Figure 1: B cells induced IL-10-producing T cells without additional IL-10. | Scientific Reports

Figure 1: B cells induced IL-10-producing T cells without additional IL-10.

From: Characterization of c-Maf+Foxp3 Regulatory T Cells Induced by Repeated Stimulation of Antigen-Presenting B Cells

Figure 1

(A) CD4+CD25 T cells cultured with OVA323–339 peptide-pulsed B cells (Day 0, 7, 14) for 3 days and then cells rested in medium supplement with recombinant IL-2 (Day 3 and 10) for 4 days. Treg-of-B (ToB)-1, -2, and -3 cells were collected by depleting B cells. (B) The suppressive assay. Splenic CD4+CD25 (T) cells seeded as responder cells with suppressor cells, including Treg-of-B cells and splenic CD4+CD25+ (Treg) cells, at a ratio of 1:1. Data shown were the representative of 3 individual experiments. (C) Representative intracellular cytokine staining in splenic naïve CD4+CD25 (T) and Treg-of-B cells. (D) The cumulative data of frequency of IL-10-, IFN-γ- and IL-4-producing populations in reactivated Treg-of-B cells from 4–6 individual experiments.

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