Figure 2: Effects of corylin on the production of TNF-α, IL-6 and HMGB1 by LPS-activated RAW 264.7 cells. | Scientific Reports

Figure 2: Effects of corylin on the production of TNF-α, IL-6 and HMGB1 by LPS-activated RAW 264.7 cells.

From: Corylin protects LPS-induced sepsis and attenuates LPS-induced inflammatory response

Figure 2

Cells were incubated with different concentrations of corylin for 1 h, and then were treated with LPS (1 μg/mL) for 24 h. The levels of (A) TNF-α and (B) IL-6 (C) HMGB1 in the cell culture supernatant were measured by ELISA. The data are presented as the means ± SD of three independent experiments. Statistical significance was assessed by one-way ANOVA followed by Tukey post-hoc test and represented as follows: *p < 0.05 and **p < 0.01 vs. LPS alone. (D) Cells were incubated with different concentrations of corylin for 1 h, and then were treated with LPS (1 μg/mL) for 6 h. The expression of HMGB1 in both cytosol and nucleus was determined by western blot. The Western blotting results are representative of results obtained in three separate experiments.

Back to article page