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npj Biofilms and Microbiomes is celebrating five years of publishing the most important scientific advances on microbial biofilms and microbiomes, from cell-to-cell communication and single-cell interaction with the environment, all the way to the human, animal and plant microbiomes, as well as the microbiomes of the natural and built environments. In honor of the occasion, we have selected a collection of top articles that span the spectrum of published work over the past five years. We hope you enjoy reading them.