Figure 4: Effects of corylin on the expression and phosphorylation of JNK 1/2, p38 MAPK and ERK 1/2 by LPS-activated RAW 264.7 cells and the activation of NF-κB by LPS-activated J-Blue cells. | Scientific Reports

Figure 4: Effects of corylin on the expression and phosphorylation of JNK 1/2, p38 MAPK and ERK 1/2 by LPS-activated RAW 264.7 cells and the activation of NF-κB by LPS-activated J-Blue cells.

From: Corylin protects LPS-induced sepsis and attenuates LPS-induced inflammatory response

Figure 4

Cells were incubated with different concentrations of corylin for 1 h, and then were treated with LPS (1 μg/mL) for 30 min. (A) The expression levels of phosphor-JNK 1/2, JNK 1/2, phosphor-p38 MAPK, p38 MAPK, phosphor-ERK 1/2 and ERK 1/2 were determined by western blot. (B) Band intensities were quantified from three independent experiments. The relative fold of phosphorylation activity was normalized to that of the un-phosphorylated form and compared to untreated samples. (C) J-Blue cells were incubated with different concentrations of corylin for 1 h, and then were treated with LPS (1 μg/mL) for 24 h. The activity of NF-κB was measured by NF-κB promoter reporter assay. The data are presented as the means ± SD of three independent experiments. Statistical significance was assessed by one-way ANOVA followed by Tukey post-hoc test and represented as follows: *p < 0.05 and **p < 0.01 vs. LPS alone.

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