Figure 2

From: Hippocampal encoding of interoceptive context during fear conditioning

Figure 2

EtOH enhances phosphorylation of GluR1 at serine 845 in hippocampus. Representative immunoblots of GluR1, GluR1S845, GluR1S831 and quantitative analysis of band density from HIP and LA following the indicated treatment conditions. (a, d) Twenty-four hours after FC (lanes indicated+are fear conditioned, and lanes indicated− are sham control). (b,e) Twenty-four hours after FC including 2 h of exposure to binge EtOH intake (ETOH) or water. (c,f) Twenty-four hours after FC including 2 h of exposure to binge EtOH intake or water, and following the retrieval cue (Ret; tone). (g,i) Immediately following extinction for the indicated treatment groups. (h, j) Twenty-four hours following extinction and immediately following the fear relapse test. Representative traces show relative changes in protein expression of GluR1, GluR1S831 and GluR1S845 for the indicated treatment conditions in HIP (k) and LA (l). Data are mean±s.d. *P<0.05, **P<0.01 compared with the indicated treatment condition. EtOH, ethanol; FC, fear conditioning; HIP, hippocampus; LA, lateral amygdala.