Figure 6 : Klotho is essential for the bone protection by HDAC inhibition.

From: Klotho preservation via histone deacetylase inhibition attenuates chronic kidney disease-associated bone injury in mice

Figure 6

(A) Representative H&E staining of mouse distal femur sections from siRNA-control or siRNA-Klotho-injected control, TSA, adenine and TSA-treated adenine mice (6 weeks, n = 6 in each group). (B) Average bone mRNA levels of Runx2 and Spp1 examined by qRT-PCR (n = 6). (C) Representative micro-CT 3D images of trabecular architectures of the distal femurs from mice as in Fig. 6A. (D) Quantitative analyses of the ratio of bone volume to tissue volume (BV/TV), trabecular number (Tb.N), trabecular thickness (Tb.Th) and trabecular separation (Tb.Sp) from micro-CT examinations (n = 6). Data are presented as the mean ± SD. *P < 0.05, **P < 0.01 versus control, #P < 0.05, ##P < 0.01 versus adenine treatment in siR-control mice; ΔP < 0.05, ΔΔP < 0.01 versus control in siR-Klotho mice.