondria-encoded non-codling RNA, mito-ncR-805, from mitochondria to the nucleus in alveolar epithelial cells. This relocation event correlates with increased expression of nuclear genes involved in mitochondrial function, suggesting that this response has a protective role.

    • A. Blumental-Perry
    • , R. Jobava
    • , I. Bederman
    • , A. J. Degar
    • , H. Kenche
    • , B. J. Guan
    • , K. Pandit
    • , N. A. Perry
    • , N. D. Molyneaux
    • , J. Wu
    • , E. Prendergas
    • , Z.-W. Ye
    • , J. Zhang
    • , C. E. Nelson
    • , F. Ahangari
    • , D. Krokowski
    • , S. H. Guttentag
    • , P. A. Linden
    • , D. M. Townsend
    • , A. Miron
    • , M.-J. Kang
    • , N. Kaminski
    • , Y. Perry
    •  & M. Hatzoglou
  • Article
    | Open Access

    Scarola et al. identify a conserved OCT4 pseudogene mechanism of action and demonstrate that the OCT4 pseudogene lncRNA is required for FUS-dependent loading of the SUV39H1 histone methyltransferase to the promoter of the parental OCT4 gene.

    • Michele Scarola
    • , Elisa Comisso
    • , Massimo Rosso
    • , Giannino Del Sal
    • , Claudio Schneider
    • , Stefan Schoeftner
    •  & Roberta Benetti
  • Article
    | Open Access

    Yang, Xu, Gu et al. demonstrate that activation of endogenous Sox17 pushes fibroblasts to an extraembryonic endoderm-like state in chemically induced reprogramming of somatic cells into stem cells. This study provides insights into how chemicals prime the transition of somatic cells into stem cells.

    • Zhenghao Yang
    • , Xiaochan Xu
    • , Chan Gu
    • , Jun Li
    • , Qihong Wu
    • , Can Ye
    • , Alexander Valentin Nielsen
    • , Lichao Mao
    • , Junqing Ye
    • , Ke Bai
    • , Fan Guo
    • , Chao Tang
    •  & Yang Zhao
  • Article
    | Open Access

    Ku, Kim et al develop a method to analyse the ratio of the alternatively spliced variant of AIMP2 to full length AIMP via single-molecule RNA-FISH. They can subclassify hematologic cancer based on AIMP2-DX2/AIMP2 ratio and find that cells with high AIMP2-DX2 ratio can be sensitized to chemotherapy drugs by depleting AIMP2-DX2.

    • Jayoung Ku
    • , Ryul Kim
    • , Dongchan Kim
    • , Daeyoon Kim
    • , Seulki Song
    • , Keonyong Lee
    • , Namseok Lee
    • , MinA Kim
    • , Sung-Soo Yoon
    • , Nam Hoon Kwon
    • , Sunghoon Kim
    • , Yoosik Kim
    •  & Youngil Koh
  • Article
    | Open Access

    Casali, Tognolina et al. use two-photon laser microscopy to spatially map long-term synaptic plasticity in rat cerebellar granular cells following stimulation of mossy fibers. Their data allow them to apply realistic modeling to test hypotheses about the synaptic spiking dynamics and reveal the importance of synaptic inhibition to defining these microcircuits.

    • Stefano Casali
    • , Marialuisa Tognolina
    • , Daniela Gandolfi
    • , Jonathan Mapelli
    •  & Egidio D’Angelo
  • Article
    | Open Access

    Kimura et al. reports a refined human skin equivalent (HSE) model that reproduces traction-force balance in the lateral direction. This tension improves HSE characteristics and promotes skin homeostasis. This model has great potential for applications in drug screening and understanding the molecular mechanisms of drug effects, skin ageing and diseases.

    • Shun Kimura
    • , Ayako Tsuchiya
    • , Miho Ogawa
    • , Miki Ono
    • , Nao Suda
    • , Kaori Sekimoto
    • , Makoto Takeo
    •  & Takashi Tsuji
  • Article
    | Open Access

    Lindsay Stewart et al. analyze clinical isolates of the human malaria parasite Plasmodium falciparum from a highly endemic West African population and show that intrinsic multiplication rate variation is associated with blood-stage infection intensity. Their results indicate that parasite control of multiplication contributes to virulence.

    • Lindsay B. Stewart
    • , Ofelia Diaz-Ingelmo
    • , Antoine Claessens
    • , James Abugri
    • , Richard D. Pearson
    • , Sonia Goncalves
    • , Eleanor Drury
    • , Dominic P. Kwiatkowski
    • , Gordon A. Awandare
    •  & David J. Conway
  • Article
    | Open Access

    Amanda Melin et al. compare variation in 29 primate species at 12 amino acid residue sites coded by the ACE2 gene and show that apes and African and Asian monkeys exhibit the same set of twelve key amino acid residues as human ACE2. These results suggest that these primates are likely to be susceptible to SARS-CoV-2, whereas ACE2 gene sequences and protein-protein interaction models suggest reduced susceptibility for platyrrhines, tarsiers, lorisoids, and some lemurs.

    • Amanda D. Melin
    • , Mareike C. Janiak
    • , Frank Marrone III
    • , Paramjit S. Arora
    •  & James P. Higham
  • Article
    | Open Access

    Hechenleitner et al. describe two new titanosaurians and the finding of numerous accumulations of titanosaurian eggs in La Rioja, Argentina. This study suggests nesting site philopatry among Titanosauria and that this clade was spread throughout southern South America at the end of the Late Cretaceous.

    • E. Martín Hechenleitner
    • , Léa Leuzinger
    • , Agustín G. Martinelli
    • , Sebastián Rocher
    • , Lucas E. Fiorelli
    • , Jeremías R. A. Taborda
    •  & Leonardo Salgado
  • Article
    | Open Access

    Bleasdale et al. examine the introduction of agricultural crops in the Congo Basin with stable isotope analysis of human and animal remains, charred food remains, and plant microparticles from dental calculus. Their findings reveal variation in the adoption of cereals from the early Iron Age, and provide long-term insights into changing human reliance on different resources.

    • Madeleine Bleasdale
    • , Hans-Peter Wotzka
    • , Barbara Eichhorn
    • , Julio Mercader
    • , Amy Styring
    • , Jana Zech
    • , María Soto
    • , Jamie Inwood
    • , Siobhán Clarke
    • , Sara Marzo
    • , Bianca Fiedler
    • , Veerle Linseele
    • , Nicole Boivin
    •  & Patrick Roberts
  • Article
    | Open Access

    Honma et al. have developed a method of producing high-sugar rice grains using gene knock-out technology. This grain has the potential to advance development of alternative sugar crops in the future.

    • Yujiro Honma
    • , Prakash Babu Adhikari
    • , Keiko Kuwata
    • , Tomoko Kagenishi
    • , Ken Yokawa
    • , Michitaka Notaguchi
    • , Kenichi Kurotani
    • , Erika Toda
    • , Kanako Bessho-Uehara
    • , Xiaoyan Liu
    • , Shaowei Zhu
    • , Xiaoyan Wu
    •  & Ryushiro D. Kasahara
  • Article
    | Open Access

    Kozlovskii and Popov present BiteNet, a new computational method utilizing deep learning principles for rapid detection of binding sites. BiteNet considers proteins as 3D images, enabling rapid detection of allosteric sites from either static protein structures or its dynamic ensembles.

    • Igor Kozlovskii
    •  & Petr Popov
  • Article
    | Open Access

    Brell, Berg et al find that iron enters cells not only through iron-transferrin uptake by the transferrin receptor (CD71) but also through uptake of heme-albumin by this receptor and that heme-albumin stimulates proliferation in a manner dependent on heme oxygenase 1. This study presents a new route for iron uptake in mammalian cells.

    • Brell Jennifer
    • , Verena Berg
    • , Madhura Modak
    • , Alexander Puck
    • , Maria Seyerl-Jiresch
    • , Sarojinidevi Künig
    • , Gerhard J. Zlabinger
    • , Peter Steinberger
    • , Janet Chou
    • , Raif S. Geha
    • , Leopold Öhler
    • , Akihiro Yachie
    • , Hyeryun Choe
    • , Markus Kraller
    • , Hannes Stockinger
    •  & Johannes Stöckl
  • Article
    | Open Access

    Wu et al. discover a role for Arl8b-dependent lysosomal trafficking and exocytosis in promoting invasion and metastasis of breast cancer cells and tumours that survive irradiation treatment. These findings provide insights that may aid future strategies to improve radiotherapy outcome.

    • Ping-Hsiu Wu
    • , Yasuhito Onodera
    • , Amato J. Giaccia
    • , Quynh-Thu Le
    • , Shinichi Shimizu
    • , Hiroki Shirato
    •  & Jin-Min Nam
  • Article
    | Open Access

    Xindong Chen et al. simulate the sheet-like branched actin networks used by cells during cell migration using a novel 3D assembling model. They show how intracellular proteins regulate the network’s elastic properties and then affect cell migration, providing greater insight into the fundamental physical mechanisms of the previously published experimental observations.

    • Xindong Chen
    • , Hanxing Zhu
    • , XiQiao Feng
    • , Xiaona Li
    • , Yongtao Lu
    • , Zuobin Wang
    •  & Yacine Rezgui
  • Article
    | Open Access

    Davide Raineri, Chiara Dianzani et al. show that osteopontin binds ICOSL at a different domain than the one used by ICOS. Activation of ICOSL by osteopontin induces cell migration in vitro and tumor metastatization in a 4T1 breast cancer mouse model; highlighting the functional role of this interaction in cancer progression.

    • Davide Raineri
    • , Chiara Dianzani
    • , Giuseppe Cappellano
    • , Federica Maione
    • , Gianluca Baldanzi
    • , Ilaria Iacobucci
    • , Nausicaa Clemente
    • , Giulia Baldone
    • , Elena Boggio
    • , Casimiro L. Gigliotti
    • , Renzo Boldorini
    • , Josè M. Rojo
    • , Maria Monti
    • , Leila Birolo
    • , Umberto Dianzani
    •  & Annalisa Chiocchetti
  • Article
    | Open Access

    Millie Heo et al. report a quantitative assessment of the immunization-induced antibacterial IgG repertoire in mice at single-antibody resolution. They find that immunization with multiple strains of heat-killed bacteria results in higher frequencies of bacteria-binding IgG, but with the tradeoff of lower binding affinities.

    • Millie Heo
    • , Guilhem Chenon
    • , Carlos Castrillon
    • , Jérôme Bibette
    • , Pierre Bruhns
    • , Andrew D. Griffiths
    • , Jean Baudry
    •  & Klaus Eyer
  • Article
    | Open Access

    Wijerathne et al develop an approach to quantify mRNA amounts in extracellular vesicles (EVs) from plasma using a microfluidics device to enrich EVs. They show this method allows for the correlation of specific mRNAs with acute ischemic stroke pointing towards potential clinical use.

    • Harshani Wijerathne
    • , Malgorzata A. Witek
    • , Joshua M. Jackson
    • , Virginia Brown
    • , Mateusz L. Hupert
    • , Kristina Herrera
    • , Cameron Kramer
    • , Abigail E. Davidow
    • , Yan Li
    • , Alison E. Baird
    • , Michael C. Murphy
    •  & Steven A. Soper
  • Article
    | Open Access

    Jorge Doña, Andrew Sweet and Kevin Johnson find that dove lice species with higher dispersal abilities have stronger genomic signatures of introgression. By using sequence data from multiple species of both wing and body lice from the same species of hosts, the authors are able to control for nearly all factors besides dispersal ability, demonstrating the power of this study system.

    • Jorge Doña
    • , Andrew D. Sweet
    •  & Kevin P. Johnson
  • Article
    | Open Access

    Laruelle et al. present a fitting based computational method called SET that can detect hidden spatial structure in cell tissue organization in a statistically significant manner using a single image and without any parameter settings. They exemplify the utility of the approach by identifying invisible cell and subcellular patterns.

    • Elise Laruelle
    • , Nathalie Spassky
    •  & Auguste Genovesio
  • Article
    | Open Access

    Hou et al demonstrate that L-arginine supplementation enhances intestinal stem cell function in mice. Co-culture experiments show that this effect is due to Wnt2b section by CD90+ stromal cells. These findings show an important role for stroma in nutrient-stimulated stem cell expansion.

    • Qihang Hou
    • , Yuanyang Dong
    • , Jingxi Huang
    • , Chaoyong Liao
    • , Jiaqi Lei
    • , Youli Wang
    • , Yujiao Lai
    • , Yifei Bian
    • , Yang He
    • , Jingjing Sun
    • , Meng Sun
    • , Qiuyu Jiang
    • , Bo Wang
    • , Zhengquan Yu
    • , Yuming Guo
    •  & Bingkun Zhang
  • Article
    | Open Access

    To enhance the accuracy of CRISPR-Cas9 editing, Matsumoto et al have fused the anti-CRISPR protein AcrIIA4 with Cdt1, a protein that is degraded in S and G2, the phases of the cell cycle where homology-directed repair (HDR) dominates. This leads to an increase in HDR and in on-target editing, illustrating the feasibility of the approach.

    • Daisuke Matsumoto
    • , Hirokazu Tamamura
    •  & Wataru Nomura
  • Article
    | Open Access

    Poulet et al investigate the role for the PRL-2 phosphatase in mouse retina, and find that loss of PRL-2 impairs vascular outgrowth and arteriovenous patterning. They find that PRL-2 s regulates endothelial cell migration and sprouting angiogenesis and acts through VEGF and NOTCH1 signalling.

    • Mathilde Poulet
    • , Jacinthe Sirois
    • , Kevin Boyé
    • , Noriko Uetani
    • , Serge Hardy
    • , Thomas Daubon
    • , Alexandre Dubrac
    • , Michel L. Tremblay
    •  & Andreas Bikfalvi
  • Article
    | Open Access

    Here, the authors report a genome-wide association study for appendicular lean mass using more than 450,000 participants in the UK Biobank Study. They identify 799 loci at genome-wide significance, explaining over 15% of phenotypic variance and >25% of the heritability, allowing the authors to uncover the genetic architecture of this sarcopenia-related trait.

    • Yu-Fang Pei
    • , Yao-Zhong Liu
    • , Xiao-Lin Yang
    • , Hong Zhang
    • , Gui-Juan Feng
    • , Xin-Tong Wei
    •  & Lei Zhang
  • Article
    | Open Access

    Ren, Xiao et al. identify a mechanism by which myostatin regulates adipogenesis, using myostatin-knockout pigs. Myostatin deficiency upregulates MEF2C that binds to the promoter of miR222. miR222 in turn downregulates stearoyl-CoA desaturase 5. This study provides potential targets that can be engineered to generate a new pig variety that has high leanness while maintaining its high intramuscular fat content.

    • Hongyan Ren
    • , Wei Xiao
    • , Xingliang Qin
    • , Gangzhi Cai
    • , Hao Chen
    • , Zaidong Hua
    • , Cheng Cheng
    • , Xinglei Li
    • , Wenjun Hua
    • , Hongwei Xiao
    • , Liping Zhang
    • , Jiali Dai
    • , Xinmin Zheng
    • , Zhe Zhu
    • , Chong Qian
    • , Jie Yao
    •  & Yanzhen Bi
  • Article
    | Open Access

    Previous work has suggested potential links between Mycobacterium tuberculosis infection and the development of both lung cancer and sarcoidosis, in addition to tuberculosis. Here, Qiyao Chai, Zhe Lu, Zhidong Liu and colleagues report a transcriptomic analysis of lung tissue from tuberculosis, lung adenocarcinoma, and sarcoidosis patients and find that while many disease-linked expression changes are shared between the three diseases, each also has distinct transcriptional signatures that could be useful as molecular markers.

    • Qiyao Chai
    • , Zhe Lu
    • , Zhidong Liu
    • , Yanzhao Zhong
    • , Fuzhen Zhang
    • , Changgen Qiu
    • , Bingxi Li
    • , Jing Wang
    • , Lingqiang Zhang
    • , Yu Pang
    •  & Cui Hua Liu
  • Article
    | Open Access

    Varela, Roch et al. find that resistance of preceding isolates to carbapenems is associated with the subsequent isolation of methicillin-resistant Staphylococcus aureus (MRSA) resistant to ceftaroline. Addition of meropenem reduces the cystic fibrosis MRSA’s resistance to ceftaroline in a pneumonia mouse model, suggesting a strategy to control antibiotics resistance.

    • Maria Celeste Varela
    • , Melanie Roch
    • , Agustina Taglialegna
    • , Scott W. Long
    • , Matthew Ojeda Saavedra
    • , Warren E. Rose
    • , James J. Davis
    • , Lucas R. Hoffman
    • , Rafael E. Hernandez
    • , Roberto R. Rosato
    •  & Adriana E. Rosato
  • Article
    | Open Access

    Bahareh Taghizadeh and Nicholas Foley et al. show that individual neuronal responses, population spiking activity, and local field potential oscillations encode the variance of expected rewards independent of their value. They also demonstrate that reward uncertainty asymmetrically affects neuronal transmission within the monkey fronto-parietal network.

    • Bahareh Taghizadeh
    • , Nicholas C. Foley
    • , Saeed Karimimehr
    • , Michael Cohanpour
    • , Mulugeta Semework
    • , Sameer A. Sheth
    • , Reza Lashgari
    •  & Jacqueline Gottlieb
  • Article
    | Open Access

    Sinha et al. demonstrate that overexpression of centrosomal protein Cep55 in mice is sufficient to cause a wide-spectrum of cancer via multiple mechanisms including hyperactivation of the Pi3k/Akt pathway, stabilized microtubules and a defective replication checkpoint response. These findings are relevant to human cancers as high CEP55 expression is associated with worse prognosis across multiple cancer types.

    • Debottam Sinha
    • , Purba Nag
    • , Devathri Nanayakkara
    • , Pascal H. G. Duijf
    • , Andrew Burgess
    • , Prahlad Raninga
    • , Veronique A. J. Smits
    • , Amanda L. Bain
    • , Goutham Subramanian
    • , Meaghan Wall
    • , John. W. Finnie
    • , Murugan Kalimutho
    •  & Kum Kum Khanna
  • Article
    | Open Access

    Lobo et al. find that phosphodiesterase 2A2 (PDE2A2) regulates a local pool of cAMP that controls PKA-dependent phosphorylation of MIC60 at the mitochondrial contact site and organizing system and influences Parkin recruitment and mitophagy. They also find that PDE2A2 inhibition promotes adipocyte browning, with implications for metabolic disorders and obesity.

    • Miguel J. Lobo
    • , Laia Reverte-Salisa
    • , Ying-Chi Chao
    • , Andreas Koschinski
    • , Frank Gesellchen
    • , Gunasekaran Subramaniam
    • , He Jiang
    • , Samuel Pace
    • , Natasha Larcom
    • , Ester Paolocci
    • , Alexander Pfeifer
    • , Sara Zanivan
    •  & Manuela Zaccolo
  • Article
    | Open Access

    Junglas et al. probe into the mechanism of membrane stabilization by the inner membrane-associated protein of 30 kDa (IM30), a member of the phage shock protein A (PspA) family, and report that ring-shaped IM30 super-complexes disassemble upon binding to negatively charged membrane surfaces, involving partly unfolding of the monomers and formation of a membrane-protecting carpet. This study highlights the structural role of intrinsically disordered proteins in membrane stabilization.

    • Benedikt Junglas
    • , Roberto Orru
    • , Amelie Axt
    • , Carmen Siebenaller
    • , Wieland Steinchen
    • , Jennifer Heidrich
    • , Ute A. Hellmich
    • , Nadja Hellmann
    • , Eva Wolf
    • , Stefan A. L. Weber
    •  & Dirk Schneider
  • Article
    | Open Access

    Sebastian Bobadilla-Suarez et al. evaluate the brain-wide organization of subjective value and confidence, as determined by inverse decision entropy, using a mode-based fMRI analysis of subjects performing a mixed gambling task. They showed that these signals are strongly intertwined and are organized into a systematic gradient across the human brain.

    • Sebastian Bobadilla-Suarez
    • , Olivia Guest
    •  & Bradley C. Love
  • Article
    | Open Access

    Sato, Ito, and Yamamoto report the identification of a Fruitless target gene, teiresias (tei), required for neuronal feminization in Drosophila. They find that tei is responsible for specifying the sex-specific structure of neurites and propose that the Tei protein forms a complex with robo1, conferring the ligand specificity on the heteromeric receptor complex.

    • Kosei Sato
    • , Hiroki Ito
    •  & Daisuke Yamamoto
  • Article
    | Open Access

    Petraitis and Dudgeon report a decline in mussel and gastropod abundance and recruitment on rocky intertidal shores of the Gulf of Maine over a period of 20 years. These declines are correlated with increased water temperatures and aragonite saturation state, which affects rates of shell calcification, and suggests environmental impacts on the food webs of rocky shores.

    • Peter S. Petraitis
    •  & S. R. Dudgeon
  • Article
    | Open Access

    Watanabe et al. combine two previously developed strategies to identify E3 ubiquitin ligase substrates into a method, TR-TUBE that is subsequently used to identify substrates of the Parkin and TRIM28 ligases. They identify known substrates, validating the utility of the approach, and find that TRIM28 targets Cyclin A and TFIIB for degradation.

    • Masashi Watanabe
    • , Yasushi Saeki
    • , Hidehisa Takahashi
    • , Fumiaki Ohtake
    • , Yukiko Yoshida
    • , Yusuke Kasuga
    • , Takeshi Kondo
    • , Hiroaki Yaguchi
    • , Masanobu Suzuki
    • , Hiroki Ishida
    • , Keiji Tanaka
    •  & Shigetsugu Hatakeyama
  • Article
    | Open Access

    Yubero et al. report a close connection between energy metabolism and cell stiffness in breast cancer cells, finding that healthy cell stiffness is based on ATP-driven actin polymerization, whereas in metastatic cells it is based on ATP-driven myosin II activity. They show that noninvasive cancerous cells exhibit an anomalous behavior, as their stiffness is little affected by the lack of nutrients and energy.

    • Marina. L. Yubero
    • , Priscila M. Kosaka
    • , Álvaro San Paulo
    • , Marcos Malumbres
    • , Montserrat Calleja
    •  & Javier Tamayo
  • Article
    | Open Access

    Fernández-Quintero et al. employ molecular dynamics simulations in combination with experimental structural information to demonstrate that antibodies exist as several interconverting paratope states. They propose that dynamic conformational transitions on the micro-to-millisecond timescale are responsible for antibody allostery in contrast to the long believed paradigm of static canonical structures determining binding properties and specificity of antibodies.

    • Monica L. Fernández-Quintero
    • , Nancy D. Pomarici
    • , Barbara A. Math
    • , Katharina B. Kroell
    • , Franz Waibl
    • , Alexander Bujotzek
    • , Guy Georges
    •  & Klaus R. Liedl