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Epigenetics research over the last 20 years has led to an extraordinary range of discoveries, which are continuing to grow in number and scope. Fields of research span biochemistry, molecular biology, genomics, epigenomics, transcriptomics, medicine, therapeutics, our understanding of reproduction and ageing, developmental biology and evolutionary biology. This Collection presents data within epigenetics research including, but not limited to, data generated through techniques such as ChIP-seq, ATAC-seq, whole-genome DNA methylation such as WGBS or RRBS, RNA sequencing, single-cell RNA-seq (scRNA-seq), single-cell epigenetics/epigenomics, spatial transcriptomics/epigenomics, and the role of non-coding RNAs in epigenetic modulation.