Expanding the CRISPR Toolbox

The CRISPR-Cas9 system is best known for its ability to knock out or replace specific genes, via targeted cleavage of the genome. But scientists are developing many more applications, typically by using an inactive Cas9 to target other enzymes to specific genomic sites.  From transcriptional regulation to base editing, these developments are extending the range of biological questions that can be probed with CRISPR/Cas9.

View the animation on CRISPR gene editing and beyond.

View the poster on expanding the CRISPR toolbox.

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Starting with the established function of CRISPR-Cas9 to knock out or replace specific genes, this poster explores further applications of this system. It depicts transcriptional regulation with nuclease-inactive Cas9 coupled to transcription factors or to a transcriptional repressor, shows how epigenome editing is achieved with inactive Cas9 fused to histone modifying enzymes, and details how one can introduce specific base changes via fusion to a deaminase.

View the Expanding the CRISPR tool box poster.