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This year marks 15 years since the launch of The ISME Journal. During this time, The ISME Journal has grown into a internationally respected and leading title in microbial ecology, publishing major advances and contributing to the global understanding of microorganisms and their communities. In this collection, we present a set of content to commemorate this anniversary. Free to view for the next four weeks, you will find our top cited article each year since the journal launched, and our top downloaded content from the last two years, showcasing the leading research that The ISME Journal has published since its launch, and continues to publish today. We would like to take this chance to thank our authors, reviewers, and readers for their support and contributions to The ISME Journal, and we hope you enjoy reading this collection.