Milestones in applications of DNA sequencing
The milestones listed below correspond to key developments in the applications of DNA sequencing in new ways or to new areas. These are large topics, and we apologize for any omissions.
1977: Genome sequencing
1982: Shotgun sequencing
- The SAGE method captures 3′ tags from mRNAs, therefore introducing the idea of using a DNA sequencer to count molecules, an idea that has exploded with the later introduction of RNA-seq, chromatin immunoprecipitation followed by sequencing (ChIP–seq) and so on.
2004: Metagenome assembly
- These papers described the first integrated systems for next-generation DNA sequencing.
2010: De novo assembly of a large genome from short reads (giant panda)
2016: Human genome de novo assembly with PacBio: De novo assembly and phasing of a Korean human genome