Browse Articles

  • Article
    | Open Access

    Cai and colleagues sequence and annotate the genome of the yellowstripe goby at the chromosome level. Combining genome and transcriptome data, they infer key metabolic pathways for high lipid accumulation in the liver of this fish.

    • Lei Cai
    • , Guocheng Liu
    • , Yuanzheng Wei
    • , Yabing Zhu
    • , Jianjun Li
    • , Zongyu Miao
    • , Meili Chen
    • , Zhen Yue
    • , Lujun Yu
    • , Zhensheng Dong
    • , Huixin Ye
    • , Wenjing Sun
    •  & Ren Huang
  • Article
    | Open Access

    Here, the authors discover that NGF, upregulated by transcription factor ZBTB46 in prostate cancer exposed to androgen therapy, promotes neuroendocrine differentiation. They show that NGF interacts with the GPCR CHRM4, that both NGF and CHRM4 are upregulated in highly metastatic prostate cancer and that targeting NGF reduces therapy resistance in a mouse xenograft model.

    • Wei-Yu Chen
    • , Yu-Ching Wen
    • , Shian-Ren Lin
    • , Hsiu-Lien Yeh
    • , Kuo-Ching Jiang
    • , Wei-Hao Chen
    • , Yow-Sien Lin
    • , Qingfu Zhang
    • , Phui-Ly Liew
    • , Michael Hsiao
    • , Jiaoti Huang
    •  & Yen-Nien Liu
  • Article
    | Open Access

    Savva et al. investigate the sex dependent effect of maternal obesity on the metabolism of offspring. They find that metabolic changes are conferred on a sex basis, with males suffering from impaired lipid metabolism relative to females when born to obese mothers, using a lipidomic approach.

    • Christina Savva
    • , Luisa A. Helguero
    • , Marcela González-Granillo
    • , Daniela Couto
    • , Tânia Melo
    • , Xidan Li
    • , Bo Angelin
    • , Maria Rosário Domingues
    • , Claudia Kutter
    •  & Marion Korach-André
  • Article
    | Open Access

    Zhao et al. demonstrate the effect of elevated extracellular K + on hidden hearing loss in mice, and the consequent effect on inner hair cell ribbon synapse loss. Their results indicate that BK potassium channels could be a potential target for treatment of hidden hearing loss.

    • Hong-Bo Zhao
    • , Yan Zhu
    •  & Li-Man Liu
  • Article
    | Open Access

    Li, Cebrian et al. show that a combination of peptide D-11 and vancomycin exhibits a potent antimicrobial activity against a panel of Gram-negative pathogens without apparent toxicity. They find that this combination is also effective in clearing Pseudomonas aeruginosa in mice, providing a potential antimicrobial therapy for humans.

    • Qian Li
    • , Rubén Cebrián
    • , Manuel Montalbán-López
    • , Huan Ren
    • , Weihui Wu
    •  & Oscar P. Kuipers
  • Article
    | Open Access

    Dowsett et al use a single-cell resolution approach to analyse the distribution of mutations across several divisions in yeast diploid strains mutated in mismatch repair and polymerase delta proofreading. They find that the underlying mutation rate varies from one division to another, and that new mutations segregate unequally between sister chromatids at each division, expanding genetic heterogeneity in the population.

    • Ian T. Dowsett
    • , Jessica L. Sneeden
    • , Branden J. Olson
    • , Jill McKay-Fleisch
    • , Emma McAuley
    • , Scott R. Kennedy
    •  & Alan J. Herr
  • Article
    | Open Access

    Kato, Abdollahi et al. identify a redox protein thioredoxin and mitochondrial fission-1 (FIS1) protein as miR-379 targets in mouse kidney. They find that miR-379 knockout mice are protected from diabetic kidney disease by enhancing mitophagy via FIS1, suggesting miR-379 as a potential therapeutic target for diabetic kidney disease.

    • Mitsuo Kato
    • , Maryam Abdollahi
    • , Ragadeepthi Tunduguru
    • , Walter Tsark
    • , Zhuo Chen
    • , Xiwei Wu
    • , Jinhui Wang
    • , Zhen Bouman Chen
    • , Feng-Mao Lin
    • , Linda Lanting
    • , Mei Wang
    • , Janice Huss
    • , Patrick T Fueger
    • , David Chan
    •  & Rama Natarajan
  • Article
    | Open Access

    Natalia Tikhenko et al. investigate the genetic contribution of the wheat chromosome 1D to its development and evolution. They find a novel locus, DEFECTIVE ENDOSPERM-D1, on the long arm of 1D that is required for normal endosperm development as its absence leads to non-viable grains and altered grain shape.

    • Natalia Tikhenko
    • , Ahmad M. Alqudah
    • , Lioudmilla Borisjuk
    • , Stefan Ortleb
    • , Twan Rutten
    • , DanDan Wu
    • , Manuela Nagel
    • , Axel Himmelbach
    • , Martin Mascher
    • , Marion S. Röder
    • , Martin W. Ganal
    • , Stefanie Sehmisch
    • , Andreas Houben
    •  & Andreas Börner
  • Article
    | Open Access

    Sonal Prasad et al. identify several mutations in the radixin (RDX) gene that are associated with early-life hearing loss. Using a guinea pig model, they propose that radixin helps convert sound into electrical signals in the mature inner ear.

    • Sonal Prasad
    • , Barbara Vona
    • , Marta Diñeiro
    • , María Costales
    • , Rocío González-Aguado
    • , Ana Fontalba
    • , Clara Diego-Pérez
    • , Asli Subasioglu
    • , Guney Bademci
    • , Mustafa Tekin
    • , Rubén Cabanillas
    • , Juan Cadiñanos
    •  & Anders Fridberger
  • Article
    | Open Access

    Steven Chen et al. develop an automated, time-lapsed, high-content screen to quantify the responses of Schistosoma mansoni larvae to chemical insult. They apply their method to evaluate 45 static and kinetic response endpoints for seven drugs and screen 1323 approved drugs. Their work identifies anti-schistosomal compounds and underscores the value of quantifying motion in phenotypic drug discovery.

    • Steven Chen
    • , Brian M. Suzuki
    • , Jakob Dohrmann
    • , Rahul Singh
    • , Michelle R. Arkin
    •  & Conor R. Caffrey
  • Article
    | Open Access

    DeNies et al. identify a new mechanism of intracellular GPCR signalling. Using CXC chemokine receptor 4 (CXCR4) as a model, they show that upon stimulation with receptor agonists that not only plasma membrane-localized receptors, but also intracellular CXCR4 molecules are post-translationally modified and regulate transcription. This study suggests that a small pool of plasma membrane-localized GPCRs can activate internal receptor-dependent signaling, and that β-arrestin-1 mediates this activation.

    • Maxwell S. DeNies
    • , Alan V. Smrcka
    • , Santiago Schnell
    •  & Allen P. Liu
  • Article
    | Open Access

    Yao Yao et al. perform transcriptome-wide association analysis through integration of gene expression data and results of a previously-published genome-wide association study for neutrophil count. They find 34 independent genomic regions associated with expression changes and show that knockout of one candidate gene, TAF9, in CD34+ progenitor cells increases neutrophil maturation in vitro.

    • Yao Yao
    • , Jia Yang
    • , Qian Qin
    • , Chao Tang
    • , Zhidan Li
    • , Li Chen
    • , Kailong Li
    • , Chunyan Ren
    • , Lu Chen
    •  & Shuquan Rao
  • Article
    | Open Access

    Oguchi et al. developed an in-house sequencing system to spatially decode DNA barcode molecules at single-molecule resolution. They obtain an average read length of 20 nucleotides with an error rate of less than 5% per nucleotide. Leveraging this system, they devised a molecular foot printing method that can be applied spatial proteomics as well as spatial transcriptomics.

    • Yusuke Oguchi
    • , Hirofumi Shintaku
    •  & Sotaro Uemura
  • Article
    | Open Access

    Dumitru et al. probe the ligand-binding mechanism and activation of the human G protein-coupled C5a receptor (C5aR) and discover a cooperativity between the two orthosteric binding sites. Their findings, probing the dynamic aspects of receptor–ligand interaction, are valuable to develop a better understanding of GPCR activation and signalling.

    • Andra C. Dumitru
    • , R. N. V. Krishna Deepak
    • , Heng Liu
    • , Melanie Koehler
    • , Cheng Zhang
    • , Hao Fan
    •  & David Alsteens
  • Article
    | Open Access

    Wu et al. demonstrate utility of a novel, ultrahigh ductility Mg alloy-based biodegradable stent in vitro and in vivo for use in congenital disorders like pediatric laryngotracheal stenosis. Tested in both bioreactor system and rabbit airway model, these stents display higher corrosion resistance and better in vivo degradation without affecting growth of rabbit airway.

    • Jingyao Wu
    • , Leila J. Mady
    • , Abhijit Roy
    • , Ali Mübin Aral
    • , Boeun Lee
    • , Feng Zheng
    • , Toma Catalin
    • , Youngjae Chun
    • , William R. Wagner
    • , Ke Yang
    • , Humberto E. Trejo Bittar
    • , David Chi
    •  & Prashant N. Kumta
  • Article
    | Open Access

    Qianxing Mo et al. identify basal and luminal integrative subtypes of muscle-invasive bladder cancer (MIBC) using multi-omics data from The Cancer Genome Atlas. Using a gene panel for classification of MIBC derived from gene expression data, they find that the basal subtype is associated with worse survival in patients receiving no neoadjuvant chemotherapy (NAC), but better survival in patients receiving cisplatin-based NAC and further identify genes associated with response to PD-L1 blockade immunotherapy, suggesting potential clinical use of these genes’ expression signature.

    • Qianxing Mo
    • , Roger Li
    • , Dennis O. Adeegbe
    • , Guang Peng
    •  & Keith Syson Chan
  • Article
    | Open Access

    Tapia-Alveal, Olsen and Worgall develop a novel strategy for patient-specific multiple myeloma diagnostics platform using DNA aptamers. The high affinity DNA aptamers enabled detection of minimal residual disease (MRD) when conventional MRD methods assessed complete remission and are adaptable to multiple diagnostic formats including point-of-care devices.

    • Claudia Tapia-Alveal
    • , Timothy R. Olsen
    •  & Tilla S. Worgall
  • Article
    | Open Access

    Nina Muñoz et al. present a novel thermal ablation technique, termed molecularly targeted photothermal ablation (MTPA) that generates hyperthermia specifically within tumor tissue in a clinically-relevant rat model of liver cancer. They further show that MTPA modulates the intratumoral immune profile toward stronger tumor immunogenicity while reducing the release of oncogenic cytokines relative to conventional ablation techniques.

    • Nina M. Muñoz
    • , Crystal Dupuis
    • , Malea Williams
    • , Katherine Dixon
    • , Amanda McWatters
    • , Rony Avritscher
    • , Richard Bouchard
    • , Ahmed Kaseb
    • , Kyle M. Schachtschneider
    • , Arvind Rao
    •  & Rahul A. Sheth
  • Article
    | Open Access

    Kennedy et al. report the pharmacological and in vivo profiling of two small molecule PAR2 inhibitors and an agonist. They conclude that while the small molecule agonist and one of the inhibitors bind to the orthosteric PAR2 binding site, the other inhibitor is a negative allosteric modulator, highlighting two distinct mechanisms of inhibition that could be targeted for future development of drugs that modulate PAR2.

    • Amanda J. Kennedy
    • , Linda Sundström
    • , Stefan Geschwindner
    • , Eunice K. Y. Poon
    • , Yuhong Jiang
    • , Rongfeng Chen
    • , Rob Cooke
    • , Shawn Johnstone
    • , Andrew Madin
    • , Junxian Lim
    • , Qingqi Liu
    • , Rink-Jan Lohman
    • , Anneli Nordqvist
    • , Maria Fridén-Saxin
    • , Wenzhen Yang
    • , Dean G. Brown
    • , David P. Fairlie
    •  & Niek Dekker
  • Article
    | Open Access

    Jinghua Yang et al. provide the de novo assemblies of the transcriptomes of wasabi, horseradish and mustard to examine the evolution of specialized glucosinolates. This study demonstrates convergent evolution of super-accumulation of sinigrin in these taxa and may allow for potential strategies to engineer metabolic pathways of bioactive compounds for human food sources or pharmaceutical purposes.

    • Jinghua Yang
    • , Zhangping Li
    • , Jinmin Lian
    • , Guoning Qi
    • , Pibiao Shi
    • , Jiawei He
    • , Zhongyuan Hu
    •  & Mingfang Zhang
  • Article
    | Open Access

    Yu et al. provide evidence that Ustilago maydis employs two structurally distinct telomere-binding proteins to perform complementary functions in telomere maintenance in this organism. While UmTay1 regulates primarily telomere replication and recombination, UmTrf2 is responsible for telomere protection.

    • Eun Young Yu
    • , Syed S. Zahid
    • , Swapna Ganduri
    • , Jeanette H. Sutherland
    • , Min Hsu
    • , William K. Holloman
    •  & Neal F. Lue
  • Article
    | Open Access

    Romel Somwar et al. find that cancer-causing NTRK gene fusions resistant to one form of inhibitor therapy can be resistant to other inhibitor types. Using molecular simulations, they show that some NTRK1 mutations resistant to the type I inhibitor larotrectinib are hypersensitive to the type II inhibitor altiratinib, potentially due to the introduction of a sulfur moiety in the kinase binding pocket.

    • Romel Somwar
    • , Nicolle E. Hofmann
    • , Bryan Smith
    • , Igor Odintsov
    • , Morana Vojnic
    • , Irina Linkov
    • , Ashley Tam
    • , Inna Khodos
    • , Marissa S. Mattar
    • , Elisa de Stanchina
    • , Daniel Flynn
    • , Marc Ladanyi
    • , Alexander Drilon
    • , Ujwal Shinde
    •  & Monika A. Davare
  • Article
    | Open Access

    Using single-cell RNA sequencing, Dong, Miao, Wang et al. profile the transcriptomes of 21,292 cells from biopsies of 6 castration-resistant prostate cancers. They find that all neuroendocrine tumor cells display a luminal-like epithelial phenotype, providing insights into the cellular states underlying neuroendocrine transdifferentiation in human prostate cancer.

    • Baijun Dong
    • , Juju Miao
    • , Yanqing Wang
    • , Wenqin Luo
    • , Zhongzhong Ji
    • , Huadong Lai
    • , Man Zhang
    • , Xiaomu Cheng
    • , Jinming Wang
    • , Yuxiang Fang
    • , Helen He Zhu
    • , Chee Wai Chua
    • , Liancheng Fan
    • , Yinjie Zhu
    • , Jiahua Pan
    • , Jia Wang
    • , Wei Xue
    •  & Wei-Qiang Gao
  • Article
    | Open Access

    Takayoshi Ishii et al. report that two functional centromere-specific histone H3 (CENH3) paralogs with different centromere occupancy among different tissue types present in diploid cowpea. They demonstrate that CENH3.1 of cowpea is essential for normal plant growth and reproduction, while CENH3.2 is a gene likely to be undergoing early pseudogenization.

    • Takayoshi Ishii
    • , Martina Juranić
    • , Shamoni Maheshwari
    • , Fernanda de Oliveira Bustamante
    • , Maximilian Vogt
    • , Rigel Salinas-Gamboa
    • , Steven Dreissig
    • , Nial Gursanscky
    • , Tracy How
    • , Dmitri Demidov
    • , Joerg Fuchs
    • , Veit Schubert
    • , Andrew Spriggs
    • , Jean-Philippe Vielle-Calzada
    • , Luca Comai
    • , Anna M. G. Koltunow
    •  & Andreas Houben
  • Article
    | Open Access

    Yuan et al. characterise metabolic profiles of human mesenchymal stem cells (hMSCs) during cell expansion in culture. They find that late passage hMSCs exhibit a NAD + /NADH redox cycle imbalance and that adding NAD + precursor nicotinamide restores mitochondrial fitness and cellular homeostasis in senescent hMSCs indicating a possible route to preserve hMSC homeostasis for therapeutic use.

    • Xuegang Yuan
    • , Yijun Liu
    • , Brent M. Bijonowski
    • , Ang-Chen Tsai
    • , Qin Fu
    • , Timothy M. Logan
    • , Teng Ma
    •  & Yan Li
  • Review Article
    | Open Access

    Laflamme and Mekhail discuss emerging nuclear roles for LLPS in genome organization, gene expression and DNA repair, highlighting the emerging notion that biomolecular condensates regulate the sequential engagement of molecules in multistep biological processes.

    • Guillaume Laflamme
    •  & Karim Mekhail
  • Article
    | Open Access

    Ramesh Bondada et al. report natural epimutations in the Arabidopsis SUPERMAN gene from tetraploid and diploid accessions. The existence of these epialleles in diverse wild populations have the potential to shed light on the evolution of unisexuality in plants.

    • Ramesh Bondada
    • , Saravanakumar Somasundaram
    • , Mohan Premanand Marimuthu
    • , Mohammed Afsal Badarudeen
    • , Vaishak Kanjirakol Puthiyaveedu
    •  & Ravi Maruthachalam
  • Editorial
    | Open Access

    Centrosomes are the main microtubule-organizing centers in animal cells, indispensable for cell division and the building of a wide range of cilia, which include sensory and motile cilia. We are now inviting submissions related to the fascinating field of centrosomes, cilia and all of the processes that they are involved in with the aim of highlighting this work in a Special Collection.

    • Tiago J. Dantas
  • Article
    | Open Access

    Narayanan et al. show that local administration of miR-210 accelerates wound healing specifically in diabetic mice. They find that miR-210 restores the metabolic balance in diabetic wounds by activating glycolysis and cellular migration. This study presents miR-210 as a potential therapeutic option to treat diabetic wound healing.

    • Sampath Narayanan
    • , Sofie Eliasson Angelstig
    • , Cheng Xu
    • , Jacob Grünler
    • , Allan Zhao
    • , Wan Zhu
    • , Ning Xu Landén
    • , Mona Ståhle
    • , Jingping Zhang
    • , Mircea Ivan
    • , Raluca Georgiana Maltesen
    • , Ileana Ruxandra Botusan
    • , Neda Rajamand Ekberg
    • , Xiaowei Zheng
    •  & Sergiu-Bogdan Catrina
  • Article
    | Open Access

    Xuejing Wen et al. present a new method, Reverse Chromatin Immunoprecipitation (R-ChIP), for analyzing DNA-protein interactions specifically in plant cells. They apply R-ChIP to identify proteins binding to the promoter of the Arabidopsis gene AtCAT3 and optimize the method using transient transformation of the target promoter to increase efficiency.

    • Xuejing Wen
    • , Jingxin Wang
    • , Daoyuan Zhang
    • , Yu Ding
    • , Xiaoyu Ji
    • , Zilong Tan
    •  & Yucheng Wang
  • Article
    | Open Access

    Simon Bachler et al. present a new microfluidic platform to control the precise position and patterns of nanoliter droplets with various lipid materials. They show their platform enables monitoring of droplets and subsequent label-free mass spectrometry, which represents an important advance for the synthetic biology community.

    • Simon Bachler
    • , Dominik Haidas
    • , Marion Ort
    • , Todd A. Duncombe
    •  & Petra S. Dittrich
  • Article
    | Open Access

    With cryo-EM, single-molecule FRET and MD simulations, Iadanza et al. characterise the membrane protein insertase complex BAM in lipid bilayer nanodiscs. They show that the β-barrel domain of BamA is in a ‘lateral open’ conformation, and that BAM-containing lipid nanodisc deform around BAM’s lateral gate, giving structural evidence for lipid ‘disruptase’ activity of BAM.

    • Matthew G. Iadanza
    • , Bob Schiffrin
    • , Paul White
    • , Matthew A. Watson
    • , Jim E. Horne
    • , Anna J. Higgins
    • , Antonio N. Calabrese
    • , David J. Brockwell
    • , Roman Tuma
    • , Antreas C. Kalli
    • , Sheena E. Radford
    •  & Neil A. Ranson
  • Article
    | Open Access

    Eric Jorgenson and Hélène Choquet et al. find that northwestern European genetic ancestry is associated with increased risk of cutaneous squamous cell carcinoma (cSCC) in non-Hispanic whites, and more so in Hispanic/Latinos of the US. The ancestry effect is largely, but not entirely explained by genetic determinants of skin pigmentation in both populations.

    • Eric Jorgenson
    • , Hélène Choquet
    • , Jie Yin
    • , Thomas J. Hoffmann
    • , Yambazi Banda
    • , Mark N. Kvale
    • , Neil Risch
    • , Catherine Schaefer
    •  & Maryam M. Asgari
  • Article
    | Open Access

    Lili Ren et al. investigate antibody response from 176 COVID-19 patients who presented different stages of their disease. This study suggests the late onset of antibody response as a risk factor for disease severity and a limited role of antibody titers in predicting the disease severity of COVID-19.

    • Lili Ren
    • , Lulu Zhang
    • , De Chang
    • , Junwen Wang
    • , Yongfeng Hu
    • , Hong Chen
    • , Li Guo
    • , Chao Wu
    • , Conghui Wang
    • , Yingying Wang
    • , Ying Wang
    • , Geng Wang
    • , Siyuan Yang
    • , Charles S. Dela Cruz
    • , Lokesh Sharma
    • , Linghang Wang
    • , Dingyu Zhang
    •  & Jianwei Wang
  • Article
    | Open Access

    Peterle et al. show that a subtilisin like serine protease secreted from gut microbiota Bacillus subtilis cleaves the wild-type human transthyretin (hTTR) to generate an amyloidogenic peptide. High propensity of the hTTR fragment to form pathogenic protein aggregates implicates the serine protease in the pathogenesis of acquired senile systemic amyloidosis.

    • Daniele Peterle
    • , Giulia Pontarollo
    • , Stefano Spada
    • , Paola Brun
    • , Luana Palazzi
    • , Alexej V. Sokolov
    • , Barbara Spolaore
    • , Patrizia Polverino de Laureto
    • , Vadim B. Vasilyev
    • , Ignazio Castagliuolo
    •  & Vincenzo De Filippis
  • Article
    | Open Access

    Aluthge et al. evaluated the establishment of human fecal bacterial communities in human microbiota-associated (HMA) piglet and mouse models under identical dietary conditions. They find that human fecal bacterial communities from mature microbiotas establish more successfully in the HMA porcine model than mice, suggesting the broader applicability of the piglet model for human microbiome studies.

    • Nirosh D. Aluthge
    • , Wesley A. Tom
    • , Alison C. Bartenslager
    • , Thomas E. Burkey
    • , Phillip S. Miller
    • , Kelly D. Heath
    • , Craig Kreikemeier-Bower
    • , Hatem Kittana
    • , Robert J. Schmaltz
    • , Amanda E. Ramer-Tait
    •  & Samodha C. Fernando
  • Article
    | Open Access

    Ella Gabitov et al. use functional magnetic resonance imaging to identify changes in neural activity when participants commit an error in a motor sequence task. Their results suggest that two distinct neural circuits are involved in the processing of action errors: (1) the salience network that signals surprise and novelty, and (2) the reward pathway that plays a central role in motivation and incentive drive.

    • Ella Gabitov
    • , Ovidiu Lungu
    • , Geneviève Albouy
    •  & Julien Doyon
  • Article
    | Open Access

    Matiar Jafari, Tyson Aflalo et al. show that the human primary somatosensory cortex is activated when subjects imagine reaches in a cognitive motor task, but not when they plan movement or imagine a static limb position. These results highlight a role for this region in cognitive imagery and motor control in the absence of sensory information.

    • Matiar Jafari
    • , Tyson Aflalo
    • , Srinivas Chivukula
    • , Spencer Sterling Kellis
    • , Michelle Armenta Salas
    • , Sumner Lee Norman
    • , Kelsie Pejsa
    • , Charles Yu Liu
    •  & Richard Alan Andersen
  • Article
    | Open Access

    Han Zhang et al. report molecular dynamics simulations and mutational analysis of a key process of the E. coli glycine cleavage system, an important enzyme complex in C1 metabolism. They identify a key amino acid residue controlling the release of the swinging aminomethyl lipoate arm and increase the overall reaction rate of glycine cleavage by more than twice, providing a strategy for manipulating this reaction system for use in synthetic biology.

    • Han Zhang
    • , Yuchen Li
    • , Jinglei Nie
    • , Jie Ren
    •  & An-Ping Zeng
  • Article
    | Open Access

    Here the authors report a major histocompatibility complex (MHC) association analysis for nasopharyngeal carcinoma in Chinese individuals from Hong Kong, finding 8 independent associated loci associated with lower risk for developing nasopharyngeal carcinoma. Two non-human leukocyte antigen (HLA) genes are E3 ubiquitin ligases, TRIM31 and TRIM39, having a role in the innate immune response and implicating the importance of host Epstein-Barr virus interactions in this cancer.

    • Lvwen Ning
    • , Josephine Mun-Yee Ko
    • , Valen Zhuoyou Yu
    • , Hoi Yan Ng
    • , Candy King-Chi Chan
    • , Lihua Tao
    • , Shiu-Yeung Lam
    • , Merrin Man-Long Leong
    • , Roger Kai-Cheong Ngan
    • , Dora Lai-Wan Kwong
    • , Anne Wing-Mui Lee
    • , Wai-Tong Ng
    • , Ashley Cheng
    • , Stewart Tung
    • , Victor Ho-Fun Lee
    • , Ka-On Lam
    • , Chung-Kong Kwan
    • , Wing-Sum Li
    • , Stephen Yau
    • , Jin-Xin Bei
    •  & Maria Li Lung
  • Article
    | Open Access

    Here, the authors screen for feedback regulation of ribosomal proteins by overexpressing a non- regulatable version of a gene and observing its effects on the expression of the GFP-fused version. They find that 30% show at least a 3-fold reduction in expression and two genes show a 10-fold reduction with the regulatory site being in the 5’ untranslated region of the gene.

    • Basab Roy
    • , David Granas
    • , Fredrick Bragg Jr.
    • , Jonathan A. Y. Cher
    • , Michael A. White
    •  & Gary D. Stormo
  • Article
    | Open Access

    Here, the authors report a multi-ethnic genome wide association meta-analysis of 12 studies from the International Cataract Genetics Consortium. They find six new loci associated with age-related nuclear cataract, in addition to replicating the association at CRYAA, and suggest a strong genetic link between age-related nuclear and congenital cataracts.

    • Ekaterina Yonova-Doing
    • , Wanting Zhao
    • , Robert P. Igo Jr
    • , Chaolong Wang
    • , Periasamy Sundaresan
    • , Kristine E. Lee
    • , Gyungah R. Jun
    • , Alexessander Couto Alves
    • , Xiaoran Chai
    • , Anita S. Y. Chan
    • , Mei Chin Lee
    • , Allan Fong
    • , Ava G. Tan
    • , Chiea Chuen Khor
    • , Emily Y. Chew
    • , Pirro G. Hysi
    • , Qiao Fan
    • , Jacqueline Chua
    • , Jaeyoon Chung
    • , Jiemin Liao
    • , Johanna M. Colijn
    • , Kathryn P. Burdon
    • , Lars G. Fritsche
    • , Maria K. Swift
    • , Maryam H. Hilmy
    • , Miao Ling Chee
    • , Milly Tedja
    • , Pieter W. M. Bonnemaijer
    • , Preeti Gupta
    • , Queenie S. Tan
    • , Zheng Li
    • , Eranga N. Vithana
    • , Ravilla D. Ravindran
    • , Soon-Phaik Chee
    • , Yuan Shi
    • , Wenting Liu
    • , Xinyi Su
    • , Xueling Sim
    • , Yang Shen
    • , Ya Xing Wang
    • , Hengtong Li
    • , Yih-Chung Tham
    • , Yik Ying Teo
    • , Tin Aung
    • , Kerrin S. Small
    • , Paul Mitchell
    • , Jost B. Jonas
    • , Tien Yin Wong
    • , Astrid E. Fletcher
    • , Caroline C. W. Klaver
    • , Barbara E. K. Klein
    • , Jie Jin Wang
    • , Sudha K. Iyengar
    • , Christopher J. Hammond
    •  & Ching-Yu Cheng
  • Article
    | Open Access

    Here, the authors report a genome-wide association analysis for corneal resistance factor (CRF), a measure of corneal biomechanical properties relevant to a number of corneal diseases. They identify 135 loci associated with CRF and use fine-mapping and ATAC-seq in human cornea cell lines to identify putative causal variants affecting gene expression.

    • Xinyi Jiang
    • , Nefeli Dellepiane
    • , Erola Pairo-Castineira
    • , Thibaud Boutin
    • , Yatendra Kumar
    • , Wendy A. Bickmore
    •  & Veronique Vitart
  • Article
    | Open Access

    Reyes et al. identified and characterized dominant mutations in the MutL homolog MLH2 (in S. cerevisiae) and PMS1 (in humans) causing an increased mutator phenotype. Based on their findings, they propose that Mlh2/hPMS1-Mlh1 mutant complexes can act as roadblocks on DNA preventing mismatch repair function.

    • Gloria X. Reyes
    • , Boyu Zhao
    • , Tobias T. Schmidt
    • , Kerstin Gries
    • , Matthias Kloor
    •  & Hans Hombauer
  • Article
    | Open Access

    Ye, Xiong et al. present crystal structures of bacterial E3 ubiquitin ligase IpaH9.8 and IpaH9.8LRR–hGBP1. They find that substrate-binding destabilizes the hydrophobic cluster to relieve the autoinhibition of IpaH9.8. This study provides insights into the mechanisms underlying substrate-induced activation of IpaH9.8.

    • Yuxin Ye
    • , Yuxian Xiong
    •  & Hao Huang
  • Article
    | Open Access

    In this work, the authors demonstrate that a coral thermostable GFP (TGP) is an improved tag compared to conventional GFPs. In addition to reporting melting point stability at temperatures near 90 °C, its fusion also helps increase expression levels of test membrane proteins. They further generated synthetic nanobodies against TGP to facilitate purification.

    • Hongmin Cai
    • , Hebang Yao
    • , Tingting Li
    • , Cedric A. J. Hutter
    • , Yanfang Li
    • , Yannan Tang
    • , Markus A. Seeger
    •  & Dianfan Li
  • Article
    | Open Access

    Ren et al. investigated how the obligate intracellular pathogen Toxoplasma gondii satisfies its requirement of phosphatidylinositol (PtdIns). They show that this parasite can synthesize certain PtdIns species de novo and salvages others from human host cells. Endogenous production of PtdIns in the Golgi complex is critical for the acute infection, and its loss perturbs homeostasis of selected anionic phospholipids.

    • Bingjian Ren
    • , Pengfei Kong
    • , Fatima Hedar
    • , Jos F. Brouwers
    •  & Nishith Gupta
  • Article
    | Open Access

    de Medeiros and Farrell test how insect-host interactions influence species divergence in phytophagous insects. Using ddRAD-seq data and hierarchical modelling, they demonstrate, surprisingly, that antagonistic interactions between insect and host do not lead to greater species diversification.

    • Bruno A. S. de Medeiros
    •  & Brian D. Farrell