Figure 4 : Engineering C2E for elevated lactulose production.

From: Design and application of a lactulose biosensor

Figure 4

(a) The BLGR strains co-expressing LacI-L5 and the C2E random mutagenesis library grown on LB agar supplemented with 10 mM lactose under UV 302 nm. (b) Lactulose productions of the selected variants expressing the C2E mutants at 37 °C, compared with that of strain expressing the wild-type C2E (C2E-WT). (c) Western-blot analysis of mutant C2 expression as compared with the wild-type enzyme. (d) Specific activities of wild-type C2E (C2E-WT) and mutant C2 at 37 °C and 80 °C.