The use of a planar sheet of light for illumination in fluorescence microscopy allows researchers to image sample volumes faster than possible with other current methods, while limiting light dosage. This collection of articles provides a brief overview of this exciting methodology and the biological research applications that it makes possible.
Top of pageEditorial
Editorial
Sheet of light
Daniel Evanko
PDF
Top of pageLight-sheet Microscopy collection
Perspective
The third dimension bridges the gap between cell culture and live tissue
Francesco Pampaloni, Emmanuel G. Reynaud & Ernst H. K. Stelzer
Nature Reviews Molecular Cell Biology 8, 839—845 (2007)
doi:10.1038/nrm2236
Abstract | Full Text | PDF
Review
Optical sectioning microscopy with planar or structured illumination
Jerome Mertz
Nature Methods 8, 811—819 (2011)
doi:10.1038/nmeth.1709
Abstract | Full Text | PDF
Correspondence
Software for bead-based registration of selective plane illumination microscopy data
Stephan Preibisch, Stephan Saalfeld, Johannes Schindelin & Pavel Tomancak
Nature Methods 7, 418—419 (2010)
doi:10.1038/nmeth0610-418
Full Text | PDF
News and Views
Omnidirectional microscopy
Michael Weber & Jan Huisken
Nature Methods 9, 656—657 (2012)
doi:10.1038/nmeth.2022
Abstract | Full Text | PDF
Research Articles
Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain
Hans-Ulrich Dodt, Ulrich Leischner, Anja Schierloh, Nina Jährling, Christoph Peter Mauch, Katrin Deininger, Jan Michael Deussing, Matthias Eder, Walter Zieglgänsberger & Klaus Becker
Nature Methods 4, 331—336 (2007)
doi:10.1038/nmeth1036
Abstract | Full Text | PDF
Deep and fast live imaging with two-photon scanned light-sheet microscopy
Thai V Truong, Willy Supatto, David S Koos, John M Choi & Scott E Fraser
Nature Methods 8, 757—762 (2011)
doi:10.1038/nmeth.1652
Abstract | Full Text | PDF
Whole-brain functional imaging at cellular resolution using light-sheet microscopy
Misha B Ahrens, Michael B Orger, Drew N Robson, Jennifer M Li & Philipp J Keller
Nature Methods 10, 413—420 (2013)
doi:10.1038/nmeth.2434
Abstract | Full Text | PDF
High-speed panoramic light-sheet microscopy reveals global endodermal cell dynamics
Benjamin Schmid, Gopi Shah, Nico Scherf, Michael Weber, Konstantin Thierbach, Citlali Pérez Campos, Ingo Roeder, Pia Aanstad & Jan Huisken
Nature Communications 4, Article number: 2207 (2013)
doi:10.1038/ncomms3207
Abstract | Full Text | PDF
Live-cell 3D super-resolution imaging in thick biological samples
Francesca Cella Zanacchi, Zeno Lavagnino, Michela Perrone Donnorso, Alessio Del Bue, Laura Furia, Mario Faretta & Alberto Diaspro
Nature Methods 9, 1047—1049 (2011)
doi:10.1038/nmeth.1744
Abstract | Full Text | PDF
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