Fluorescent protein biosensors

Genetically encoded (protein-based) fluorescent biosensors have been developed to enable imaging and monitoring of a variety of metabolites and cellular events, as highlighted in this collection of recent papers from Nature Portfolio. Engineering these biosensors is often non-trivial, requiring careful mutagenesis and insertion of active domains, but insights gained from past studies can be used to inform more rational design, and thus more rapid access to useful variants, in the future.

Fluorescent protein engineering:

Calcium biosensors

Metabolite biosensors

Neuronal biosensors