Abnormal seizure response to PTZ in Df(h15q13)−/− mice. Mice received PTZ 40 mg kg−1 subcutaneously and were observed for 30 min and scored for seizures as described in Materials and Methods. n=5–7 for each genotype. (a) Time in early-stage seizure. Kruskal–Wallis test, P<0.05. Dunn’s multiple comparisons induicated. (b) Number of myoclonic jerks during a 30-min period after PTZ administration. Kruskal–Wallis test, P<0.01. Dunn’s multiple comparisons indicated. (c) Incidence of clonic seizures. Logrank test, P<0.01. WT vs Df(h15q13)+/− NS, WT vs Df(h15q13)−/− P<0.01, Df(h15q13)+/− vs Df(h15q13)−/− NS. Data (a, b) presented as mean±s.e.m. **P<0.01. NS, not significant; PTZ, pentylene tetrazole; WT, wildtype.