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Method of the Year 2015

Our choice for Method of the Year 2015 is single-particle cryo-electron microscopy. A collection of articles discusses how recent technical advances, especially the development of direct-detection cameras, have enabled this structural biology technique to make impressive leaps in achievable resolution and, in turn, provide new insights about protein function. We also highlight methods to watch in the upcoming years.


The end of 'blob-ology': single-particle cryo-electron microscopy (cryo-EM) is now being used to solve macromolecular structures at high resolution.

Editorial | | Nature Methods

Recent advances in cryo-electron microscopy are enabling researchers to solve protein structures at near-atomic resolutions, expanding the biological applicability of this technique. Michael Eisenstein reports.

News Feature | | Nature Methods

Cryo-EM has emerged rapidly as a method for determining high-resolution structures of biological macromolecules. The author of this Commentary discusses just how much better this technology may get and how fast such developments are likely to happen.

Commentary | | Nature Methods

Optogenetic manipulation of neurons at cellular resolution holds promise for the dissection of neural microcircuitry.

Method to Watch | | Nature Methods

New computational tools learn complex motifs from large sequence data sets.

Method to Watch | | Nature Methods

Methods to systematically map the distribution of proteins in cells are evolving.

Method to Watch | | Nature Methods