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Generation of an adenoviral vector containing an addition of a heterologous ligand to the serotype 3 fiber knob

Cancer Gene Therapy volume 10, pages 121–124 (2003)Cite this article

Abstract

As an initial assessment of the feasibility of employing the adenovirus serotype 3 (Ad3) fiber knob as a locale for introducing a tropism-modifying motif, we generated an adenoviral vector containing a six-histidine tag genetically fused to the carboxy-terminus of the Ad3 fiber knob. The heterologous tag proved to be accessible for binding in the context of the virion and, moreover, had rendered the modified vector capable of mediating gene transfer through an artificial, non-Ad3 receptor.

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Acknowledgements

We thank the CFAR DNA Sequencing Core at the University of Alabama at Birmingham. V N Krasnykh is thanked for providing the plasmid pNEB.PK.F5/3. This work was supported by the Dutch Cancer Society, Bekker-la-Bastide fonds, Schuurman-Shimmel van Outeren Stichting, Dittmerfonds, Stichting Korinthiers, the Juvenile Diabetes Foundation (JDF-I-2000-23), and the National Institutes of Health (N01 CO-97110, P50 CA89019, R01 HL67962, R01 CA68245, R01 CA74242, R01 CA86881, R03 CA9054 7, and U19 DK57858).

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Authors and Affiliations

  1. Departments of Medicine, Division of Human Gene Therapy, Pathology and Surgery, and the Gene Therapy Center, University of Alabama at Birmingham, Birmingham, 35294, Alabama, USA

    Taco G Uil, Toshiro Seki, Igor Dmitriev, Elena Kashentseva, Joanne T Douglas & David T Curiel

  2. Department of Therapeutic Gene Modulation, University Centre for Pharmacy, University of Groningen, Groningen, The Netherlands

    Marianne G Rots

  3. Department of Pathology, University Hospital Vrije Universiteit, Amsterdam, The Netherlands

    Jaap M Middeldorp

Authors
  1. Taco G Uil
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  2. Toshiro Seki
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  3. Igor Dmitriev
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  4. Elena Kashentseva
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  5. Joanne T Douglas
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  7. Jaap M Middeldorp
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  8. David T Curiel
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Correspondence to David T Curiel.

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Uil, T., Seki, T., Dmitriev, I. et al. Generation of an adenoviral vector containing an addition of a heterologous ligand to the serotype 3 fiber knob. Cancer Gene Ther 10, 121–124 (2003). https://doi.org/10.1038/sj.cgt.7700543

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