Figure 4 : Sexually dimorphic activation of olfactory bulb glomeruli is dependent upon circulating gonadal hormones.

From: Differences in peripheral sensory input to the olfactory bulb between male and female mice

Figure 4

(A–D) RLI images through the cranial window and pseudocolored difference maps showing the peak responses evoked by 3 concentrations of BA from a Sham-male (A, GNX01), a Sham-female (B), GNX13), a Gnx-male (C), GNX28), and a Gnx-female (D), GNX04). (EH) Fluorescent records from individual trials that correspond to the glomerular callouts that are noted by white arrows on the 30 au BA-evoked maps in (AD). The boxed portion of the fluorescent records indicates the frames that were used to generate response maps in (AD) and analyses summarized in (I,J,L and M). The example piezosensor recordings that are shown below each response amplitude are from a single trial of 30 au BA. Positive and negative portions of each respiration trace respectively correspond to inhalation (in) and exhalation (ex) phases of the respiratory cycle. (I) Odorant-evoked glomerular responses during the peak response phase are pooled across all odorants and concentrations and plotted separately for each group. a indicates p = 0.008 when compared with Sham-male and Gnx-female groups; b indicates p = 0.001 when compared with Sham-male and Gnx-female groups. (J) Odorant-evoked glomerular responses are pooled across odorants and plotted as a function of concentration for each group. (K) Odorant-evoked glomerular responses are plotted as a function of time relative to stimulus presentation (yellow stimulus bar). (L) The number of odorants that evoked a measurable response in each glomerulus are plotted for sex × surgical treatment glomerular populations. Ns indicate the number of glomeruli per group. Note that each individual glomerulus was categorized as responding to 1, 2, 3, or 4 odorants in the panel, but the y-axis is truncated at 2.5 to display an appropriate range for the group means. (M) Peak odorant-evoked change in fluorescence (ΔF) pooled across all odorants and concentrations for each group. (N) Inhalation frequency during 6 sec odorant presentations for each group. Data are pooled across respiration measurements that were recorded during trials from each concentration of each odorant. The data shown in (IN) are plotted as the mean ± SEM.