Communications Biology is inviting submissions on the topic of live microscopy – from new tools to emerging techniques, from conventional to advanced light microscopy - with the aim of publishing high-quality research devoted to advance our understanding of biology.
Reviews and Commentary
Application in health and disease
Live-cell imaging of glucose-induced metabolic coupling of β and α cell metabolism in health and type 2 diabetes
Using phasor-FLIM (Fluorescence Lifetime Imaging Microscopy), Wang et al. reveal differential usages of oxidative phosphorylation and glycolysis in living islet cells following glucose stimulation. This study suggests the applicability of FLIM as a drug discovery tool as it reports perturbations in glucose metabolism in living islet cells.
Hahn et al. present a method to visualize the endocrine human pancreas in 3D and calculate volumetric data. Using immunolabeling to visualize targets of interest and in reconstructing large tissue parts from imaged cm3-sized tissue blocks, they use their method to reveal previously unknown morphological differences in the endocrine pancreas affected with type 2 diabetes.
Spatially visualized single-cell pathology of highly multiplexed protein profiles in health and disease
Allam, Hu, et al. present SpatialViz, a suite of algorithms to explore spatial relationships in multiplexed tissue images by visualizing and quantifying single-cell granularity and anatomical complexity in diverse multiplexed tissue imaging data. The authors employ SpatialViz on 20-plex protein data in tissue sections from normal and chronic tonsillitis cases and observe GrB and CD86 coexpression and CD3 + CD4+ enrichment in diseased tonsils compared to healthy tonsils, and demonstrate the utility of SpatialViz as a wide-application spatial visualization method.
Intravital mesoscopic fluorescence molecular tomography allows non-invasive in vivo monitoring and quantification of breast cancer growth dynamics
Mehmet S. Ozturk et al. present an intravital mesoscopic fluorescence tomography (IFT) technique that is capable of tracking fluorescently labeled tumor cells inside living mice over several months. It demonstrates a promising new method for studying tumor growth dynamics in a quantitative and longitudinal fashion in-vivo.
Zhang, Redington & Gong develop FRET-based ratiometric calcium sensors with pairs of green and red fluorescent proteins. Their best performers, Twitch-GR and Twitch-NR inherit the superior photophysical properties of their constituent fluorescent proteins, enabling them to outperform existing ratiometric calcium sensors in brightness, photobleaching, and signal fidelity metrics.
A multiplexed machine-vision-based tracking system enables the high-content analysis of C. elegans behavior in several 96-well plates at a time and shows its utility for comparisons across multiple genotypes and upon drug treatment, tackling the bottlenecks in behavioural quantification.
A deep learning approach, termed wrinkle force microscopy, allows for conducting traction force microscopy by observing bright-field cell images but without using its conventional requirements such as fluorescent microbeads and confocal microscopy.
Phasor-based hyperspectral snapshot microscopy allows fast imaging of live, three-dimensional tissues for biomedical applications
Hedde et al. demonstrate the use of ultrafast phasor-based hyperspectral snapshot microscopy for biomedical imaging. This technique can improve imaging speed by 10-100 fold and enables 3D hyperspectral imaging of live tissues without using expensive and specialized hyperspectral cameras.