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In recent years we have seen continued growth in the field of precision medicine. Developments in technology have yielded the ability to detect many tumour-relevant entities in blood samples, including circulating tumour DNA, RNA, extracellular vesicles, tumour cells and tumour-educated immune cells. This Collection on Liquid biopsy features Special Issue content guest edited by Professor Angela Cox (University of Sheffield, UK,) and Professor Klaus Pantel (University Medical Center Hamburg-Eppendorf, Germany) and includes a curated set of research articles and commissioned content from leading researchers.