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Graft-versus-Host Disease

Treatment of refractory acute GVHD with third-party MSC expanded in platelet lysate-containing medium

Bone Marrow Transplantation volume 43, pages 245–251 (2009)Cite this article

Abstract

Mesenchymal stem cells have been shown to mediate immunomodulatory effects. They have been used in patients with steroid-refractory acute GVHD (aGVHD), but their relevance as a therapeutic agent targeting aGVHD has still to be defined. In this case series, we report 13 patients with steroid-refractory aGVHD who received BM-derived MSC expanded in platelet lysate-containing medium from unrelated HLA disparate donors. MSC were characterized by their morphological, phenotypical and functional properties. All tested preparations suppressed the proliferation of in vitro activated CD4+ T cells. MSC were transfused at a median dosage of 0.9 × 106/kg (range 0.6–1.1). The median number of MSC applications was 2 (range 1–5). Only two patients (15%) responded and did not require any further escalation of immunosuppressive therapy. Eleven patients received additional salvage immunosuppressive therapy concomitant to further MSC transfusions, and after 28 days, five of them (45%) showed a response. Four patients (31%) are alive after a median follow-up of 257 days, including one patient who initially responded to MSC treatment. In our patient cohort, response to MSC transfusion was lower than in the series reported earlier. However, our experience supports the potential efficacy of MSC in the treatment of steroid-refractory aGVHD.

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Acknowledgements

This work was supported by the Centre for Regenerative Therapy Dresden (CRTD). MB and GE received additional support from the Deutsche Forschungsgemeinschaft (SFB 655; from cells to tissues).

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Authors and Affiliations

  1. Medizinische Klinik und Poliklinik I, University Hospital Carl-Gustav-Carus, Dresden, Germany

    M von Bonin, F Stölzel, A Goedecke, K Richter, N Wuschek, K Hölig, U Platzbecker, T Illmer, M Schaich, J Schetelig, A Kiani, R Ordemann, G Ehninger & M Bornhäuser

  2. Institute for Immunology, Technical University of Dresden, Dresden, Germany

    M Schmitz

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  1. M von Bonin
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  2. F Stölzel
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Correspondence to M Bornhäuser.

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Supplementary Information accompanies the paper on Bone Marrow Transplantation website (http://www.nature.com/bmt)

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von Bonin, M., Stölzel, F., Goedecke, A. et al. Treatment of refractory acute GVHD with third-party MSC expanded in platelet lysate-containing medium. Bone Marrow Transplant 43, 245–251 (2009). https://doi.org/10.1038/bmt.2008.316

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  • DOI: https://doi.org/10.1038/bmt.2008.316

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