Table 1: Sequences of the oligo used for the generation and confirmation of 293T-SNCA-3′NL cells.

From: A novel tool for monitoring endogenous alpha-synuclein transcription by NanoLuciferase tag insertion at the 3′end using CRISPR-Cas9 genome editing technique

Name of the oligosSequence (5′ to 3′)
SNCA sgRNA Top StrandCACCGTGGGAGCAAAGATATTTCTT
SNCA sgRNA Bottom StrandAAACAAGAAATATCTTTGCTCCCAC
Upstream Homology Forward PrimerTATGGCGGCCGCTTAGGAACAAGGAAAAT
Upstream Homology Reverse PrimerAGTGAGCTCGGCTTCAGGTTCGTAGTC
NanoLuc Forward PrimerAAAGAGCTCATGGTCTTCACACTCGAA
NanoLuc Reverse PrimerCATGACGTCAAGCTTTTACGCCAGAATGCGTG
Downstream Homology Forward PrimerAAGCTTAAATATCTTTGCTCCCAGTTTCTTGA
Downstream Homology Reverse PrimerGTTGACGTCGCGGCCGCATACCAAAACA
Insertion Confirmation Forward PrimerCTGCAGAATATTTGCAAAAACATTGATTG
Insertion Confirmation Reverse PrimerTAAAAACTTTGAGAAATGTCATGACTGGG
cDNA sequencing Forward PrimerGGAGTGGCCATTCGACGACAGTG
cDNA sequencing Reverse PrimerTAAAAACTTTGAGAAATGTCATGACTGGG
SNCA Exon 4 Forward PrimerCAAATGTTGGAGGAGCAGTGGTGA
NanoLuc Internal Forward PrimerAAGGTGATCCTGCACTATGGCA