This Article contains typographical errors in the Methods section under subheading ‘WNT10B/WNT10BIVS1 Gene expression analysis’.
“The WNT10B (P4-P2 primers) amplification was performed with following thermal conditions: 94 °C for 1 min, 33 cycles at 94° for 30 s, 58 °C for 30 s, 72 °C for 30 s and 72 °C for 5 min. The amplification of WNT10BIVS1 (P3-P2 primers) was performed as follows: 94 °C for 1 min, 33 cycles at 94° for 30 s, 61 °C for 30 s, 72 °C for 30 s and 72 °C for 5 min”.
should read:
“The WNT10B (P4-P1 primers) amplification was performed with following thermal conditions: 94 °C for 1 min, 33 cycles at 94° for 30 s, 58 °C for 30 s, 72 °C for 30 s and 72 °C for 5 min. The amplification of WNT10BIVS1 (P3-P1 primers) was performed as follows: 94 °C for 1 min, 33 cycles at 94° for 30 s, 61 °C for 30 s, 72 °C for 30 s and 72 °C for 5 min”.
In the same section, under subheading ‘WNT10B/WNT10BIVS1 Absolute quantification’,
“We performed the experiment on Bio-Rad’s QX100 ddPCR system and the reaction mixtures in a final 20 μl volume consisted of 10 μl of 2 × One-Step RT-ddPCR Supermix (Bio-Rad, CA, USA), 1 mM Manganese Acetate solution (Bio-Rad, CA, USA), 0.5 μM of primers (WNT10B: P4-P2, WNT10BIVS1 P3-P2), 0.25 μM WNT10B_dd1 and WNT10BIVS1_dd2 probes”.
should read:
“We performed the experiment on Bio-Rad’s QX100 ddPCR system and the reaction mixtures in a final 20 μl volume consisted of 10 μl of 2 × One-Step RT-ddPCR Supermix (Bio-Rad, CA, USA), 1 mM Manganese Acetate solution (Bio-Rad, CA, USA), 0.5 μM of primers (WNT10B: P4-P1, WNT10BIVS1 P3-P1), 0.25 μM WNT10B_dd1 and WNT10BIVS1_dd2 probes”.
Additional information
The online version of the original article can be found at 10.1038/srep37201
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Lazzaroni, F., Giacco, L., Biasci, D. et al. Correction: Corrigendum: Intronless WNT10B-short variant underlies new recurrent allele-specific rearrangement in acute myeloid leukaemia. Sci Rep 7, 46788 (2017). https://doi.org/10.1038/srep46788
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DOI: https://doi.org/10.1038/srep46788
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