Cells were incubated with different concentrations of corylin for 1 h, and then were treated with LPS (1 μg/mL) for 30 min. (A) The expression levels of phosphor-JNK 1/2, JNK 1/2, phosphor-p38 MAPK, p38 MAPK, phosphor-ERK 1/2 and ERK 1/2 were determined by western blot. (B) Band intensities were quantified from three independent experiments. The relative fold of phosphorylation activity was normalized to that of the un-phosphorylated form and compared to untreated samples. (C) J-Blue cells were incubated with different concentrations of corylin for 1 h, and then were treated with LPS (1 μg/mL) for 24 h. The activity of NF-κB was measured by NF-κB promoter reporter assay. The data are presented as the means ± SD of three independent experiments. Statistical significance was assessed by one-way ANOVA followed by Tukey post-hoc test and represented as follows: *p < 0.05 and **p < 0.01 vs. LPS alone.