A–D) RLI images through the cranial window and pseudocolored difference maps showing the peak responses evoked by 3 concentrations of BA from a Sham-male (A, GNX01), a Sham-female ( B), GNX13), a Gnx-male ( C), GNX28), and a Gnx-female ( D), GNX04). (E– H) Fluorescent records from individual trials that correspond to the glomerular callouts that are noted by white arrows on the 30 au BA-evoked maps in ( A– D). The boxed portion of the fluorescent records indicates the frames that were used to generate response maps in ( A– D) and analyses summarized in ( I, J, L and M). The example piezosensor recordings that are shown below each response amplitude are from a single trial of 30 au BA. Positive and negative portions of each respiration trace respectively correspond to inhalation (in) and exhalation (ex) phases of the respiratory cycle. ( I) Odorant-evoked glomerular responses during the peak response phase are pooled across all odorants and concentrations and plotted separately for each group. a indicates p = 0.008 when compared with Sham-male and Gnx-female groups; b indicates p = 0.001 when compared with Sham-male and Gnx-female groups. ( J) Odorant-evoked glomerular responses are pooled across odorants and plotted as a function of concentration for each group. ( K) Odorant-evoked glomerular responses are plotted as a function of time relative to stimulus presentation (yellow stimulus bar). ( L) The number of odorants that evoked a measurable response in each glomerulus are plotted for sex × surgical treatment glomerular populations. Ns indicate the number of glomeruli per group. Note that each individual glomerulus was categorized as responding to 1, 2, 3, or 4 odorants in the panel, but the y-axis is truncated at 2.5 to display an appropriate range for the group means. ( M) Peak odorant-evoked change in fluorescence (ΔF) pooled across all odorants and concentrations for each group. ( N) Inhalation frequency during 6 sec odorant presentations for each group. Data are pooled across respiration measurements that were recorded during trials from each concentration of each odorant. The data shown in ( I– N) are plotted as the mean ± SEM.