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The decreasing susceptibility of disease-causing microorganisms to commonly used drugs, termed Antimicrobial Resistance (AMR), is one of the most significant public health concerns facing the world today. Actively monitoring the emergence and spread of drug resistance in pathogen populations, characterising underlying molecular mechanisms and developing novel interventional strategies, are all critically important for combating the serious threats posed by AMR. This Collection aims to bring together original research in these key areas and welcomes submissions exploring AMR and countermeasures in bacterial, fungal, viral and protozoan pathogens.