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Expression and polymorphism (rs4880) of mitochondrial superoxide dismutase (SOD2) and asparaginase induced hepatotoxicity in adult patients with acute lymphoblastic leukemia

The Pharmacogenomics Journal volume 17, pages 274–279 (2017)Cite this article

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Abstract

Asparaginase, which depletes asparagine and glutamine, activates amino-acid stress response. Oxidative stress mediated by excessive reactive oxygen species (ROS) causes enhanced mitochondrial permeabilization and subsequent cell apoptosis and is considered as a plausible mechanism for drug-induced hepatotoxicity, a common toxicity of asparaginase in adults with acute lymphoblastic leukemia (ALL). Studies investigating the pharmacogenetics of asparaginase in ALL are limited and focused on asparaginase-induced allergic reaction common in pediatric patients. Here, we sought to determine a potential association between the variant rs4880 in SOD2 gene, a key mitochondrial enzyme that protects cells against ROS, and hepatotoxicity during asparaginase-based therapy in 224 patients enrolled on CALGB-10102, a treatment trial for adults with ALL. We report that the CC genotype of rs4880 is associated with increased hepatotoxicity following asparaginase-based treatment. Thus, rs4880 likely contributes to asparaginase-induced hepatotoxicity, and functional studies investigating this single-nucleotide polymorphism (SNP) are needed to develop therapeutic approaches that mitigate this toxicity.

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Acknowledgements

We would like to thank the University of Chicago Cancer Research Foundation Women’s Board and Division of Biological Sciences. This work was also supported by the following grants: NIH grants: T32GM007019, UM1 CA186705 and P30 CA14599-36.

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Authors and Affiliations

  1. Department of Pharmacy, USC School of Pharmacy, University of Southern California, Los Angeles,, CA, USA

    H Alachkar

  2. Department of Medicine, Section of Hematology/Oncology, University of Chicago, Chicago,, IL, USA

    N Fulton, G Malnassy, M Mutonga, R A Larson, Y Nakamura & W Stock

  3. Alliance/CALGB Statistical Center, Duke Cancer Institute, Biostatistics,, Durham,, NC, USA

    B Sanford

  4. Division of Hematology, Department of Medicine, The Ohio State University, Columbus,, OH, USA

    C D Bloomfield

  5. Department of Hematology and Hematopoietic Cell Transplantation, Gehr Family Center for Leukemia, City of Hope Comprehensive Cancer Center, Duarte,, CA, USA

    G Marcucci

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  1. H Alachkar
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Correspondence to W Stock.

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Alachkar, H., Fulton, N., Sanford, B. et al. Expression and polymorphism (rs4880) of mitochondrial superoxide dismutase (SOD2) and asparaginase induced hepatotoxicity in adult patients with acute lymphoblastic leukemia. Pharmacogenomics J 17, 274–279 (2017). https://doi.org/10.1038/tpj.2016.7

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