Figure 2 : Engineering LacI for altered effector specificity.

From: Design and application of a lactulose biosensor

Figure 2

(a) GFP fluorescence from strain BLGR harboring plasmid pLac7-L5 as a function of lactulose. The corresponding dose-response curve of wild-type LacI (LacI-WT) is included for reference. (b) GFP fluorescence from the strain BLGR, which harbors plasmid pLac7-L5, in response to 1 mM of the indicated disaccharides, and the structures of the disaccharides. (c) The binding affinity of LacI-L5 for lactulose was measured by SPR. The corresponding binding affinity of wild-type LacI (LacI-WT) is included for reference.