Focus on Europe: Advances in Translational Pathology

Content

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  Cas9-based enrichment and single-molecule sequencing for precise characterization of genomic duplications

  • Christopher M. Watson
  • Laura A. Crinnion
  • David T. Bonthron

  Technical Report4 Jul 2019 Laboratory Investigation
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  Co-staining of microRNAs and their target proteins by miRNA in situ hybridization and immunohistofluorescence on prostate cancer tissue microarrays

  The authors examined the feasibility of co-staining miRNAs by fluorescent miRNA in situ hybridization (miRisH) and their target proteins by immunohistofluorescence (IHF) on tissue microarrays from prostate cancer patients, which would allow for the study of miRNA expression patterns and their target proteins at the single-cell level. The miRNAs and corresponding target proteins include the pairs miR-145/ERG, miR-143/uPAR and miR-375/SEC23A.

  • Markus Eckstein
  • Verena Sailer
  • On behalf of the German Prostate Cancer Consortium (DPKK)

  Technical Report11 Jun 2019 Laboratory Investigation
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  Multimodal analysis of formalin-fixed and paraffin-embedded tissue by MALDI imaging and fluorescence in situ hybridization for combined genetic and metabolic analysis

  A new technical pipeline describes a combined approach of HER2/CEP17 fluorescence in situ hybridization (FISH) analysis with MALDI imaging on the very same section of formalin-fixed and paraffin-embedded (FFPE) tissue. Combining molecules detected by MALDI imaging with the gene copy number detected by HER2/CEP17 FISH, we found a synergistic effect which enhances patient prognosis.

  • Katharina Huber
  • Thomas Kunzke
  • Axel Walch

  Technical Report31 May 2019 Laboratory Investigation
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  The role of metabolic enzymes in mesenchymal tumors and tumor syndromes: genetics, pathology, and molecular mechanisms

  • Inga-Marie Schaefer
  • Jason L. Hornick
  • Judith V. M. G. Bovée

  Mini Review16 Jan 2018 Laboratory Investigation
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  Integrated tumor identification and automated scoring minimizes pathologist involvement and provides new insights to key biomarkers in breast cancer

  • Peter Bankhead
  • José A Fernández
  • Peter W Hamilton

  Pathobiology in Focus18 Dec 2017 Laboratory Investigation
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  Deep learning assisted mitotic counting for breast cancer

  Mitotic density is part of breast cancer grading yet is hampered by interobserver variability. The authors developed a deep learning algorithm which automatically locates the mitotic hotspot in breast tumors. Counting mitoses within this predefined hotspot considerably improved interobserver agreement. Mitotic counts assessed by the algorithm were comparable to the observers’ results.

  • Maschenka C. A. Balkenhol
  • David Tellez
  • Jeroen A. W. M. van der Laak

  Article20 Jun 2019 Laboratory Investigation
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  High-content, cell-by-cell assessment of HER2 overexpression and amplification: a tool for intratumoral heterogeneity detection in breast cancer

  The authors developed a high-content, quantitative analysis of breast cancer tissues based on microfluidic staining and image processing, to characterize both HER2 overexpression and amplification at the cellular level. This study paves the way for evaluatation of intratumoral heterogeneity with unprecedented accuracy with standard staining methods such as immunofluorescence and FISH.

  • Huu Tuan Nguyen
  • Daniel Migliozzi
  • Martin A. M. Gijs

  Technical Report18 Jan 2019 Laboratory Investigation
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  Novel cell enrichment technique for robust genetic analysis of archival classical Hodgkin lymphoma tissues

  This paper describes the enrichment of rare neoplastic Hodgkin and Reed-Sternberg (HRSC) cells of classical Hodgkin lymphoma (cHL) from the archival formalin-fixed and paraffin-embedded tissues. This technique allows enrichment of HRSC from 1-5% in the initial cHL tissue to 40-80% in sorted populations, which enables robust genetic analysis of cHL by targeted high throughput sequencing and array-CGH.

  • Darius Juskevicius
  • David Jucker
  • Alexandar Tzankov

  Technical Report7 Aug 2018 Laboratory Investigation
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  Enzyme-free digital counting of endogenous circular RNA molecules in B-cell malignancies

  Endogenous circular RNA (circRNA) expression has not been characterized in B-cell malignancies, and current methods for circRNA quantification have several limitations. Here, the authors provide a map of circRNA expression in B-cell malignancies based on high-throughput RNA sequencing and present an enzyme-free digital counting methodology, which has the potential to become the gold standard for circRNA quantification.

  • Mette Dahl
  • Iben Daugaard
  • Lasse Sommer Kristensen

  Technical ReportOpen Access7 Aug 2018 Laboratory Investigation
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  Immune checkpoint inhibitors in sarcomas: in quest of predictive biomarkers

  • Robin Veenstra
  • Marie Kostine
  • Judith VMG Bovée

  Mini Review20 Nov 2017 Laboratory Investigation
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  Identification of inhibitors regulating cell proliferation and FUS-DDIT3 expression in myxoid liposarcoma using combined DNA, mRNA, and protein analyses

  • David Svec
  • Soheila Dolatabadi
  • Anders Ståhlberg

  Technical ReportOpen Access27 Mar 2018 Laboratory Investigation
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  Development of a simple osteoarthritis model useful to predict in vitro the anti-hypertrophic action of drugs

  In chondrocytes, TGF-β1 increases the expression of hypertrophic genes, suggesting that this treatment could mimic osteoarthritis in vitro. EZH2 and Sik3 inhibition, as well as HIF induction repress the expression of hypertrophy markers in TGF-β stimulated chondrocytes, validating the reliability of this model to predict anti-hypertrophic effects of drugs.

  • Lyess Allas
  • Quitterie Rochoux
  • Catherine Baugé

  Technical Report13 Aug 2019 Laboratory Investigation
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  Molecular similarities and differences from human pulmonary fibrosis and corresponding mouse model: MALDI imaging mass spectrometry in comparative medicine

  • Michaela Aichler
  • Thomas Kunzke
  • Axel Walch

  Research Article16 Oct 2017 Laboratory Investigation
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  Ligation-dependent RT-PCR: a new specific and low-cost technique to detect ALK, ROS, and RET rearrangements in lung adenocarcinoma

  • Nicolas Piton
  • Philippe Ruminy
  • Jean-Christophe Sabourin

  Technical Report18 Dec 2017 Laboratory Investigation
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  Mouse pancreatic ductal organoid culture as a relevant model to study exocrine pancreatic ion secretion

  The authors provide a side-by-side comparison of morphology and function of primary ductal fragments isolated from mouse pancreas and pancreatic organoid cultures. Using state-of-the-art techniques, they demonstrate that pancreatic organoids can be a suitable and robust model to study pancreatic ductal epithelial ion transport in health and disease.

  • Réka Molnár
  • Tamara Madácsy
  • József Maléth

  Technical Report13 Aug 2019 Laboratory Investigation
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  Optimized cultivation of porcine choroid plexus epithelial cells, a blood–cerebrospinal fluid barrier model, for studying granulocyte transmigration

  • Alexa N. Lauer
  • Martin März
  • Christian Schwerk

  Technical Report17 Apr 2019 Laboratory Investigation