Browse Articles

  • Article
    | Open Access

    From a fermenting bacterium, Thermotoga maritima, Kuhns, Trifunovi ć et al. purify a complex that includes a respiratory enzyme, Rnf. They find that the Rnf complex requires Na+ for activity and that it catalyzes Na+ transport in liposomes. This study shows that the Rnf complex is indeed an ion translocating, respiratory enzyme.

    • Martin Kuhns
    • , Dragan Trifunović
    • , Harald Huber
    •  & Volker Müller
  • Article
    | Open Access

    Radford et al. monitored cattle marked with artificial eyespots in non-commercial cattle farms in northern Botswana, and demonstrate that cattle with these eyespot markings were more likely to avoid predation by large carnivores. Their findings have applications as a cost-effective tool to reduce livestock predation by ambush predators.

    • Cameron Radford
    • , John Weldon McNutt
    • , Tracey Rogers
    • , Ben Maslen
    •  & Neil Jordan
  • Article
    | Open Access

    Ravasio et al. develop an assay to quantify the clustering of Eph receptor EphA2 upon ligand binding, based on the scoring of single cells. They discover that clustering phenotype predicts cell and population migration potential in cancer cells and reflects Eph associated gene expression profiles in cancer cell lines.

    • Andrea Ravasio
    • , Myint Z. Myaing
    • , Shumei Chia
    • , Aditya Arora
    • , Aneesh Sathe
    • , Elaine Yiqun Cao
    • , Cristina Bertocchi
    • , Ankur Sharma
    • , Bakya Arasi
    • , Vin Yee Chung
    • , Adrianne C. Green
    • , Tuan Zea Tan
    • , Zhongwen Chen
    • , Hui Ting Ong
    • , N. Gopalakrishna Iyer
    • , Ruby YunJu Huang
    • , Ramanuj DasGupta
    • , Jay T. Groves
    •  & Virgile Viasnoff
  • Article
    | Open Access

    Shinzawa et al. develop a method that improves the transfection efficiency for Plasmodium parasites that cause malaria by transfecting Cas9-expressing parasites with linear donor templates. This method allows efficient integration of the donor template while preventing unexpected recombination.

    • Naoaki Shinzawa
    • , Tsubasa Nishi
    • , Fumiya Hiyoshi
    • , Daisuke Motooka
    • , Masao Yuda
    •  & Shiroh Iwanaga
  • Article
    | Open Access

    Mochizuki, Fukumoto, Ohara, et al. study the protective role of the rare sugar d-tagatose against plant disease. They focus on its effects against the downy mildews and delineate the metabolic pathway that blocks the initial infection. Their work paves the way for the development of safe fungicidal agrochemicals using natural products.

    • Susumu Mochizuki
    • , Takeshi Fukumoto
    • , Toshiaki Ohara
    • , Kouhei Ohtani
    • , Akihide Yoshihara
    • , Yoshio Shigematsu
    • , Keiji Tanaka
    • , Koichi Ebihara
    • , Shigeyuki Tajima
    • , Kenji Gomi
    • , Kazuya Ichimura
    • , Ken Izumori
    •  & Kazuya Akimitsu
  • Article
    | Open Access

    Hwang et al. show that the activity of PRMT5 methyltransferase is regulated by Src kinase-mediated phosphorylation at Y324 in response to DNA damage. They also show that PRMT5 participates in NHEJ repair by regulating 53BP1 protein levels and is critical for cellular survival after DNA damage.

    • Jee Won Hwang
    • , Su-Nam Kim
    • , Nayeon Myung
    • , Doona Song
    • , Gyoonhee Han
    • , Gyu-Un Bae
    • , Mark T. Bedford
    •  & Yong Kee Kim
  • Article
    | Open Access

    Ausar et al. show that the crystal structure of gdPT, a mutant pertussis toxin (PTx), is nearly identical to that of PTx. They also find that gdPT exhibits broader immunogenicity than the pertussis toxoid antigen. This study suggests a promising potential of gdPT as an improved acellular pertussis vaccine candidate due to its reduced toxicity and greater immunogenicity.

    • Salvador F. Ausar
    • , Shaolong Zhu
    • , Jessica Duprez
    • , Michael Cohen
    • , Thomas Bertrand
    • , Valérie Steier
    • , Derek J. Wilson
    • , Stephen Li
    • , Anthony Sheung
    • , Roger H. Brookes
    • , Artur Pedyczak
    • , Alexey Rak
    •  & D. Andrew James
  • Article
    | Open Access

    Maxwell Scott et al. report the genome of the New World screwworm, a devastating livestock pest in South America and the Caribbean. Using transcriptomic analysis of the insect’s developmental stages combined with genomic analyses, the authors identify genes that may be important for its parasitic lifestyle.

    • Maxwell J. Scott
    • , Joshua B. Benoit
    • , Rebecca J. Davis
    • , Samuel T. Bailey
    • , Virag Varga
    • , Ellen O. Martinson
    • , Paul V. Hickner
    • , Zainulabeuddin Syed
    • , Gisele A. Cardoso
    • , Tatiana T. Torres
    • , Matthew T. Weirauch
    • , Elizabeth H. Scholl
    • , Adam M. Phillippy
    • , Agustin Sagel
    • , Mario Vasquez
    • , Gladys Quintero
    •  & Steven R. Skoda
  • Article
    | Open Access

    Wang et al. describe a strategy for quantitatively performing composition analysis of heparan sulfate using 13C-labeled disaccharides and polysaccharides that mimic the glycosaminoglycan chemical structure. They further show that these isotopically labelled small molecules can be used as internal standards for mass spectrometry analysis of heparan sulfate derived from mouse tissue and plasma.

    • Zhangjie Wang
    • , Katelyn Arnold
    • , Yongmei Xu
    • , Vijayakanth Pagadala
    • , Guowei Su
    • , Hannah Myatt
    • , Robert J. Linhardt
    •  & Jian Liu
  • Article
    | Open Access

    Yandong Zhang, et al. develop iDentification and qUantification sEparaTion (DUET) to quantify target protein and its post-translational modification isoforms in single cells. Using their method, they report heterogeneities in the ubiquitination levels of histone H2B in single yeast cells and reveal the cell-cycle dynamics of H2B monoubiquitination.

    • Yandong Zhang
    • , Changho Sohn
    • , Seoyoen Lee
    • , Heejeong Ahn
    • , Jinyoung Seo
    • , Junyue Cao
    •  & Long Cai
  • Article
    | Open Access

    Zhang, Wu, Feng et al. show that ligand-bound glutamine binding protein assumes multiple metastable binding sites, presenting a more dynamic energy landscape than its ligand-free form. This study provides insights into the ligand-binding mechanisms coupled with protein dynamics that underly the apparent binding affinity.

    • Lu Zhang
    • , Shaowen Wu
    • , Yitao Feng
    • , Dan Wang
    • , Xilin Jia
    • , Zhijun Liu
    • , Jianwei Liu
    •  & Wenning Wang
  • Article
    | Open Access

    Tao Li et al. report crystal structure of MucA-MucB complex and identify a cavity region of MucB that binds PEG molecule. They further show that this hydrophobic cavity of MucB is a primary site for sensing lipid molecules and that lipid A stimulates MucB to release MucA for AlgW cleavage, helping to understand the regulation mechanism of alginate biosynthesis.

    • Tao Li
    • , Lihui He
    • , Changcheng Li
    • , Mei Kang
    • , Yingjie Song
    • , Yibo Zhu
    • , Yalin Shen
    • , Ninglin Zhao
    • , Chang Zhao
    • , Jing Yang
    • , Qin Huang
    • , Xingyu Mou
    • , Aiping Tong
    • , Jinliang Yang
    • , Zhenling Wang
    • , Chengjie Ji
    • , Hong Li
    • , Hong Tang
    •  & Rui Bao
  • Article
    | Open Access

    Vinod Kumar Singh, Arnav Rastogi, et al. study etiology of mutational signature 8 (SBS8) in human cancers. They develop genome-wide composite epigenomic context maps related to mutagenesis and DNA repair. They report that SBS8 preferentially occurs in heterochromatin and likely arises from late replication errors during cancer progression.

    • Vinod Kumar Singh
    • , Arnav Rastogi
    • , Xiaoju Hu
    • , Yaqun Wang
    •  & Subhajyoti De
  • Article
    | Open Access

    Douglas Arneson et al. develop MethylResolver that enables the deconvolution of cellular fractions from bulk DNA methylation data. Using this method, the authors resolve tumor purity-scaled immune cell-type fractions without requiring cancer-specific signatures and identify cellular fractions as prognostic factors of multiple human cancers.

    • Douglas Arneson
    • , Xia Yang
    •  & Kai Wang
  • Article
    | Open Access

    Hao, Qiao, Gao et al. show that over ten thousand oligonucleotides encoding 445 KB of digital data can be stored in cultured bacterial cells. Data storage in living cells increases the information storage capacity while enabling its economical propagation.

    • Min Hao
    • , Hongyan Qiao
    • , Yanmin Gao
    • , Zhaoguan Wang
    • , Xin Qiao
    • , Xin Chen
    •  & Hao Qi
  • Article
    | Open Access

    Yoshikatsu Hosoda et al. study the genetic basis for central corneal thickness (CCT) that is associated with keratoconus. They identify two susceptibility loci, STON2 rs2371597 and SMAD3 rs12913547, using two-step genome-wide association study (GWAS) and predictive analysis with IBM’s Watson question answering computer system, respectively.

    • Yoshikatsu Hosoda
    • , Masahiro Miyake
    • , Akira Meguro
    • , Yasuharu Tabara
    • , Sachiko Iwai
    • , Naoko Ueda-Arakawa
    • , Eri Nakano
    • , Yuki Mori
    • , Munemitsu Yoshikawa
    • , Hideo Nakanishi
    • , Chiea-Chuen Khor
    • , Seang-Mei Saw
    • , Ryo Yamada
    • , Fumihiko Matsuda
    • , Ching-Yu Cheng
    • , Nobuhisa Mizuki
    • , Akitaka Tsujikawa
    • , Kenji Yamashiro
    • , Yasuharu Tabara
    • , Takahisa Kawaguchi
    • , Kazuya Setoh
    • , Fumihiko Matsuda
    • , Yoshimitsu Takahashi
    • , Takeo Nakayama
    •  & Shinji Kosugi
  • Article
    | Open Access

    Keikhosravi et al. utilises convolutional neural network (CNN) on standard H&E stained histology images to extract information about collagen fiber arrangement and alignment. Collagen images synthesized from CNN are very similar to true collagen maps produced via second harmonic generation (SHG) and other approaches.

    • Adib Keikhosravi
    • , Bin Li
    • , Yuming Liu
    • , Matthew W. Conklin
    • , Agnes G. Loeffler
    •  & Kevin W. Eliceiri
  • Article
    | Open Access

    Using species range data, phylogenies, and fossil records of thousands of extinct and extant terrestrial vertebrates, He et al. quantify temporal changes in beta dissimilarities among zoogeographical regions surrounding the Tibetan Plateau during the Cenozoic era. This study shows how geological events underpin the evolutionary history of zoogeographical regions.

    • Jiekun He
    • , Siliang Lin
    • , Jiatang Li
    • , Jiehua Yu
    •  & Haisheng Jiang
  • Article
    | Open Access

    Priscilla Y. L. Tng et al. report a Cas13b-dependent way to suppress the transmissibility of RNA viruses chikungunya and dengue in mosquitoes. They use a virus-derived reporter and a CHIKV split replication system and demonstrate that Cas13b and gRNAs can inhibit virus-derived reporter RNAs in insect cells in vitro.

    • Priscilla Ying Lei Tng
    • , Leonela Carabajal Paladino
    • , Sebald Alexander Nkosana Verkuijl
    • , Jessica Purcell
    • , Andres Merits
    • , Philip Thomas Leftwich
    • , Rennos Fragkoudis
    • , Rob Noad
    •  & Luke Alphey
  • Article
    | Open Access

    The Na-pump accessory sub-unit phospholemman (PLM) is palmitoylated by the acyltransferase zDHHC5. Plain & Howie et al identify a binding site on zDHHC5 for the Na-pump, discover that the interaction is regulated by post-translational modifications near this binding site, and find that disrupting the zDHHC5-Na-pump interaction reduces PLM palmitoylation.

    • Fiona Plain
    • , Jacqueline Howie
    • , Jennifer Kennedy
    • , Elaine Brown
    • , Michael J. Shattock
    • , Niall J. Fraser
    •  & William Fuller
  • Article
    | Open Access

    Sun et al. analyse E. coli strains from pigs and chickens in Chinese farms and slaughterhouses, and reveal that while the tet(X4) gene is not ubiquitous across China, it is highly endemic in northwestern China. Genomic analysis of these strains shows recent and independent acquisitions of plasmids in across regions.

    • Chengtao Sun
    • , Mingquan Cui
    • , Shan Zhang
    • , Dejun Liu
    • , Bo Fu
    • , Zekun Li
    • , Rina Bai
    • , Yaxin Wang
    • , Hejia Wang
    • , Li Song
    • , Chunping Zhang
    • , Qi Zhao
    • , Jianzhong Shen
    • , Shixin Xu
    • , Congming Wu
    •  & Yang Wang
  • Article
    | Open Access

    Clabbers et al. utilize MicroED to present the structure of both apo and inhibitor-bound human carbonic anhydrase II at a high resolution to clearly identify the interaction of the inhibitor, acetazolamide. This method eases the difficulty of both crystallizing the protein and soaking the inhibitor in a smaller protein crystal.

    • Max T. B. Clabbers
    • , S. Zoë Fisher
    • , Mathieu Coinçon
    • , Xiaodong Zou
    •  & Hongyi Xu
  • Article
    | Open Access

    Hu et al. combine cDNA normalization before library preparation with a software tool to increase the capture of RNA isoform species in single-molecule RNA sequencing. They demonstrate that this approach can detect previously unknown transcripts in gastric signet-ring cell carcinomas that are not present in non-malignant tissue.

    • Yueming Hu
    • , Xing-Sheng Shu
    • , Jiaxian Yu
    • , Ming-an Sun
    • , Zewei Chen
    • , Xianming Liu
    • , Qiongfang Fang
    • , Wei Zhang
    • , Xinjie Hui
    • , Ying Ying
    • , Li Fu
    • , Desheng Lu
    • , Rakesh Kumar
    •  & Yejun Wang
  • Article
    | Open Access

    Jingyu Dai et al. study the role of tree allometry in maintaining a high carbon sequestration. They measure allometry in semi-arid forests using terrestrial laser scanning. They report that tree height as well as canopy area decline to varying degrees among individuals. They provide insights on how trees balance water consumption and carbon assimilation which enhances the survival rate of the forest trees.

    • Jingyu Dai
    • , Hongyan Liu
    • , Yongcai Wang
    • , Qinghua Guo
    • , Tianyu Hu
    • , Timothy Quine
    • , Sophie Green
    • , Henrik Hartmann
    • , Chongyang Xu
    • , Xu Liu
    •  & Zihan Jiang
  • Article
    | Open Access

    Lorena Pizarro, Meirav Leibman-Markus et al. explore the genetic mechanisms for plant innate immunity. They functionally characterize a gain of function mutation in SlNRC4a in tomato. They characterize the structure of the mutant protein and functionally demonstrate that it confers broad-spectrum resistance without triggering a hypersensitive response or negatively impacting plant growth and crop yield.

    • Lorena Pizarro
    • , Meirav Leibman-Markus
    • , Rupali Gupta
    • , Neta Kovetz
    • , Ilana Shtein
    • , Einat Bar
    • , Rachel Davidovich-Rikanati
    • , Raz Zarivach
    • , Efraim Lewinsohn
    • , Adi Avni
    •  & Maya Bar
  • Article
    | Open Access

    Tomoya Ohnuki et al. show that individual neurons in the rat perirhinal cortex coherently encoded choice directions across cue and reward epochs. Individual neurons also displayed dynamically changing contributions to population encodings between these epochs, suggesting that they flexibly coordinate to support computations associated with different epochs while holding temporally coherent representations.

    • Tomoya Ohnuki
    • , Yuma Osako
    • , Hiroyuki Manabe
    • , Yoshio Sakurai
    •  & Junya Hirokawa
  • Article
    | Open Access

    Chen, Alhassen et al. show that schizophrenia-like behavioral deficits induced by excessive prenatal methionine administration are due to in-uterus aberrations rather than through early life mother-infant interaction in mice. This study identifies the brain metabolites and transcriptomic signatures, which potentially serve as early biomarkers for schizophrenia-like behaviors.

    • Siwei Chen
    • , Wedad Alhassen
    • , Ryan Yoshimura
    • , Angele De Silva
    • , Geoffrey W. Abbott
    • , Pierre Baldi
    •  & Amal Alachkar
  • Article
    | Open Access

    Ken-ichi Kurotani, Takanori Wakatake et al. study tissue adhesion in plants. They found that the model Orobanchaceae parasitic plant Phtheirospermum japonicum can be grafted on to interfamily species. Using transcriptome profiling, the identify genes involved in tissue adhesion between distant species and demonstrate that β-1,4-glucanase plays an important role in plant parasitism.

    • Ken-ichi Kurotani
    • , Takanori Wakatake
    • , Yasunori Ichihashi
    • , Koji Okayasu
    • , Yu Sawai
    • , Satoshi Ogawa
    • , Songkui Cui
    • , Takamasa Suzuki
    • , Ken Shirasu
    •  & Michitaka Notaguchi
  • Perspective
    | Open Access

    In this Perspective, Christopher Gisriel et al. discuss the challenges in accurate assignment of co-factors in cryo-EM, particularly for chlorophylls. They explore the factors that lead to misassignment and offer suggestions for improving reliability of cryo-EM-based assignments.

    • Christopher J. Gisriel
    • , Jimin Wang
    • , Gary W. Brudvig
    •  & Donald A. Bryant
  • Article
    | Open Access

    Through X-ray crystallography and NMR, Hosszu et al. show that V127 mutation in the human prion protein induces structural changes, leading to conformational restrictions in key regions of the protein associated with prion disease. These may prevent prion formation and explain this mutation′s profound effect on prion disease.

    • Laszlo L. P. Hosszu
    • , Rebecca Conners
    • , Daljit Sangar
    • , Mark Batchelor
    • , Elizabeth B. Sawyer
    • , Stuart Fisher
    • , Matthew J. Cliff
    • , Andrea M. Hounslow
    • , Katherine McAuley
    • , R. Leo Brady
    • , Graham S. Jackson
    • , Jan Bieschke
    • , Jonathan P. Waltho
    •  & John Collinge
  • Article
    | Open Access

    Verma et al. describe a new mechanism for diethylcarbamazine action, a drug classically used to treat lymphatic filariasis and loiasis. They propose that it induces rapid temporary spastic paralysis of parasites by opening TRP channels, including TRP-2 in muscle of Brugia malayi. Inactivation of TRP channels explain its short-lasting therapeutic activity.

    • Saurabh Verma
    • , Sudhanva S. Kashyap
    • , Alan P. Robertson
    •  & Richard J. Martin
  • Article
    | Open Access

    Bailleul et al. employ histology, histochemistry and Energy Dispersive Spectroscopy to confirm the presence of disputed ovarian follicles in a specimen of fossil Cretaceous bird. These findings have implications for the evolution of the avian breeding system seen in birds today.

    • Alida M. Bailleul
    • , Jingmai O’Connor
    • , Zhiheng Li
    • , Qian Wu
    • , Tao Zhao
    • , Mario A. Martinez Monleon
    • , Min Wang
    •  & Xiaoting Zheng
  • Article
    | Open Access

    Laurent Jacob et al. develop a workflow and analytical pipeline to remove technical variation from the MINDACT microarray dataset. Their method preserved biological signals and the normalized datasets can be repurposed for the discovery of other biomarkers and signatures for breast cancer.

    • Laurent Jacob
    • , Anke Witteveen
    • , Inès Beumer
    • , Leonie Delahaye
    • , Diederik Wehkamp
    • , Jeroen van den Akker
    • , Mireille Snel
    • , Bob Chan
    • , Arno Floore
    • , Niels Bakx
    • , Guido Brink
    • , Coralie Poncet
    • , Jan Bogaerts
    • , Mauro Delorenzi
    • , Martine Piccart
    • , Emiel Rutgers
    • , Fatima Cardoso
    • , Terence Speed
    • , Laura van ’t Veer
    •  & Annuska Glas
  • Article
    | Open Access

    Yueqing Li, Xiaotong Shan, et al. study the MYB-bHLH-WD40 (MBW) regulatory network in a non-grass monocot plant, Freesia hybrida. They report two anthocyanin related MYB repressors FhMYB27 and FhMYBx and verified their involvement in a functional feedback loop with MBW to regulate anthocyanin biosynthesis.

    • Yueqing Li
    • , Xiaotong Shan
    • , Ruifang Gao
    • , Taotao Han
    • , Jia Zhang
    • , Yanan Wang
    • , Shadrack Kimani
    • , Li Wang
    •  & Xiang Gao
  • Author Correction
    | Open Access

    • Anne-Lise Peille
    • , Vincent Vuaroqueaux
    • , Swee-Seong Wong
    • , Jason Ting
    • , Kerstin Klingner
    • , Bruno Zeitouni
    • , Manuel Landesfeind
    • , Woo Ho Kim
    • , Hyuk-Joon Lee
    • , Seong-Ho Kong
    • , Isabella Wulur
    • , Steven Bray
    • , Peter Bronsert
    • , Nina Zanella
    • , Greg Donoho
    • , Han-Kwang Yang
    • , Heinz-Herbert Fiebig
    • , Christoph Reinhard
    •  & Amit Aggarwal
  • Article
    | Open Access

    With the aim to advance the development of a P. falciparum transmission blocking vaccine, Singh et al. determine the crystal structure of Pfs230D1 in complex with the Fab fragment of TB mAb 4F12. They further study the cellular localization of Pfs230 on the surface of sexual stages of parasites and the effect of combining TB mAbs against Pfs230 and Pfs48/45.

    • Kavita Singh
    • , Martin Burkhardt
    • , Sofia Nakuchima
    • , Raul Herrera
    • , Olga Muratova
    • , Apostolos G. Gittis
    • , Emily Kelnhofer
    • , Karine Reiter
    • , Margery Smelkinson
    • , Daniel Veltri
    • , Bruce J. Swihart
    • , Richard Shimp Jr.
    • , Vu Nguyen
    • , Baoshan Zhang
    • , Nicholas J. MacDonald
    • , Patrick E. Duffy
    • , David N. Garboczi
    •  & David L. Narum
  • Article
    | Open Access

    Toriyama et al. delete the glycolytic enzyme Pgam1 in T cells to investigate the role of glycolysis in T cell-mediated immune responses. They find that glycolysis, mTORC1 and glutamine affect each other and cooperate to induce T cell proliferation and differentiation.

    • Koji Toriyama
    • , Makoto Kuwahara
    • , Hiroshi Kondoh
    • , Takumi Mikawa
    • , Nobuaki Takemori
    • , Amane Konishi
    • , Toshihiro Yorozuya
    • , Takeshi Yamada
    • , Tomoyoshi Soga
    • , Atsushi Shiraishi
    •  & Masakatsu Yamashita
  • Article
    | Open Access

    By RNA sequencing of prostate cancer samples, Takayama et al identify a cluster of lncRNAs that are enriched in castration-resistant prostate cancer. This cluster is regulated by androgen receptor (AR) and associate with factors involved in AR splicing, suggesting a role for these lncRNAs in AR signalling and tumorigenesis.

    • Ken-ichi Takayama
    • , Tetsuya Fujimura
    • , Yutaka Suzuki
    •  & Satoshi Inoue
  • Article
    | Open Access

    Gonzales-Rivera et al. develop a method for 8-oxoG profiling using immunocapturing and RNA sequencing. They show that the FDFT1 transcript is susceptible to air pollution-induced oxidation, after identifying 42 transcripts that are differentially oxidized in bronchial epithelial BEAS-2B cells under air pollution conditions relative to clean air. FDFT1 oxidation affects cholesterol synthesis pathway, leading to phenotypes associated with several lung diseases.

    • Juan C. Gonzalez-Rivera
    • , Kevin C. Baldridge
    • , Dongyu S. Wang
    • , Kanan Patel
    • , Jamie C. L. Chuvalo-Abraham
    • , Lea Hildebrandt Ruiz
    •  & Lydia M. Contreras
  • Perspective
    | Open Access

    In this Perspective article, Claudio Quilodrán et al. discuss how hybridization should be considered with respect to conservation. They argue hybridization may be useful for species conservation by enhancing the ability of taxa to adapt better to changing environmental conditions or increasing genetic diversity.

    • Claudio S. Quilodrán
    • , Juan I. Montoya-Burgos
    •  & Mathias Currat
  • Article
    | Open Access

    Padhi et al. employ nanofibers with controlled structure and alignment as an extra-cellular matrix model, on which they study the exertion of forces from adherent fibroblasts. Identifying force exerting 3D perpendicular lateral protrusions, authors describe a mechanism which leads to the contraction of parallel, neighbouring fibers, and the forces needed to move and align the neighbouring fibers. These findings have relevance in understanding cancer-associated desmoplastic expansion.

    • Abinash Padhi
    • , Karanpreet Singh
    • , Janusz Franco-Barraza
    • , Daniel J. Marston
    • , Edna Cukierman
    • , Klaus M. Hahn
    • , Rakesh K. Kapania
    •  & Amrinder S. Nain
  • Article
    | Open Access

    Goldsmith et al. employ ribosome profiling to dissect how deletion of the essential autophagy component Atg12 impacts mRNA translation. They detect changes in the translation of mRNAs involved in DNA repair, centrosome clustering and cell cycle control and specifically focus on how loss of autophagy causes reduced production of BRCA2 and increased DNA damage.

    • Juliet Goldsmith
    • , Timothy Marsh
    • , Saurabh Asthana
    • , Andrew M. Leidal
    • , Deepthisri Suresh
    • , Adam Olshen
    •  & Jayanta Debnath
  • Article
    | Open Access

    Allen and Pennington et al. show that the cardiolipin-binding peptide elamipretide mitigates disease-induced fragmentation of cristae networks following cardiac ischemia reperfusion in rats. This study suggests that elamipretide targets mitochondrial membranes to sustain cristae networks, improving their bioenergetic function.

    • Mitchell E. Allen
    • , Edward Ross Pennington
    • , Justin B. Perry
    • , Sahil Dadoo
    • , Marina Makrecka-Kuka
    • , Maija Dambrova
    • , Fatiha Moukdar
    • , Hetal D. Patel
    • , Xianlin Han
    • , Grahame K. Kidd
    • , Emily K. Benson
    • , Tristan B. Raisch
    • , Steven Poelzing
    • , David A. Brown
    •  & Saame Raza Shaikh
  • Article
    | Open Access

    Xie et al. report a new computational method PRIME-3D2D to predict binding sites of protein–RNA interaction by considering protein 3D structure and RNA 2D structure. It is freely available, performs better than other binding sites predictor and is as good as PRIME to model protein–RNA complex.

    • Juan Xie
    • , Jinfang Zheng
    • , Xu Hong
    • , Xiaoxue Tong
    •  & Shiyong Liu
  • Article
    | Open Access

    Sara Lado et al. report a fine-scale analysis of genetic diversity in dromedary species across Asia and Africa. Using a genome-wide approach, the authors are able to infer patterns of migration following domestication, which follow known historic caravan routes, and identify major population expansions and bottlenecks through history.

    • Sara Lado
    • , Jean Pierre Elbers
    • , Angela Doskocil
    • , Davide Scaglione
    • , Emiliano Trucchi
    • , Mohammad Hossein Banabazi
    • , Faisal Almathen
    • , Naruya Saitou
    • , Elena Ciani
    •  & Pamela Anna Burger
  • Article
    | Open Access

    By combining advanced age and laser photocoagulation, Zhu et al. establish a novel CNV model of anti-VEGF resistance. They show that lipid accumulation in old macrophages promote angiogenesis, and that apoA-I binding protein (AIBP) inhibits angiogenesis by reducing this lipid accumulation. They further show that the combination of AIBP/apoA-I and anti-VEGF treatment overcomes anti-VEGF resistance and effectively suppresses CNV.

    • Lingping Zhu
    • , Mackenzie Parker
    • , Nduka Enemchukwu
    • , Megan Shen
    • , Guogang Zhang
    • , Qing Yan
    • , James T. Handa
    • , Longhou Fang
    •  & Yingbin Fu
  • Article
    | Open Access

    Yongfeng Liu, Xianjin Xu, Junyuan Gao et al. perform in silico screening targeting compounds that resemble both KCNQ1 and PIP2 structures and identify CP1 as a compound that could restore KCNQ1 currents after PIP2 depletion. Like PIP2, this compound can mediate VSD-pore coupling of the channel, albeit via a different mechanism from PIP2.

    • Yongfeng Liu
    • , Xianjin Xu
    • , Junyuan Gao
    • , Moawiah M. Naffaa
    • , Hongwu Liang
    • , Jingyi Shi
    • , Hong Zhan Wang
    • , Nien-Du Yang
    • , Panpan Hou
    • , Wenshan Zhao
    • , Kelli McFarland White
    • , Wenjuan Kong
    • , Alex Dou
    • , Amy Cui
    • , Guohui Zhang
    • , Ira S. Cohen
    • , Xiaoqin Zou
    •  & Jianmin Cui
  • Article
    | Open Access

    Guo, Jia et al. show that an FDA-approved Na + /K + - ATPase inhibitor ouabain reduces the burden of Zika virus infection in adult mice while protecting fetal mice from Zika virus infection-induced microcephaly. This study suggests ouabain’s therapeutic potential for Zika virus.

    • Jiao Guo
    • , Xiaoying Jia
    • , Yang Liu
    • , Shaobo Wang
    • , Junyuan Cao
    • , Bo Zhang
    • , Gengfu Xiao
    •  & Wei Wang
  • Perspective
    | Open Access

    Sannigrahi et al. discuss conformational switching that allows proteins to alter their conformation, aiding in pathogenesis by enabling cellular entry of viruses and bacterial toxins. Focussing on fusion domain of the Spike glycoprotein of SARS COV2 virus, they identify similar “switching sequences” present in diverse pathogen derived proteins.

    • Achinta Sannigrahi
    • , Nayan De
    •  & Krishnananda Chattopadhyay