The ability to prod stem cells into three-dimensional tissue models makes for a powerful way to study human biology. But these exciting tools are still works in progress.
Method of the Year 2017
We've chosen organoids as Method of the Year 2017, for their immense potential as tools to study human biology in health and disease. A News Feature gives a flavor of the exciting applications of these 3D constructs, and a Primer provides a short overview for newcomers to the field. Two expert Commentaries consider these tools from different angles: how cutting-edge imaging is synergizing with organoid culture to deepen our view into many biological systems, and how pluripotent stem cell-derived constructs can be used to model the human brain. As in the past, we've also chosen eight 'methods to watch' for the future.
A brief overview of stem cell-derived organoids: how they are made and what the challenges are.
It will soon be commonplace to localize gene expression in tissues.
Fluorescence nanoscopy is extending its reach into structural biology.
Sequencing and imaging bring unique aspects to genome architecture.
Increases in throughput push electrophysiology into a new era.
Recent advances in mass spectrometry imaging enable label-free molecular mapping in single cells and in 3D.
Sophisticated barcoding approaches are transforming cell lineaging.
New X-ray free-electron (XFEL) facilities will broaden access to this technology, facilitate methods development, and push boundaries in structural biology.
In the era of big data, neuroscience can profit from deep-learning approaches.