Figure 2 : The survival rates of each strain following acid challenge analyzed by Flow cytometry.

From: Role of the virulence plasmid in acid resistance of Shigella flexneri

Figure 2

(A) Flow cytometry was used to provide counts of living cells before and after acid treatment, calculate the viability of bacterial cells, and then infer the strength of the acid tolerance. R1 represented the whole cells; R2–R4 were set around the dead, injured, and live bacterial populations, respectively. (B) Survival rates of bacterial cells were calculated following culture in acid medium. Injured cells also counted as surviving cells.