(a) A sagittal view of the hemisphere. (b–d) Magnified images of the boxed areas in (a). (b) Hippocampus (HIP); (c) Corpus callosum (CC) and caudoputamen (CP); (d) Midbrain (MB). (e) A three-dimensional rendering of the CAST volumetric image stack. (See Supplementary Movie 1 for a movie.) (f) The corresponding sagittal image from a light-sheet microscope on the same hemisphere stained by DiD. (b–d) share the same scale bar in (c).